Circulating pemphigus IgG in families of patients with pemphigus: Comparison of indirect immunofluorescence, direct immunofluorescence, and immunoblotting

Brandsen, Renate; Frušić-Zlotkin, Marina; Lyubimov, Helena; Yunes, Flory; Michel, Beno; Tamir, A.; Milner, Yoram; Brenner, Sarah

doi:10.1016/s0190-9622(97)70324-4

Cited by 62 publications

(51 citation statements)

References 62 publications

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“…In fact, these non-specific antibodies, which can only be assayed by IIF and which are detectable in several disorders such as infectious (bacterial or mycotic) diseases, burns, drug reactions, neoplasms, bullous and cicatricial pemphigoid, do not bind in vivo [15]. The negativity of DIF and of immunoblotting would confirm this hypothesis.…”

Section: Discussionmentioning

confidence: 99%

In vivo Enalapril-Induced Acantholysis

Schiavo

Guerrera²,

Cozzani³

et al. 1999

Dermatology

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Enalapril is a widely used antihypertensive drug with a very powerful in vitro acantholytic effect. It has been known to potentially induce pemphigus in genetically predisposed subjects. The action mechanism is complex and still only partially understood. We describe the case of a 66-year-old man, affected with intermediary basal cell carcinoma, in whom the histological examination showed suprabasal acantholytic clefts in the perilesional epidermis. Surprisingly a second biopsy taken from the apparently healthy skin of his back confirmed the presence of acantholytic changes. Clinical signs of pemphigus were absent. The patient’s history did not reveal any relevant data but a mild arterial hypertension that had been treated for 1 year with 10 mg enalapril. Taking into account the patient’s history (enalapril long-term administration), the absence of any bullous or erosive lesions and the histological findings, a diagnosis was made of in vivo enalapril-induced acantholysis.

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Section: Discussionmentioning

confidence: 99%

In vivo Enalapril-Induced Acantholysis

Schiavo

Guerrera²,

Cozzani³

et al. 1999

Dermatology

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“…Development of PV is further proposed to require epitope spreading from Dsg3 and Dsg1 to keratinocyte cholinergic receptors. Consistent with this model, Brandsen et al (20) have detected anti-Dsg3 antibodies, along with positive direct immunofluorescence of skin, in apparently healthy first-degree relatives of PV patients (20).…”

Section: A Unifying Hypothesismentioning

confidence: 77%

Pemphigus vulgaris: the other half of the story

Kalish

2000

J. Clin. Invest.

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“…In the literature, the healthy relatives of pemphigus patients have been reported to produce low titers of Dsg3-specific autoantibodies [25, 26, 27, 28]. Warren et al [29]have recently reported in an interesting paper that many normal subjects living in an endemic area of fogo selvagem (pemphigus foliaceus) had antibodies against Dsg1, and they demonstrated that these antibodies were present several years before the induction of real pemphigus.…”

Section: Discussionmentioning

confidence: 99%

Antidesmoglein Autoantibodies in Silicosis Patients with No Bullous Diseases

Ueki¹,

Kohda²,

Nobutoh³

et al. 2001

Dermatology

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Background: Pemphigus is an autoimmune bullous disease characterized by the presence of antidesmoglein autoantibodies. However, the mechanism of its autoantibody production remains unknown. In previous reports, we have described rare cases of pemphigus and pemphigoid associated with silicosis. It is well known that during long-term silicosis, some autoimmune diseases, such as systemic sclerosis, systemic lupus erythematosus or rheumatoid arthritis, can occur. Objective: The aim of this study was to explore the presence of pemphigus or pemphigoid autoantibodies in silicosis patients without clinical bullous diseases or collagen diseases. Method: The presence of pemphigus antibodies was examined in 54 silicosis patients with no associated bullous diseases, using immunofluorescence, the enzyme-linked immunosorbent assay (ELISA) for desmoglein 1 and 3, and immunoblotting methods. In the antibody-positive cases, HLA genotyping of peripheral lymphocytes was performed with PCR-RFLP. Results: Seven out of the 54 patients were found to be positive for pemphigus antibodies and 1 for bullous pemphigoid by immunofluorescence. In addition, by ELISA, 6 patients were found to be positive against the desmoglein 1 antigen, 2 against the desmoglein 3 antigen and 2 against both desmoglein 1 and desmoglein 3. Conclusion: The results of the present study strongly suggest the occurrence of pemphigus and pemphigoid autoantibodies in patients with silicosis. It remains unclear whether such patients will develop an autoimmune bullous disease in the future. Accordingly, long-term follow-up of antibody-positive patients is required.

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Circulating pemphigus IgG in families of patients with pemphigus: Comparison of indirect immunofluorescence, direct immunofluorescence, and immunoblotting

Cited by 62 publications

References 62 publications

In vivo Enalapril-Induced Acantholysis

In vivo Enalapril-Induced Acantholysis

Pemphigus vulgaris: the other half of the story

Antidesmoglein Autoantibodies in Silicosis Patients with No Bullous Diseases

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