Circulating tumor cell isolation for cancer diagnosis and prognosis

Deng, Zicheng; Wu, Sheng‐Ming; Wang, Yilong; Shi, Donglu

doi:10.1016/j.ebiom.2022.104237

Cited by 121 publications

(99 citation statements)

References 130 publications

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“…EpCAM-based methods have been previously considered the gold standard for CTCs detection [14][15][16]. Nevertheless, tumors could be characterized by low EpCAM expression or be EpCAM-negative, as in the case of malignant melanoma and medulloblastoma [17][18][19][20][21][22][23][24].Furthermore, due to the lost expression of EpCAM during EMT, conventional EpCAM-based methods could be inefficient in capturing CTCs [25][26][27].More sensitive biomarker-independent CTC isolation techniques have been recently developed with surface-charged superparamagnetic nanoprobes capable of different EMT subpopulation CTC capture from a tiny volume of blood [28,29]. Moreover, the development of sophisticated single-cell analysis technologies has allowed the dissection of heterogeneity within CTCs [29].…”

Section: Liquid Biopsymentioning

confidence: 99%

“…Furthermore, due to the lost expression of EpCAM during EMT, conventional EpCAM-based methods could be inefficient in capturing CTCs [ 25 , 26 , 27 ]. More sensitive biomarker-independent CTC isolation techniques have been recently developed with surface-charged superparamagnetic nanoprobes capable of different EMT subpopulation CTC capture from a tiny volume of blood [ 28 , 29 ]. Moreover, the development of sophisticated single-cell analysis technologies has allowed the dissection of heterogeneity within CTCs [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

“…More sensitive biomarker-independent CTC isolation techniques have been recently developed with surface-charged superparamagnetic nanoprobes capable of different EMT subpopulation CTC capture from a tiny volume of blood [ 28 , 29 ]. Moreover, the development of sophisticated single-cell analysis technologies has allowed the dissection of heterogeneity within CTCs [ 29 ]. CTC from liquid biopsies can provide non-invasive diagnostic and therapeutic information for several cancer types.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, CTCs isolated from CRC patients harbored KRAS mutations, which are a strong predictor ofresistance to EGFR inhibitors [ 30 ]. Nevertheless, critical issues remain in CTC detection sensitivity, technical reproducibility, and identification accuracy [ 29 ] ( Table 1 ).…”

Section: Introductionmentioning

confidence: 99%

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Circulating Histones to Detect and Monitor the Progression of Cancer

Tsoneva

Ivanov

Conev

et al. 2023

IJMS

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Liquid biopsies have emerged as a minimally invasive cancer detection and monitoring method, which could identify cancer-related alterations in nucleosome or histone levels and modifications in blood, saliva, and urine. Histones, the core component of the nucleosome, are essential for chromatin compaction and gene expression modulation. Increasing evidence suggests that circulating histones and histone complexes, originating from cell death or immune cell activation, could act as promising biomarkers for cancer detection and management. In this review, we provide an overview of circulating histones as a powerful liquid biopsy approach and methods for their detection. We highlight current knowledge on circulating histones in hematologic malignancies and solid cancer, with a focus on their role in cancer dissemination, monitoring, and tumorigenesis. Last, we describe recently developed strategies to identify cancer tissue-of-origin in blood plasma based on nucleosome positioning, inferred from nucleosomal DNA fragmentation footprint, which is independent of the genetic landscape.

