Circulating MiRNAs as biomarkers of gait speed responses to aerobic exercise training in obese older adults

Zhang, Tan; Brinkley, Tina E.; Liu, Keqin; Feng, Xin; Marsh, Anthony P.; Kritchevsky, Stephen B.; Zhou, Xiaobo; Nicklas, Barbara J.

doi:10.18632/aging.101199

Cited by 23 publications

(16 citation statements)

References 81 publications

(68 reference statements)

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“…Specifically, circulating miR-1, miR-133a, miR-133b, miR-139, miR-143, miR-181b, miR-206, miR-208b, miR-214, miR-223, miR-330, miR-338, miR-485, miR-509, miR-517a, miR-518f, miR-520f, miR-522, miR-553, and miR-888 were found increased, while miR-30b, miR-106a, miR-146a, miR-151, miR-221, miR-652 were decreased after an acute exercise (Banzet et al, 2013;Nielsen et al, 2014;Cui et al, 2016;Silva et al, 2017). , and miR-766 were decreased after a chronic exercise (Baggish et al, 2011(Baggish et al, , 2014Nielsen et al, 2014;Zhang et al, 2017b). Circulating miR-21, miR-146a, miR-221, miR-222 were increased in response to both acute and chronic exercise (Baggish et al, 2011).…”

Section: Discussionmentioning

confidence: 97%

Circulating microRNAs in Response to Exercise Training in Healthy Adults

Zhou

Shi

et al. 2020

Front. Genet.

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Circulating microRNAs (miRNAs, miRs) have great potential as cardiac biomarkers and they are also being explored for their roles in intercellular communication and gene expression regulation. The analysis of circulating miRNAs in response to exercise would provide a deeper understanding of the molecular response to physical activity and valuable information for clinical practice. Here, eight male college students were recruited to participate in cardiopulmonary exercise testing (CPET) and 1 h acute exercise training (AET). Blood samples were collected and serum miRNAs involved in angiogenesis, inflammation and enriched in muscle and/or cardiac tissues were analyzed before and after cardiopulmonary exercise and acute exercise. The miRNAs we detected were miR-1, miR-20a, miR-21, miR-126, miR-133a, miR-133b, miR-146, miR155, miR-208a, miR-208b, miR-210, miR-221, miR-222, miR-328, miR-378, miR-499, and miR-940. We found that serum miR-20a was decreased significantly after CPET and serum miR-21 was increased after AET. In addition, no robust correlation was identified between the changes of these miRNAs and makers of cardiac function and exercise capacity, which indicates a distinct adaptation of these miRNAs to exercise. Future studies are highly needed to define the potential use of these circulating miRNAs as useful biomarkers of exercise training, and disclose the biological function of circulating miRNAs as physiological mediators of exercise-induced cardiovascular adaptation.

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Section: Discussionmentioning

confidence: 97%

Circulating microRNAs in Response to Exercise Training in Healthy Adults

Zhou

Shi

et al. 2020

Front. Genet.

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“…They may act as tumour suppressors or oncogenes by regulating gene transcription [17,18]. It has been shown that circulating miRNAs may act as biomarkers of gait speed in obese adults [19] or have a prognostic role in cancer [20]. More recently, several articles confirmed that plasma miRNAs could be biomarkers of RCC, such as miR-144À3p and miR-210 [21,22].…”

Section: Introductionmentioning

confidence: 99%

MiR-765 functions as a tumour suppressor and eliminates lipids in clear cell renal cell carcinoma by downregulating PLP2

