Circulating miR-26a, miR-106b, miR-107 and miR-133b stratify hepatocellular carcinoma patients according to their response to transarterial chemoembolization

Ali, Hamdy; Emam, Ahmed A.; Zeeneldin, Ahmed A.; Srour, Reham; Tabashy, Reda; Desouky, Eman D. El; Elmageed, Zakaria Y. Abd; Abdel-Wahab, A.

doi:10.1016/j.clinbiochem.2019.01.002

Cited by 25 publications

(23 citation statements)

References 32 publications

(37 reference statements)

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“…In line with our data, it was recently shown that increased serum levels of miR-107 indicate response to TACE treatment [ 18 ]. In this study, several miRNAs were investigated for their potential to predict outcome to TACE treatment.…”

Section: Discussionsupporting

confidence: 92%

Serum levels of circulating microRNA-107 are elevated in patients with early-stage HCC

et al. 2021

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Background Early detection of hepatocellular carcinoma (HCC), the most common primary liver malignancy, is crucial to offer patients a potentially curative treatment strategy such as surgical resection or liver transplantation (LT). However, easily accessible biomarkers facilitating an early diagnosis of HCC as well as a reliable risk prediction are currently missing. The microRNA(miR)-107 has recently been described as a driver of HCC in both murine and human HCC but data on circulating miR-107 in HCC patients are scarce. In the present study, we evaluated a potential diagnostic and/or prognostic role of circulating miR-107 in patients undergoing tumor resection or LT for early-stage HCC. Methods The Kmplot bioinformatic tool was used to query publicly available databases (including TCGA, GEO and EGA) in order to analyse the prognostic value of tumoral miR-107 expression in HCC patients (n = 372). Serum levels of miR-107 were measured by qPCR in n = 45 HCC patients undergoing surgical tumor resection (n = 37) or LT (n = 8) as well as n = 18 healthy control samples. Results were correlated with clinical data. Results A high tumoral expression of miR-107 was associated with a significantly better overall survival compared to patients with low miR-107 expression levels (HR 0.69, 95% CI 0.48–0.99, p = 0.041). In addition, serum levels of miR-107 were significantly higher in HCC patients when compared to healthy controls. However, miR-107 serum levels in HCC patients were independent of different disease etiology, tumor stage or tumor grading. HCC patients with baseline miR-107 expression levels above a calculated ideal prognostic cut-off value (9.82) showed a clear trend towards an impaired overall survival (p = 0.119). Conclusion Tumoral miR-107 expression levels are a potential prognostic marker in early stage HCC. Furthermore, we describe a potential role of circulating miR-107 levels as a diagnostic biomarker in patients with early-stage HCC.

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Section: Discussionsupporting

confidence: 92%

Serum levels of circulating microRNA-107 are elevated in patients with early-stage HCC

et al. 2021

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“…A recent study showed that serum levels of miR-106b, miR-107, and miR-133b were elevated in patients with HCC who responded to TACE, with miR-26a being elevated in nonresponders. miR-26a and miR-133b exhibited the highest diagnostic performance, with miR-133b further distinguishing complete responders from partial responders and non-responders (AUC ≥ 0.90) [151]. Other studies have indicated that a combinatory analysis of plasma levels of miR-21, miR-26a, and miR-29a-3p, or even miR-122 alone, may predict early TACE refractoriness in patients with TACE-treated HCC [152].…”

Section: Cfdnamentioning

confidence: 96%

Liquid Biopsies in Hepatocellular Carcinoma: Are We Winning?

Mocan

Simão

Castro

et al. 2020

JCM

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Hepatocellular carcinoma (HCC) represents the sixth most common cancer worldwide and the third most common cause of cancer-related death. One of the major problems faced by researchers and clinicians in this area is the lack of reliable disease biomarkers, which would allow for an earlier diagnosis, follow-up or prediction of treatment response, among others. In this regard, the “HCC circulome”, defined as the pool of circulating molecules in the bloodstream derived from the primary tumor, represents an appealing target, the so called liquid biopsy. Such molecules encompass circulating tumor proteins, circulating tumor cells (CTCs), extracellular vesicles (EVs), tumor-educated platelets (TEPs), and circulating tumor nucleic acids, namely circulating tumor DNA (ctDNA) and circulating tumor RNA (ctRNA). In this article, we summarize recent findings highlighting the promising role of liquid biopsies as novel potential biomarkers in HCC, emphasizing on its clinical performance.

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“…There are almost no reports on the relationship between miR-107 and NSC differentiation, and to date, most studies on miR-107 have been related to cancer. A growing body of evidence indicates that aberrant miR-107 expression plays a key role in cancers, including breast cancer [ 18 ], gastric cancer [ 16 ], cervical cancer [ 36 ], hepatocellular carcinoma [ 2 ], and non-small cell lung cancer [ 35 ]. Prendecki et al indicated that altered miR-107 levels may be a marker of the neurodegenerative process during the course of AD, which is associated with amyloid β metabolism and excessive cell cycle progression [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Expression and function of Ndel1 during the differentiation of neural stem cells induced by hippocampal exosomesticle

Wang

et al. 2021

Stem Cell Res Ther

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Background In the brain of adult mammals, neural stem cells persist in the subventricular zone of the lateral ventricle and the subgranular zone of the dentate gyrus, which are specialized niches with proliferative capacity. Most neural stem cells are in a quiescent state, but in response to extrinsic stimuli, they can exit from quiescence and become reactivated to produce new neurons, so neural stem cells are considered to be a potential source for cell replacement therapy of many nervous system diseases. We characterized the expression of Ndel1 during the differentiation of neural stem cells induced by hippocampus exosomes, and assessed the effect of Ndel1 on neural stem cells differentiation. Methods Hippocampal exosomes were isolated and extracted, and co-cultured exosomes with neural stem cells. Western blot, flow cytometry, and immunofluorescence analyses were used to analyze expression of neuronal markers. Further, utilizing high-throughput RNA sequencing technology, we found that nudE neurodevelopment protein 1-like 1 was significantly upregulated in exosomes derived from denervated hippocampus, and then characterized its mechanism and function during neural stem cells differentiation by qRT-PCR, western blot, flow cytometry, and immunofluorescence analyses. Results Our results revealed that exosomes of denervated hippocampus promoted the differentiation of neural stem cells into neuron. Hence, we identified that nudE neurodevelopment protein 1-like 1 was significantly upregulated and highly expressed in the nervous system. In addition, we found that miR-107-3p may regulate neural stem cell differentiation by targeting Ndel1. Conclusions Our results revealed that deafferentation of the hippocampal exosomes co-cultured with neural stem cells could promote them to differentiate into neurons. Hence, we found that miR-107-3p may regulate neural stem cells differentiation by targeting Ndel1. Importantly, Ndel1 enhanced spatial learning and hippocampal neurogenesis in rats after fimbria fornix transection in vivo. These findings set the stage for a better understanding of neurogenesis, a process that 1 day may inspire new treatments for central nervous system diseases.

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Circulating miR-26a, miR-106b, miR-107 and miR-133b stratify hepatocellular carcinoma patients according to their response to transarterial chemoembolization

Cited by 25 publications

References 32 publications

Serum levels of circulating microRNA-107 are elevated in patients with early-stage HCC

Serum levels of circulating microRNA-107 are elevated in patients with early-stage HCC

Liquid Biopsies in Hepatocellular Carcinoma: Are We Winning?

Expression and function of Ndel1 during the differentiation of neural stem cells induced by hippocampal exosomesticle

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