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Section: Liquid Biopsymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Circulating Histones to Detect and Monitor the Progression of Cancer

Tsoneva

Ivanov

Conev

et al. 2023

IJMS

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“…In conclusion, we have investigated the effects of magnetic dipole interactions on the dynamic loss mechanisms for medical therapeutics [ 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 36 , 37 , 38 , 39 , 40 , 41 ]. It should be noted that iron oxide nanoparticles have also been applied recently for solar harvesting and energy generation in multifunctional building skins [ 1 , 42 , 43 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of Dipole Interactions on Blocking Temperature and Relaxation Dynamics of Superparamagnetic Iron-Oxide (Fe3O4) Nanoparticle Systems

et al. 2023

Self Cite

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The effects of dipole interactions on magnetic nanoparticle magnetization and relaxation dynamics were investigated using five nanoparticle (NP) systems with different surfactants, carrier liquids, size distributions, inter-particle spacing, and NP confinement. Dipole interactions were found to play a crucial role in modifying the blocking temperature behavior of the superparamagnetic nanoparticles, where stronger interactions were found to increase the blocking temperatures. Consequently, the blocking temperature of a densely packed nanoparticle system with stronger dipolar interactions was found to be substantially higher than those of the discrete nanoparticle systems. The frequencies of the dominant relaxation mechanisms were determined by magnetic susceptibility measurements in the frequency range of 100 Hz–7 GHz. The loss mechanisms were identified in terms of Brownian relaxation (1 kHz–10 kHz) and gyromagnetic resonance of Fe3O4 (~1.12 GHz). It was observed that the microwave absorption of the Fe3O4 nanoparticles depend on the local environment surrounding the NPs, as well as the long-range dipole–dipole interactions. These significant findings will be profoundly important in magnetic hyperthermia medical therapeutics and energy applications.

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Long non‐coding RNAFOXD1‐AS1 modulated CTCs epithelial‐mesenchymal transition and immune escape in hepatocellular carcinoma in vitro by sponging miR‐615‐3p

Guo,

Zheng,

Jiang

et al. 2024

Cancer Reports

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BackgroundHepatocellular carcinoma (HCC) is widely recognized as a globally prevalent malignancy. Immunotherapy is a promising therapy for HCC patients. Increasing evidence suggests that lncRNAs are involved in HCC progression and immunotherapy.AimThe study reveals the mechanistic role of long non‐coding RNA (lncRNA) FOXD1‐AS1 in regulating migration, invasion, circulating tumor cells (CTCs), epithelial‐mesenchymal transition (EMT), and immune escape in HCC in vitro.MethodsThis study employed real‐time PCR (RT‐qPCR) to measure FOXD1‐AS1, miR‐615‐3p, and programmed death‐ligand 1 (PD‐L1). The interactions of FOXD1‐AS1, miR‐615‐3p, and PD‐L1 were validated via dual‐luciferase reporter gene and ribonucleoprotein immunoprecipitation (RIP) assay. In vivo experimentation involves BALB/c mice and BALB/c nude mice to investigate the impact of HCC metastasis.ResultsThe upregulation of lncRNA FOXD1‐AS1 in malignant tissues significantly correlates with poor prognosis. The investigation was implemented on the impact of lncRNA FOXD1‐AS1 on the migratory, invasive, and EMT of HCC cells. It has been observed that the lncRNA FOXD1‐AS1 significantly influences the generation and metastasis of MCTC in vivo analysis. In mechanistic analysis, lncRNA FOXD1‐AS1 enhanced immune escape in HCC via upregulation of PD‐L1, which acted as a ceRNA by sequestering miR‐615‐3p. Additionally, lncRNA FOXD1‐AS1 was found to modulate the EMT of CTCs through the activation of the PI3K/AKT pathway.ConclusionThis study presents compelling evidence supporting the role of lncRNA FOXD1‐AS1 as a miRNA sponge that sequesters miR‐655‐3p and protects PD‐L1 from suppression.

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Circulating tumor cell isolation for cancer diagnosis and prognosis

Cited by 121 publications

References 130 publications

Circulating Histones to Detect and Monitor the Progression of Cancer

Circulating Histones to Detect and Monitor the Progression of Cancer

Effect of Dipole Interactions on Blocking Temperature and Relaxation Dynamics of Superparamagnetic Iron-Oxide (Fe3O4) Nanoparticle Systems

Long non‐coding RNAFOXD1‐AS1 modulated CTCs epithelial‐mesenchymal transition and immune escape in hepatocellular carcinoma in vitro by sponging miR‐615‐3p

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