Circulating MiRNA-195-5p and -451a in Diabetic Patients with Transient and Acute Ischemic Stroke in the Emergency Department

Abstract

(1) Background: Circulating micro-RNAs (miRNAs) modulate the expression of molecules in diabetes. We evaluated the expression of serum miRNA-195-5p and -451a in diabetic patients with ischemic stroke and correlated them with two markers of brain tissue integrity. (2) Methods: Seventy-eight subjects with acute ischemic stroke (AIS) or transient ischemic attack (TIA) (40 with diabetes) were enrolled. Serum miRNA levels, brain-derived neurotrophic factor (BDNF) and vascular endothelial growth factor A (VEGF-A) were assessed at admission and 24 and 72 h after a post-ischemic stroke, and were compared to 20 controls. (3) Results: Both circulating miRNAs were two-fold up-regulated in diabetic AIS and TIA patients compared to non-diabetics. Their levels progressively decreased at 24 and 72 h in both AIS and TIA patients. Interestingly, in the non-diabetic TIA group, both circulating miRNAs, although higher than the controls, tended to achieve a complete decay after 72 h. Furthermore, miRNA-195-5p and miRNA-451a levels inversely correlated with both BDNF and VEGF-A serum levels. (4) Conclusions: These data show a different profile of both micro-RNAs in diabetic versus non-diabetic patients after acute ischemic stroke, suggesting their pivotal role in cerebrovascular ischemic attack.

Bioinformatics Analysis for Identifying Micro-RNAs, Long Noncoding RNAs, Transcription Factors, Immune Genes Regulatory Networks, and Potential Therapeutic Agents for Diabetic Cardiomyopathy Using an Integrated Bioinformatics Analysis

“…They also contribute to the development of DCM by regulating oxidative stress, in ammation, cardiomyocyte apoptosis, and mitochondrial function 2 . Previous study has reported that miRNA-195 inhibitors can reduce diabetic myocardial hypertrophy and improve cardiac function by reducing oxidative damage, inhibiting cell apoptosis, and promoting angiogenesis 30 . Moreover, miRNAs are involved in the regulation of multiple biological functions in the mitochondria by binding to their target genes 31 .…”

Genetics of macrovascular complications in type 2 diabetes

“…According to the results of recent research, miR-451a and miR-195-5p were found to be upregulated in diabetic patients after a transient ischemic attack in comparison to control patients and non-diabetic patients after a transient ischemic attack[ 106 ]. The earlier study also demonstrated an increased expression level of miR-451a in the serum of subjects with ischemic stroke[ 107 ].…”

Corneal nerve loss in patients with TIA and acute ischemic stroke in relation to circulating markers of inflammation and vascular integrity

“…Elevated levels of matrix metalloproteinase (MMP)-9 are associated with infarct growth and hemorrhagic transformation in patients admitted with acute ischemic stroke [8], whilst lower levels are associated with a better pre-stroke collateral status [9]. Whilst serum vascular endothelial growth factor (VEGF) levels were lower in patients with TIA or AIS [10], elevated VEGF levels were associated with larger infarct volume, post stroke cognitive impairment [11] and poorer outcome [12]. Although, a recent systematic review and meta-analysis has shown no association between serum VEGF levels and outcomes in ischemic stroke [13].…”