Circulating miRNA Expression Profiling and Target Prediction in Patients Receiving Dexmedetomidine

Abstract

Background/Aims: Circulating miRNAs could serve as biomarkers for diagnosis or prognosis of heart diseases and cerebrovascular diseases. Dexmedetomidine has protective effects in various organs. The effects of dexmedetomidine on circulating miRNAs remain unknown. Here, we investigated differentially expressed miRNA and to predict the target genes of the miRNA in patients receiving dexmedetomidine. Methods: The expression levels of circulating miRNAs of 3 patients were determined through high through-put miRNA sequencing technology. Target genes of the identified differentially expressed miRNAs were predicted using TargetScan 7.1 and miRDB v.5. Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to conduct functional annotation and pathway enrichment analysis of target genes respectively. Results: Twelve differentially expressed miRNAs were identified. Five miRNAs were upregulated (hsa-miR-4508, hsa-miR-novel-chr8_87373, hsa-miR-30a-3p, hsa-miR-novel-chr16_26099, hsa-miR-4306) and seven miRNAs (hsa-miR-744-5p, hsa-miR-320a, hsa-miR-novel-chr9_90035, hsa-miR-101-3p, hsa-miR-150-5p, hsa-miR-342-3p, and hsa-miR-140-3p) were downregulated after administration of dexmedetomidine in the subjects. The target genes and pathways related to the differentially expressed miRNAs were predicted and analyzed. Conclusion: The differentially expressed miRNAs may be involved in the mechanisms of action of dexmedetomidine. Specific miRNAs, such as hsa-miR-101-3p, hsa-miR-150-5p and hsa-miR-140-3p, are new potential targets for further functional studies of dexmedetomidine.

Plasma-Derived Exosomal hsa-miR-4488 and hsa-miR-1228-5p: Novel Biomarkers for Dermatomyositis-Associated Interstitial Lung Disease with Anti-Melanoma Differentiation-Associated Protein 5 Antibody-Positive Subset

“…Furthermore, it was indicated that hsa-miR-4508 was a significant highly expressed miRNA in exosomes. It has been reported that circulating hsa-miR-4508 was significantly upregulated in acute myocardial infarction [ 37 ] and patients receiving dexmedetomidine [ 38 ]. However, its functions remain elusive.…”

Comparative rna‐seq analysis of region‐specific miRNA expression in the epididymis of cattleyak

“…MicroRNAs (miRNAs) are one family of small non‐coding RNAs that regulate gene expression post‐transcriptionally, generally by binding to specific motifs in the 3’‑untranslated regions (3’UTRs) of target genes Bartel, (2009). MicroRNAs coordinate diverse biological events, including differentiation, apoptosis, proliferation, reproduction, stress response, metabolism, immune response and stem cell maintenance (Beitzinger et al., 2007; Gangaraju & Lin, 2009; Huang et al., 2011; Zhang et al., 2013; Junhua et al., 2017; Yang, Chen, et al., 2018). MicroRNAs have been shown to play an important role in the acquisition and maintenance of male fertility.…”

Analgesic effect of dexmedetomidine in rats after chronic constriction injury by mediating microRNA‐101 expression and the E2F2–TLR4–NF‐κB axis

“…Recently, microRNAs (miRNAs) have been revealed to play vital roles in modulating pain transmission in animal experiments and clinical studies (Dai et al., 2019). For instance, hsa‐miR‐101‐3p has been identified as one of the seven miRNAs that were reduced after delivery of Dex to the subjects (Yang et al., 2018). The neuroprotective role of miR‐101 has been underscored in Alzheimer's disease (Vilardo, Barbato, Ciotti, Cogoni, & Ruberti, 2010).…”