Circulating MIR148A associates with sensitivity to adiponectin levels in human metabolic surgery for weight loss

Abstract

ObjectiveWe sought to discover secreted biomarkers to monitor the recovery of physiological adiponectin levels with metabolic surgery, focusing on epigenetic changes that might predict adiponectin function.DesignWe conducted a prospective observational study of patients undergoing metabolic surgery by Roux-en-Y Gastric Bypass (RYGB) for weight loss in a single center (IRB GHS # 1207-27).MethodsAll patients (n = 33; 27 females; 6 males) signed informed consent. Metabolites, adiponectin and MIR148A were measured in fasting plasma. We followed MIQE for transcript profiles.ResultsPatients lost on average 47 ± 12% excess BMI (%EBMI) after 12 weeks. Adiponectin pre, post or delta (post minus pre) did not correlate with %EBMIL. A decrease in adiponectin following weight loss surgery was observed in a subset of patients, chi-square test of independence rejects the null hypotheses that the liver DNA methyltransferase 1 (DNMT1) and delta adiponectin are independent (chi-square statistics χ2 = 6.9205, P = 0.00852, n = 33), as well as MIR148A and delta adiponectin are independent (chi-square statistics χ2 = 9.6823, P = 0.00186, n = 33). The presence of plasma MIR148A allows identification of patients that appear to be adiponectin insensitive at baseline.ConclusionWe combined the presence of plasma MIR148A, the concentration of total adiponectin and the expression of DNA methyltransferase 1 (DNMT1) in liver biopsy tissue to identify patients with non-physiological adiponectin. Weight loss and physical activity interventions complemented with the new method presented here could serve to monitor the physiological levels of adiponectin, thought to be important for long-term weight loss maintenance.

Efecto de la ingesta crónica de fructosa sobre los niveles plasmáticos de microRNAs relacionados con la adipogénesis en ratas Wistar

“…Por otro lado, en sujetos con síndrome metabólico presentaron niveles elevados del miR-148a-5p [96] y en sujetos con obesidad mórbida [148]. También se encontró que niveles elevados del miR-148a-5p en el plasma de sujetos obesos se relacionó con la disminución de la expresión de DNA metiltransferasa 1 (DNMT1), regulador de los niveles de adiponectina, lo cual se relacionó con estados insensibles a la adiponectina [149]. Por lo tanto, la evidencia indica que el miR-148a-5p juega un papel importante en el desarrollo de alteraciones metabólicas como la obesidad y RI y se sabe que la alta ingesta de fructosa es un potente inductor de estás enfermedad.…”