Circulating miR-21, miR-146a and Fas ligand respond to postmenopausal estrogen-based hormone replacement therapy – A study with monozygotic twin pairs

Kangas, Reeta; Pöllänen, Eija; Rippo, Maria Rita; Lanzarini, Catia; Prattichizzo, Francesco; Niskala, Paula; Jylhävä, Juulia; Sipilä, Sarianna; Kaprio, Jaakko; Procopio, Antonio Domenico; Capri, Miriam; Franceschi, Claudio; Olivieri, Fabiola; Kovanen, Vuokko

doi:10.1016/j.mad.2014.11.001

Cited by 48 publications

(43 citation statements)

References 48 publications

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“…In this relatively young research division, only moderate progress and information were achieved. Nevertheless, there is increasing evidence that both steroids are implicated in the regulation of circulating and local miRNAs with respect to breast cancer [100,101], hepatocellular carcinoma [102], placental and ovarian dysfunction [87,103,104] and hormone replacement therapy [105]. Vice versa, miRNAs contribute to the regulation of steroid signaling by affecting post-transcriptional regulation of progesterone and estrogen receptors [106,107].…”

Section: Gonadal Steroids and Inflammasome Regulationmentioning

confidence: 99%

Inflammasomes are neuroprotective targets for sex steroids

Slowik

Beyer

2015

The Journal of Steroid Biochemistry and Molecular Biology

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Section: Gonadal Steroids and Inflammasome Regulationmentioning

confidence: 99%

Inflammasomes are neuroprotective targets for sex steroids

Slowik

Beyer

2015

The Journal of Steroid Biochemistry and Molecular Biology

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“…Another set of studies has shown changes in miR profiles in different conditions [10] . As we showed in our recent twin paper, the levels of specific circulating miRs are partly genetically regulated (miR-21: r=0.66, miR-146a: r=0.38) [2] . Due to that, setting common reference values for normal and abnormal miR levels for the clinical use, might be challenging.…”

Section: Research Highlightmentioning

confidence: 64%

“…It has also been stated that miRs deliver their messages similarly to hormones themselves. In our recent study we demonstrated that circulating miR-21 (onco-miR) and miR-146a (inflamma-miR) levels differ between postmenopausal monozygotic twin sisters whose hormonal status differs from each other, especially what comes to systemic estrogen concentrations [2] . We have also shown with the same data, that HRT enhances the IGF-1 signaling by lowering the levels of miR-223 and miR-182 in the skeletal muscle [3] .…”

mentioning

confidence: 99%

Aging and systemic hormonal status affects the circulating miR-21, miR-146a and FasL levels

Kangas

Pöllänen²,

Kovanen³

2015

RNA Dis

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MicroRNAs are small molecules, found in all cell types and body fluids, which most commonly affect negatively to gene expressions by translational repression. Their role in various physiological conditions and diseases has been emphasized during the last twenty years. In our recent studies with postmenopausal monozygotic twin sisters (n=11), we have investigated how different systemic hormonal status affects the levels of specific circulating microRNAs and other molecules related to inflammation and apoptosis, both processes associated with aging. Our results have shown that the systemic levels of miR-21, miR-146a and Fas ligand are lower within the postmenopausal women who are using estrogen-based hormonal replacement (HRT), compared to their non-using co-twins (p=0.018, p=0.039, p=0.021, respectively). To get further knowledge about the aging effect, we also measured the same markers from the premenopausal control women (n=8), with natural hormonal status, and found out that the inflammatory profile was healthier among the young women and that the serum miR-21 profile was more similar with the HRT users than non-users, and miR-146 and FasL profile more similar to non-users. These findings demonstrate that HRT has effects on the circulating inflammation related regulatory molecules. Whether we can state that the effects are clearly positive or negative, needs further investigations and understanding of the regulatory system. To cite this article: R.Kangas, et al. Aging and systemic hormonal status affects the circulating miR-21, miR-146a and FasL levels. RNA Dis 2015; 2: e552.

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“…The RNA extraction methods and miR-21 and miR-146a analyses have been described previously [26]. Briefly, total RNA was isolated from 100 µ l of serum with a total RNA purification kit (Norgen Biotek Corporation, Thorold, ON, Canada) according to the manufacturer's protocol.…”

Section: Methodsmentioning

confidence: 99%

Declining Physical Performance Associates with Serum FasL, miR-21, and miR-146a in Aging Sprinters

Kangas

Törmäkangas

Heinonen

et al. 2017

BioMed Research International

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Aging is associated with systemic inflammation and cellular apoptosis accelerating physiological dysfunctions. Whether physically active way of life affects these associations is unclear. This study measured the levels of serum inflammatory and apoptotic molecules, their change over 10 years, and their associations with physical performance in sprint-trained male athletes. HsCRP, cell counts, HGB, FasL, miR-21, and miR-146a were measured cross-sectionally (n = 67, 18–90 yrs) and serum FasL, miR-21, and miR-146a and their aging-related associations with physical performance were assessed over a 10-year follow-up (n = 49, 50–90 yrs). The cross-sectional study showed positive age correlations for neutrophils and negative for lymphocytes, red blood cells, HGB, FasL, and miR-146a. During the 10-year follow-up, FasL decreased (P = 0.017) and miR-21 (P < 0.001) and miR-146a (P = 0.005) levels increased. When combining the molecule levels, aging, and physical performance, FasL associated with countermovement jump and bench press (P < 0.001), miR-21 and miR-146a with knee flexion (P = 0.023; P < 0.001), and bench press (P = 0.004; P < 0.001) and miR-146a with sprint performance (P < 0.001). The studied serum molecules changed in an age-dependent manner and were associated with declining physical performance. They have potential as biomarkers of aging-related processes influencing the development of physiological dysfunctions. Further research is needed focusing on the origins and targets of circulating microRNAs to clarify their function in various tissues with aging.

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Circulating miR-21, miR-146a and Fas ligand respond to postmenopausal estrogen-based hormone replacement therapy – A study with monozygotic twin pairs

Cited by 48 publications

References 48 publications

Inflammasomes are neuroprotective targets for sex steroids

Inflammasomes are neuroprotective targets for sex steroids

Aging and systemic hormonal status affects the circulating miR-21, miR-146a and FasL levels

Declining Physical Performance Associates with Serum FasL, miR-21, and miR-146a in Aging Sprinters

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