et al. 2020

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A B S T R A C TBackground: Lipid accumulation has been highlighted in cancer development and progression, but the exact mechanism remains unclear in renal cell carcinoma (RCC). MicroRNAs (miRNAs) have been confirmed to participate in the pathological processes of cancers, including tumour occurrence and inhibition. However, the role and mechanism of miR-765 have not been elucidated in clear cell renal cell carcinoma (ccRCC). Methods: Using The Cancer Genome Atlas (TCGA) database and qRT-PCR, we investigated differences in miR-765 and proteolipid protein 2 (PLP2) expression, as well as their clinical relevance. To investigate the function of miR-765 and PLP2 in ccRCC, we performed in vitro and in vivo experiments to explore their biological functions in ccRCC. Findings: In this study, we showed that miR-765 was upregulated in the plasma of ccRCC patients after tumour resection. Consistently, ccRCC tissues had low expression of miR-765 when compared with corresponding non-cancerous tissues. Overexpression of miR-765 suppressed cell proliferation and metastasis in vitro and in vivo. Mechanistic studies demonstrated that PLP2 was a direct target gene of miR-765. PLP2 was highly expressed in ccRCC tissues, and high PLP2 levels were positively correlated with higher tumour stage and grade and poor prognosis. PLP2 expression was negatively correlated with the miR-765 level in patient samples. We further showed that PLP2 restrained the cell metastasis and proliferation induced by miR-765 and reduced the lipid-eliminating effects of miR-765 in renal cancer cells. Interpretation: Our findings suggest that miR-765 may function as a tumour suppressor and eliminate lipids in clear cell renal cell carcinoma by targeting PLP2. EBioMedicine journal homepage: www.elsevier.com/locate/ebiom that miR-765 inhibits osteosarcoma [23] and tongue squamous cell carcinoma [24]. Nevertheless, to the best of our knowledge, there is still no relevant research about the role of miR-765 in ccRCC. We found that the plasma level of miR-765 was upregulated after tumour resection in ccRCC patients and that ccRCC tissues had a low level of miR-765 compared with corresponding non-ccerous tissues. The present study systematically describes the function and role of miR-765 in ccRCC. Functionally, miR-765 inhibited RCC cell proliferation, migration and invasion in vitro and in vivo. Mechanistically, miR-765 serves as a tumour suppressor and eliminates lipids by downregulating PLP2 in ccRCC. Our findings reveal that the miR-765-PLP2 axis may be a biomarker and a therapeutic target for ccRCC. Materials and methods Patient samples and cellsThirty-six clear cell renal cell carcinoma (ccRCC) patient surgical specimens, 18 non-ccRCC (nccRCC) patient surgical specimens and 18 blood plasma samples (preoperative and operational day 7 without chemotherapy or radiotherapy for ccRCC) were obtained from

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“…Several miRs have been proposed as potential biomarkers of sarcopenia. Among them, circulating miR-21, miR-134a, miR-133 and miR-206 have diagnostic and/or prognostic potential due to their correlations with physical capacity [ 20 ]. However, their associations with indexes of sarcopenia in respiratory diseases are not known.…”

Section: Introductionmentioning

confidence: 99%

Circulating Biomarkers of Accelerated Sarcopenia in Respiratory Diseases

Qaisar

Karim

Muhammad

et al. 2020

Biology

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Skeletal muscle dysfunction is a critical finding in many respiratory diseases. However, a definitive biomarker to assess muscle decline in respiratory diseases is not known. We analyzed the association of plasma levels of glycoprotein Dickkopf-3 (Dkk-3), c-terminal agrin fragment-22 (CAF22) and microRNAs miR-21, miR-134a, miR-133 and miR-206 with hand-grip strength (HGS) and appendicular skeletal mass index (ASMI) in male, 54–73-year-old patients with chronic obstructive pulmonary diseases (COPD), asthma or pulmonary TB (n = 83–101/group). Patients with respiratory diseases showed a reduction in HGS and gait speed, while a reduction in ASMI was only found in patients with pulmonary TB. Among the sarcopenia indexes, HGS showed the strongest correlation with plasma CAF22, miR-21 and miR-206 levels while ASMI showed the strongest correlation with Dkk-3 and miR-133 in respiratory diseases. We found a modest-to-significant increase in the plasma markers of inflammation, oxidative stress and muscle damage, which had varying degrees of correlations with Dkk-3, CAF22 and selected micro RNAs (miRs) in respiratory diseases. Taken together, our data show that plasma levels of Dkk-3, CAF22 and selected miRs can be useful tools to assess accelerated sarcopenia phenotype in the elderly with respiratory diseases.

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Circulating MiRNAs as biomarkers of gait speed responses to aerobic exercise training in obese older adults

Cited by 23 publications

References 81 publications

Circulating microRNAs in Response to Exercise Training in Healthy Adults

Circulating microRNAs in Response to Exercise Training in Healthy Adults

MiR-765 functions as a tumour suppressor and eliminates lipids in clear cell renal cell carcinoma by downregulating PLP2

Circulating Biomarkers of Accelerated Sarcopenia in Respiratory Diseases

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