Circulating polymerase chain reaction chips utilizing multiple-membrane activation

Wang, Chih-Hao; Chen, Yi Yu; Liao, Chia Sheng; Hsieh, Tsung Min; Luo, Ching Hsing; Wu, Jiunn Jong; Lee, Huei Huang; Lee, Gwo‐Bin

doi:10.1088/0960-1317/17/2/024

Cited by 17 publications

(10 citation statements)

References 33 publications

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“…[5] There are two ways of conducting an on-chip PCR. [6][7][8][9] In the time domain, a stationary PCR mixture is thermocycled between three different temperatures. Under time-space conversion, a PCR mixture is driven through a microchannel that is constantly held at three different temperatures.…”

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confidence: 99%

Clockwork PCR Including Sample Preparation

et al. 2008

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For whom the bell tolls: Surface‐functionalized superparamagnetic particles emulsified in mineral oil turn a free‐standing droplet into a flexible virtual laboratory with (sub)microliter volumes. By using magnetic forces, rare acute monocytic leukaemia cells are extracted from blood, preconcentrated, purified, lysed, and subjected to a real‐time PCR in minutes. The PCR works like a clockwork by rotating the droplet over different temperature zones.

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confidence: 99%

Clockwork PCR Including Sample Preparation

et al. 2008

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“…9,11,34,40,47,51,77,90,93 For instance, micromachined flow-through PCR chips consisted of a microfluidic control module and a temperature control module have been demonstrated for fast DNA amplification. The microfluidic control module, which used two different motions of multiple membranes 136 and suction-type membranes 18 for flow transport, as shown in Fig. 3, was used to rapidly transport the DNA samples through the three heating sections.…”

Section: Microcontinuous-flow/flow-through Pcr Chipsmentioning

confidence: 99%

Sample Pretreatment and Nucleic Acid-Based Detection for Fast Diagnosis Utilizing Microfluidic Systems

Wang

Lee

2011

Ann Biomed Eng

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Recently, micro-electro-mechanical-systems (MEMS) technology and micromachining techniques have enabled miniaturization of biomedical devices and systems. Not only do these techniques facilitate the development of miniaturized instrumentation for biomedical analysis, but they also open a new era for integration of microdevices for performing accurate and sensitive diagnostic assays. A so-called "micro-total-analysis-system", which integrates sample pretreatment, transport, reaction, and detection on a small chip in an automatic format, can be realized by combining functional microfluidic components manufactured by specific MEMS technologies. Among the promising applications using microfluidic technologies, nucleic acid-based detection has shown considerable potential recently. For instance, micro-polymerase chain reaction chips for rapid DNA amplification have attracted considerable interest. In addition, microfluidic devices for rapid sample pretreatment prior to nucleic acid-based detection have also achieved significant progress in the recent years. In this review paper, microfluidic systems for sample preparation, nucleic acid amplification and detection for fast diagnosis will be reviewed. These microfluidic devices and systems have several advantages over their large-scale counterparts, including lower sample/reagent consumption, lower power consumption, compact size, faster analysis, and lower per unit cost. The development of these microfluidic devices and systems may provide a revolutionary platform technology for fast sample pretreatment and accurate, sensitive diagnosis.

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“…Detailed information about the microfabrication process can be found in our previous work (Wang et al 2007). Briefly, the circulating PCR chip was made of a soda-lime glass layer (300×400×1.1 mm 3 , G-Tech Optoelectronics Corp., Taiwan) and two PDMS (Sil-More Industrial Ltd., Sylgard 184A and Sylgard 184B) layers.…”

Section: Fabrication Processmentioning

confidence: 99%

“…Compared with other fluid transportation mechanisms such as hydrodynamic-, magnetohydrodynamic-, and electrokinetic-driven microdevices, the pneumatic-driven microdevices are simple to fabricate and control, can be integrated with other functional devices, and good for optical detection . For instance, DNA amplification utilizing a flow-through PCR chip integrated with multiple-membrane activation was reported by the current research group (Wang et al 2007). However, bubble formation is still a serious issue in the enclosed reservoir which adversely affects PCR performance.…”

Section: Introductionmentioning

confidence: 97%

“…In addition, the cycle numbers and the reaction time for the denaturing, annealing, and extension steps can be randomly adjusted by the microfluidic control module. Array-type microheaters with a self-compensation function (Hsieh et al 2007) are adopted to improve the thermal uniformity inside the PCR chamber. Open-type reaction chambers are designed to facilitate temperature calibration.…”

Section: Introductionmentioning

confidence: 99%

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A micro circulating PCR chip using a suction-type membrane for fluidic transport

Chien

Wang

Hsieh

et al. 2008

Biomed Microdevices

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A new micromachined circulating polymerase chain reaction (PCR) chip is reported in this study. A novel liquid transportation mechanism utilizing a suction-type membrane and three microvalves were used to create a new microfluidic control module to rapidly transport the DNA samples and PCR reagents around three bio-reactors operating at three different temperatures. When operating at a membrane actuation frequency of 14.29 Hz and a pressure of 5 psi, the sample flow rate in the microfluidic control module can be as high as 18 microL/s. In addition, an array-type microheater was adopted to improve the temperature uniformity in the reaction chambers. Open-type reaction chambers were designed to facilitate temperature calibration. Experimental data from infrared images showed that the percentage of area inside the reaction chamber with a thermal variation of less than 1 degrees C was over 90% for a denaturing temperature of 94 degrees C. Three array-type heaters and temperature sensors were integrated into this new circulating PCR chip to modulate three specific operating temperatures for the denaturing, annealing, and extension steps of a PCR process. With this approach, the cycle numbers and reaction times of the three separate reaction steps can be individually adjusted. To verify the performance of this circulating PCR chip, a PCR process to amplify a detection gene (150 base pairs) associated with the hepatitis C virus was performed. Experimental results showed that DNA samples with concentrations ranging from 10(5) to 10(2)copies/microL can be successfully amplified. Therefore, this new circulating PCR chip may provide a useful platform for genetic identification and molecular diagnosis.

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Circulating polymerase chain reaction chips utilizing multiple-membrane activation

Cited by 17 publications

References 33 publications

Clockwork PCR Including Sample Preparation

Clockwork PCR Including Sample Preparation

Sample Pretreatment and Nucleic Acid-Based Detection for Fast Diagnosis Utilizing Microfluidic Systems

A micro circulating PCR chip using a suction-type membrane for fluidic transport

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