Circulating miR-92a expression level in patients with essential hypertension: a potential marker of atherosclerosis

Abstract

MicroRNAs (miRs) are key posttranscriptional regulators of gene expression in all eukaryotic cells and have a vital role in the evolution of hypertension and cardiovascular remodelling and, therefore, have emerged as potential biomarkers for cardiovascular disease. We assessed 240 participants, including 60 healthy volunteers with normal carotid intima-media thickness (nCIMT), 60 healthy volunteers with increased CIMT (iCIMT), 60 hypertensive patients with nCIMT and 60 hypertensive patients with iCIMT. All patients underwent measurements of CIMT, carotid-femoral pulse wave velocity (cfPWV) and ambulatory blood pressure (BP) monitoring. Plasma miR-92a expression was quantified by real-time reverse transcription PCR. Correlations between miR-92a expression and BP parameters, CIMT and cfPWV were assessed using the Spearman correlation coefficient. We observed the lowest miR-92a expression (24.59±1.30 vs 27.76±2.13 vs 29.29±1.89 vs 33.76±2.08; P<0.001) in healthy controls with nCIMT, followed by healthy controls with iCIMT, then hypertensive patients with nCIMT and the highest expression in hypertensive patients with iCIMT. Additionally, MiR-92a levels showed a significant positive correlation with 24-h mean systolic BP (r=0.807, P<0.001), 24-h mean diastolic BP (r=0.649, P<0.001), 24-h mean pulse pressure (PP) (r=0.697, P<0.001), 24-h daytime PP (r=0.654, P<0.001), 24-h nighttime PP (r=0.573, P<0.001), CIMT (r=0.571, P<0.001) and cfPWV (r=0.601, P<0.001). Our data present significant evidence that circulating miR-92a represents a potential noninvasive atherosclerosis marker in essential hypertensive patients.

Exosomal miR-92a Concentration in the Serum of Shift Workers

“…Several miRNAs have proven to play a major role in BAT, such as controlling brown adipocytes differentiation [36]. Among these miRNAs, miR-92a has been involved in several metabolic processes, such as glucose homeostasis [24], regulation of intracellular cholesterol levels [37], and essential hypertension [38]. Furthermore, Chen and collaborators demonstrated that serum levels of exosomal miR-92a were inversely correlated with human BAT activity, confirming the data by FDG-PET/CT [39].…”

Early Programming of Adult Systemic Essential Hypertension

“…An aberrant profile expression of miRNAs has been linked to a series of diseases, including hypertension and miRNA expression in the circulation shows changes in hypertensive subjects. Among the circulating levels of miRNAs that have been found to be elevated in hypertensive patients, the following can be mentioned: miR-221 and miR-222 [158], miR-92a [159,160], miR-21 and miR-1 [161] miR-516b, miR-600, miR-605, miR-623, and let-7e [162] and miR-506-3p [163]. Among the miRNAs that have been reported to be reduced in hypertensive patients, there is miR-143, miR-145, miR-133 [161], miR-18b, miR-30d, miR-296-5p, miR-324-3p, miR-486-5p, miR-518b, miR-1236, and miR-1227 [162].…”

MicroRNA in ischemic stroke

“…Уровень мРНК-92a в плазме крови был выше у пациентов с АГ (независимо от толщины комплекса интима-медиа сонной артерии), чем у здоровых [50]. Уровень МРНК-92a был положительно связан со скоростью пульсовой волны в сонной артерии и уровнем АД.…”