Circulating miR-29a, Among Other Up-Regulated MicroRNAs, Is the Only Biomarker for Both Hypertrophy and Fibrosis in Patients With Hypertrophic Cardiomyopathy

Roncarati, Roberta; Anselmi, Chiara; Losi, Maria Angela; Papa, Laura; Cavarretta, Elena; Martins, Paula da Costa; Contaldi, Carla; Jotti, Gloria Saccani; Franzone, Anna; Galastri, Laura; Latronico, Michael V.G.; Imbriaco, Massimo; Esposito, Giovanni; Windt, León J. De; Betocchi, Sandro; Condorelli, Gianluigi

doi:10.1016/j.jacc.2013.09.041

Cited by 266 publications

(236 citation statements)

References 40 publications

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“…Overall, however, physiological stimuli and comorbidities seem to exert a modest impact especially on the phenotypic expression of HCM. Future research targeting HCM variability should rather focus on molecular aspects including modifier genes, epigenetic factors, and the role of regulatory systems such as microRNAs, the ubiquitine‐proteasome complex, or nonsense‐mediated RNA decay 78, 79…”

Section: Discussionmentioning

confidence: 99%

Impact of Demographic Features, Lifestyle, and Comorbidities on the Clinical Expression of Hypertrophic Cardiomyopathy

Finocchiaro

Magavern

Sinagra

et al. 2017

JAHA

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Section: Discussionmentioning

confidence: 99%

Impact of Demographic Features, Lifestyle, and Comorbidities on the Clinical Expression of Hypertrophic Cardiomyopathy

Finocchiaro

Magavern

Sinagra

et al. 2017

JAHA

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“…miRNAs are already being used commercially as disease biomarkers in oncology [17]. There is evidence for the use miRNAs as biomarkers in a wide range of diseases including prostate cancer [36], cardiac disease [37], acute kidney injury [38], and Alzheimer's disease [39]. If we could identify miRNAs that could be used as biomarkers for PCOS, the diagnosis and treatment of PCOS would be revolutionized.…”

Section: Discussionmentioning

confidence: 99%

Altered microRNA and gene expression in the follicular fluid of women with polycystic ovary syndrome

Roth

McCallie

Alvero

et al. 2014

J Assist Reprod Genet

156

127

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Purpose To determine if microRNAs are differentially expressed in the follicular fluid of women with PCOS compared to fertile oocyte donors and identify associated altered gene expression. Methods Women undergoing IVF who met Rotterdam criteria for PCOS or who were fertile oocyte donors were recruited from a private IVF center. Individual follicle fluid was collected at the time of oocyte retrieval. MicroRNA analysis was performed using microarray and validated using real-time PCR on additional samples. Potential gene targets were identified and their expression analyzed by real time PCR. Results Microarray profiling of human follicular fluid revealed expression of 235 miRNAs, 29 were differentially expressed between the groups. Using PCR validation, 5 miRNAs (32, 34c, 135a, 18b, and 9) showed significantly increased expression in the PCOS group. Pathway analysis revealed genes involved in insulin regulation and inflammation. Three potential target genes were found to have significantly decreased expression in the PCOS group (interleukin 8, synaptogamin 1, and insulin receptor substrate 2).Conclusions MicroRNAs are differentially expressed in the follicular fluid of women with PCOS when compared to fertile oocyte donors. There is also altered expression of potential target genes associated with the PCOS phenotype.

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“…It is widely accepted that deregulated expression levels of certain miRNAs directly participate in the development and progression of numerous types of human cancer. In addition to its role in cancer, miR-29a has also been demonstrated to be associated with osteoblastic differentiation (15), myogenesis (16), sclerosis (17), fibrosis (18), HIV-1 replication (19), diabetes (20) and Alzheimer's disease (21). In the current study, it was identified that overexpression of miR-29a markedly suppressed the migratory and invasive capacities of gastric cancer cells.…”

Section: Discussionmentioning

confidence: 61%

MicroRNA-29a inhibits cell migration and invasion via targeting Roundabout homolog 1 in gastric cancer cells

Liu

Cai

Sun

et al. 2015

Molecular Medicine Reports

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Abstract. Deregulation of Roundabout homolog 1 (Robo1) has been demonstrated to be associated with several types of human cancer, including gastric cancer. However, the detailed role of Robo1 and its regulatory mechanism in gastric cancer remain largely unclear. In the current study, it was demonstrated that the expression of microRNA (miR)-29a was frequently reduced in gastric cancer tissues, compared with their matched normal adjacent tissues. Similar results were additionally observed in AGS and SGC-7901 human gastric cancer cells. Overexpression of miR-29a led to reduced migration and invasion of AGS cells. To explore the targets of miR-29a in gastric cancer, bioinformatics analysis was conducted and Robo1 was identified as a putative target of miR-29a. Further western blotting and luciferase activity assay data confirmed that miR-29a was able to negatively regulate the protein expression of Robo1, through directly binding to the 3'-untranslated region of Robo1 mRNA in gastric cancer cells. In addition, it was demonstrated that Robo1 was frequently upregulated in gastric cancer tissues compared with their matched adjacent normal tissues, and a significant inverse correlation was identified between miR-29a and Robo1 expression. In addition, knockdown of Robo1 by small interfering RNA markedly inhibited the migratory and invasive capabilities of AGS cells, which the results obtained with overexpression of miR-29a. In conclusion, to the best of our knowledge the current study suggested for the first time, that miR-29a inhibits migration and invasion in part via direct inhibition of Robo1 in gastric cancer cells. Therefore, Robo1 and miR-29a may serve as diagnostic or therapeutic targets for gastric cancer.

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Circulating miR-29a, Among Other Up-Regulated MicroRNAs, Is the Only Biomarker for Both Hypertrophy and Fibrosis in Patients With Hypertrophic Cardiomyopathy

Cited by 266 publications

References 40 publications

Impact of Demographic Features, Lifestyle, and Comorbidities on the Clinical Expression of Hypertrophic Cardiomyopathy

Impact of Demographic Features, Lifestyle, and Comorbidities on the Clinical Expression of Hypertrophic Cardiomyopathy

Altered microRNA and gene expression in the follicular fluid of women with polycystic ovary syndrome

MicroRNA-29a inhibits cell migration and invasion via targeting Roundabout homolog 1 in gastric cancer cells

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