Circulating tumor cell enumeration for improved screening and disease detection of patients with colorectal cancer

Tsai, Wen‐Sy; Hung, Wei-Shan; Wang, Tzu-Min; Liu, Hsuan; Yang, Chao-Ping; Wu, Shao‐Min; Hsu, Hsueh-Ling; Hsiao, Yu-Chiao; Tsai, Hui‐Ju; Tseng, Ching Ping

doi:10.1016/j.bj.2020.09.006

Cited by 12 publications

(8 citation statements)

References 69 publications

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“…∼1 cell per mL). While the positive detection threshold for cancer is commonly set at ≥ 2 CK + /CD45 - scCTCs per 7.5 mL of blood 60 , epithelial non-CTCs are commonly detected from the blood of healthy individuals at cell counts from 1.5 to 2 cells per mL 61,62 , and reaching as high as 21 cells per mL 63 , and has been ascribed to shedding of epithelial cells into the bloodstream and/or blood collection device during venipuncture 64 .…”

Section: Resultsmentioning

confidence: 99%

Gravity-based microfiltration reveals unexpected prevalence of circulating tumor cell clusters in ovarian and colorectal cancer

Meunier

Hernández-Castro

Kheireddine

et al. 2019

Preprint

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Circulating tumor cells (CTCs) are rare (few cells per milliliter of blood) and mostly isolated as single cell CTCs (scCTCs). CTC clusters (cCTCs), even rarer, are of growing interest, notably because of their higher metastatic potential, but very difficult to isolate. Here, we introduce gravity-based microfiltration (GµF) for facile isolation of cCTCs. We identify cluster break-up as a confounding cause, and achieve ~85% capture efficiency. GµF from orthotopic ovarian cancer mouse models and from 10 epithelial ovarian cancer (EOC) patients uncovered cCTCs in every case, with between 2-100+ cells. cCTCs represented between 5-30% of all CTC events, and 10-80% of captured CTCs were clustered; remarkably, in two patients, more CTCs were circulating as cCTCs than scCTCs. GµF uncovered the unexpected prevalence, frequency and sometimes large size of cCTCs in EOC patients with either metastatic and localized disease, and motivates additional studies to uncover their properties and role in disease progression.

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Section: Resultsmentioning

confidence: 99%

Gravity-based microfiltration reveals unexpected prevalence of circulating tumor cell clusters in ovarian and colorectal cancer

Meunier

Hernández-Castro

Kheireddine

et al. 2019

Preprint

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“…In the case of CTCs, variations in detection cut-off values, cancer status, previous therapeutic interventions, and detection methods across studies, along with the nonspecific markers used for CTC detection, such as EpCAM, PDPN, and TSHR, affect the reliability of results (Zhang et al 2009, Williams 2011, Tsai et al 2020. Despite this, CTCs may still prove helpful in monitoring disease status and recurrence in DTC patients, especially after lobectomy, in conjunction with Tg and TgAb.…”

Section: Discussionmentioning

confidence: 99%

Liquid biopsies in thyroid cancers: a systematic review and meta-analysis

Zeyghami,

Hansen,

Jakobsen

et al. 2023

Endocrine-Related Cancer

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Thyroid cancer (TC) represents the most common endocrine malignant tumor. Liquid biopsy has been suggested as a new and accurate biomarker in cancer. This systematic review analyzes the existing literature on circulating tumor cells (CTCs), circulating tumor DNA (ctDNA), cell-free DNA integrity index (cfDI), and their potential as biomarkers for TC, including the subtypes: differentiated (papillary and follicular), medullary and anaplastic. A systematic search was performed in Pubmed, Embase, and Cochrane databases for published articles in English between January 1st 1970 and September 6th 2022 (PROSPERO: CRD42022358592). The literature search generated a total of 635 articles. In total, 36 articles were included (patients = 2566). Four studies reported that higher levels of CTCs were associated with metastases and worse prognosis. Nineteen studies found the presence of mutated ctDNA in TC patients. The diagnostic accuracy in detecting BRAFV600E as ctDNA was determined in eleven studies regarding papillary thyroid cancer. The pooled sensitivity, specificity, and diagnostic odds ratio were estimated at 56% (95 % CI 36-74), 91 % (95 % CI 84-95) and 12 (CI 95 % 4.09-33.11), respectively. Four studies concluded that the cfDI was higher in patients with TC compared to benign thyroid lesions and healthy controls. The detection of CTCs, ctDNA, and cfDI may have a potential prognostic value in TC in relation to diagnosis, disease progression, and treatment efficacy. Despite the promising potential of CTCs, ctDNA, and cfDI in TC management, limitations hinder direct comparison and generalization of findings. Standardized methodologies, larger patient cohorts, and a consensus on relevant markers are needed to validate their clinical applicability and enhance TC manageme

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“…In another article of this issue, Tsai et al. investigated subsets of circulating tumor cells (CTCs) in peripheral blood in order to find additional modalities to complement the above mentioned assays and improve disease detection [ 6 ].…”

Section: Spotlightmentioning

confidence: 99%

“…Tsai et al. conclude that CTC testing delivers distinct results and could thus improve CRC screening by supplementing assays like CEA and iFOBT [ 6 ].…”

Section: Spotlightmentioning

confidence: 99%

The best protection is early detection

Kattner¹

2021

Biomedical Journal

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Circulating tumor cell enumeration for improved screening and disease detection of patients with colorectal cancer

Cited by 12 publications

References 69 publications

Gravity-based microfiltration reveals unexpected prevalence of circulating tumor cell clusters in ovarian and colorectal cancer

Gravity-based microfiltration reveals unexpected prevalence of circulating tumor cell clusters in ovarian and colorectal cancer

Liquid biopsies in thyroid cancers: a systematic review and meta-analysis

The best protection is early detection

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