Circulating miRNA-1290 as a potential biomarker for response to chemoradiotherapy and prognosis of patients with advanced oral squamous cell carcinoma: A single-center retrospective study

Abstract

MicroRNAs are a class of small, endogenous, noncoding 18-to 24-nucleotide-long RNAs that can regulate multiple processes related to cancer progression. However, their clinical value in patients with oral squamous cell carcinoma has not yet been fully explored. Therefore, the aim of this study was to investigate the clinical significance of circulating microRNAs in oral squamous cell carcinoma patients. The expression levels of circulating miR-1246 and miR-1290 in healthy volunteers and oral squamous cell carcinoma patients were examined by quantitative real-time polymerase chain reaction. The expression levels of both microRNAs in the radioresistant oral squamous cell carcinoma cell line (SAS-R) and the parent cell line (SAS) and in the conditioned medium obtained from these cell lines were also examined by quantitative real-time polymerase chain reaction. In addition, the correlations between circulating microRNA status and various clinicopathological features in 55 oral squamous cell carcinoma patients with locally advanced oral squamous cell carcinoma who underwent surgery following 5-fluorouracil-based chemoradiotherapy were examined. The expression level of miR-1290 was significantly lower in the plasma of oral squamous cell carcinoma patients than in that of healthy volunteers (p \ 0.01). The expression levels of microRNAs in the conditioned medium and in the cells varied from cell to cell. In the clinicopathological analyses, the frequency of patients with low miR-1290 levels was significantly higher among cases with lower pathological differentiation and among those with a poor pathological response for preoperative chemoradiotherapy (p = 0.030 each). Furthermore, Cox regression analysis based on the 5-year overall survival and disease-free survival revealed that miR-1290 status was a significant prognostic factor for patients with oral squamous cell carcinoma (hazard ratio = 0.169, p = 0.008, and hazard ratio = 0.186, p = 0.008, respectively). Circulating miR-1290 status could be a valuable biomarker for predicting the clinical response to chemoradiotherapy as well as overall survival in patients with oral squamous cell carcinoma.

Integrated Bioinformatics Analysis of mRNAs and miRNAs Identified Potential Biomarkers of Oral Squamous Cell Carcinoma

“…Furthermore, research observations indicated that, miR-1290 can behave as non-invasive biomarkers that could be used as early detection, prognostic and diagnostic biomarker for broad spectrum of cancers (Mo et al, 2015;Kobayashi et al, 2018). In case of oral cancer, Nakashima et al found that miR-1290 expression level was significantly lower in the plasma of oral squamous cell carcinoma patients than in that of healthy, however, circulating miR-1290 status suggested as useful biomarker to predict the clinical response to chemoradiotherapy as well as overall survival in oral squamous cell carcinoma patients (Nakashima et al, 2019). As we found that most of hub genes (including CD44, ACLY, ACTR3, STAT1, LAMC2 and YWHAZ) were potentially targeted by has-miR-5580-3p, it indicates that this miRNA might play a key role in OSCC.…”

Circulating MiR-1290 as a potential diagnostic and disease monitoring biomarker of human gastrointestinal tumors

“…Ling Ye et al discovered that tissue miR-1290 expression levels were positively correlated with the dMMR status and predicted the prognosis of patients with CRC receiving 5-FU treatment [ 32 ]. The circulating miR-1290 level was also a potential biomarker for the response of patients with advanced oral squamous cell cancer to 5-FU-based chemoradiotherapy [ 33 ]. Therefore, the relationship between circulating miR-1290 expression and chemotherapy resistance in patients with gastrointestinal tumors is worthy further study.…”

Circulating MiR-1290 as a Potential Diagnostic and Disease Monitoring Biomarker of Human Gastrointestinal Tumors

“…Circulating miR-1290 was also a potential biomarker for response to 5-FU-based chemoradiotherapy of patients with advanced oral squamous cell cancer [30]. Therefore, the relationship between circulating miR-1290 expression and chemotherapy resistance in gastrointestinal tumors is worthy of study.…”