Circulating non-coding RNAs in chronic kidney disease and its complications

Zonneveld, Anton Jan van; Qiao, Zhao; Rotmans, Joris I.; Bijkerk, Roel

doi:10.1038/s41581-023-00725-w

Cited by 16 publications

(12 citation statements)

References 171 publications

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“…A limitation of our study is the use of a total RNA isolation procedure and as such we were not able to investigate if the sncRNAs are carried by extracellular vesicles or are associated with carriers such as HDL and Ago-2 [2]. The use of plasma for RNA isolation is another limitation.…”

Section: Discussionmentioning

confidence: 99%

“…MicroRNAs are involved in the post-transcriptional regulation of gene expression and are known to be involved in crosstalk between (distant) cells and tissues [2]. As such, there is an ever growing interest to study their role in disease development and as biomarkers of disease and disease progression.…”

Section: Associations Between Kidney Function and Sncrnas Mirnasmentioning

confidence: 99%

“…Circulating small non-coding RNAs (sncRNA) are mostly contained in extracellular vesicles or are bound to RNA-binding proteins and lipoproteins and originate from various tissues including tissues important in kidney function [2]. Whereas previously it was thought that sncRNAs merely reflect degradation by-products, it has now been shown that they exert important functions in cell-to-cell communication, the regulation of gene expression, splicing and ribosomal RNA maturation [2].…”

Section: Introductionmentioning

confidence: 99%

“…Circulating small non-coding RNAs (sncRNA) are mostly contained in extracellular vesicles or are bound to RNA-binding proteins and lipoproteins and originate from various tissues including tissues important in kidney function [5]. The composition of sncRNAs in the circulation is dependent on tissue homeostasis and can be altered by disease development.…”

Section: Introductionmentioning

confidence: 99%

“…The composition of sncRNAs in the circulation is dependent on tissue homeostasis and can be altered by disease development. Whereas previously it was thought that sncRNAs merely reflect degradation by-products, it has now been shown that they exert important functions in cell-to-cell communication, the regulation of gene expression, splicing and ribosomal RNA maturation [5]. Irrespective of the mechanism involved, sncRNAs may have an impact on the development and progression of DKD when deregulated.…”

Section: Introductionmentioning

confidence: 99%

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Small RNA sequencing reveals snoRNAs and piRNA-019825 as novel players in diabetic kidney disease

Hart

Bouland

Klerk

et al. 2022

Preprint

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Aims/hypothesis Micro- and macrovascular complications are common among persons with type 2 diabetes. Interest into the potential of circulating small non-coding RNAs (sRNAs) as biomarkers or drivers of the development of diabetic complications is growing. In this study we investigated if circulating sRNAs levels associate with prevalent chronic kidney disease (CKD) in persons with type 2 diabetes. Methods Plasma sRNAs levels were determined using sRNA-seq, allowing detection of miRNAs, snoRNAs, piRNAs, tRNA-fragments and various other sRNA classes, in persons with type 2 diabetes, with CKD (n=69) or without CKD (n=405). Multiple regression analyses were used to test for associations between the sRNAs and primary endpoint CKD. In secondary analyses, we also tested the association with eGFR and albuminuria (UACR). Results In total, twelve sRNA were associated with prevalent CKD (logFC = -0.32 to -1.28, all P≤6.24x10-4). Although miRNAs represent the majority of the sRNAs measured (64%) only four miRNA were significantly associated with prevalent CKD. Interestingly, the majority of the significant sRNA belonged to the snoRNAs (58%). Similar results were observed for eGFR and UACR. Only one of the twelve sRNAs showed its highest expression in kidney whereas several others showed high expression in liver or colon. Conclusions/interpretation Small RNAs present in the circulation associate with CKD in persons with type 2 diabetes. Further studies are warranted to elucidate the biological role of sRNA in diabetic CKD and in particular snoRNAs. High expression of these circulating sRNAs in tissues other than kidney suggest a role in inter-organ communication.

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Section: Discussionmentioning

confidence: 99%

Section: Associations Between Kidney Function and Sncrnas Mirnasmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Circulating small non-coding RNAs (sncRNA) are mostly contained in extracellular vesicles or are bound to RNA-binding proteins and lipoproteins and originate from various tissues including tissues important in kidney function [5]. The composition of sncRNAs in the circulation is dependent on tissue homeostasis and can be altered by disease development.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Small RNA sequencing reveals snoRNAs and piRNA-019825 as novel players in diabetic kidney disease

Hart

Bouland

Klerk

et al. 2022

Preprint

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Small RNA sequencing reveals snoRNAs and piRNA-019825 as novel players in diabetic kidney disease

’t Hart,

de Klerk,

Bouland

et al. 2024

Endocrine

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Introduction Micro- and macrovascular complications are common among persons with type 2 diabetes. Recently there has been growing interest to investigate the potential of circulating small non-coding RNAs (sncRNAs) as contributors to the development of diabetic complications. In this study we investigate to what extent circulating sncRNAs levels associate with prevalent diabetic kidney disease (DKD) in persons with type 2 diabetes. Methods Plasma sncRNAs levels were determined using small RNA-seq, allowing detection of miRNAs, snoRNAs, piRNAs, tRNA fragments, and various other sncRNA classes. We tested for differentially expressed sncRNAs in persons with type 2 diabetes, with DKD (n = 69) or without DKD (n = 405). In secondary analyses, we also tested the association with eGFR, albuminuria (UACR), and the plasma proteome. Results In total seven sncRNAs were negatively associated with prevalent DKD (all PFDR ≤ 0.05). Including one microRNA (miR-143-5p), five snoRNAs (U8, SNORD118, SNORD24, SNORD107, SNORD87) and a piRNA (piR-019825 | DQ597218). Proteomic analyses showed that the seven sncRNAs, and especially the piRNA piR-019825, were associated with plasma levels of 24 proteins of which several have known associations with kidney function including TNF sR-I (TNFRFS1A), DAN (NBL1) and cystatin C (CST3). Conclusion We have identified novel small non-coding RNAs, primarily from classes other than microRNAs, that are associated with diabetic kidney disease. Our results show that the involvement of small non-coding RNAs in DKD goes beyond the already known microRNAs and also involves other classes of sncRNA, in particular snoRNAs and the piRNA piR-019825, that have never been studied before in relation to kidney function.

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Mitochondrial iron regulation as an emerging target in ischemia/reperfusion injury during kidney transplantation

Wang

et al. 2023

Biochemical Pharmacology

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Circulating non-coding RNAs in chronic kidney disease and its complications

Cited by 16 publications

References 171 publications

Small RNA sequencing reveals snoRNAs and piRNA-019825 as novel players in diabetic kidney disease

Small RNA sequencing reveals snoRNAs and piRNA-019825 as novel players in diabetic kidney disease

Small RNA sequencing reveals snoRNAs and piRNA-019825 as novel players in diabetic kidney disease

Mitochondrial iron regulation as an emerging target in ischemia/reperfusion injury during kidney transplantation

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