Circulating miR-21 and eNOS in subclinical atherosclerosis in patients with hypertension

Cengiz, Mahir; Yavuzer, Serap; Avcı, Burçak Kılıçkıran; Yürüyen, Mehmet; Yavuzer, Hakan; Dikici, Süleyman; Karataş, Ömer Faruk; Özen, Mustafa; Uzun, Hafize; Öngen, Zeki

doi:10.3109/10641963.2015.1036064

Cited by 75 publications

(46 citation statements)

References 36 publications

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“…In subclinical atherosclerosis miR-21 was found increased (26). In our study, patients enrolled in ATHE group display lower levels of miR-21 vs control.…”

Section: Discussion and Conclusion Discussion And Conclusionsupporting

confidence: 49%

Circulating miR-21 and TGF-b1 expression levels in patients with cardiovascular diseases

Huică¹,

Iancu²,

Botezatu³

et al. 2015

JTMR

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Recent works focus on the role microRNAs (miRNAs) play in regulation of cardiovascular pathology. One of the miRNAs highly expressed in cardiovascular system is miR-21. The aim of this study was to investigate the relationship between the expression level of miR-21 in cardiac disease and correlate with TGF-β1, one of its targets. Methods: Plasma samples from patients with CVD (cardiovascular diseases) established according to clinical and paraclinical exams were subjected to total RNAs isolation. miR-21 expression levels in patients with CVD and in healthy control subjects were quantified on ABI 7300 real-time PCR (Applied BioSystems) and normalized to RNU-43. TGF-β1 was predicted as potential target of miR-21 using MicroCosm TARGETS and quantified in TaqMan system (normalized to GAPDH). Statistical analysis was performed with GraphPad Prism. Results: Significant lower levels of miR-21 were found in CVD patients vs. controls (p<0.0001). An inverse correlation between miR-21 and TGF-β1 expression levels was noted in CVD patients. miR-21 levels vs. control were significantly different for ATHE, CAD and ATH groups (p=0.0016, 0.0058 and 0.0032 respectively) and correlated with TGF-β1 expression and cardiac overload. Conclusions:The results of this study suggest a good correlation between miR-21 and TGF-β1 in cardiovascular diseases.

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“…In subclinical atherosclerosis miR-21 was found increased (26). In our study, patients enrolled in ATHE group display lower levels of miR-21 vs control.…”

Section: Discussion and Conclusion Discussion And Conclusionsupporting

confidence: 49%

Circulating miR-21 and TGF-b1 expression levels in patients with cardiovascular diseases

Huică¹,

Iancu²,

Botezatu³

et al. 2015

JTMR

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“…To avoid the exclusion of some relevant miRNAs involved in PM 10 exposure and BP regulation, 28 candidate miRNAs were chosen for validation, on the basis of their significant FDR P -values and their roles in BP regulation, CVD, and air pollution exposure (Pan et al, 2012, Deng et al, 2014, Neppl and Wang, 2014, Cakmak et al, 2015, Cengiz et al, 2015) (see Supplemental file: Supplemental Table 3). …”

Section: Resultsmentioning

confidence: 99%

MicroRNAs are associated with blood-pressure effects of exposure to particulate matter: Results from a mediated moderation analysis

Motta

Favero

Dioni

et al. 2016

Environmental Research

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AimsExposure to particulate air pollution is associated with increased blood pressure (BP), a well-established risk factor for cardiovascular disease. To elucidate the mechanisms underlying this relationship, we investigated whether the effects of particulate matter of less than 10 μm in aerodynamic diameter (PM10) on BP are mediated by microRNAs.Methods and resultsWe recruited 90 obese individuals and we assessed their PM10 exposure 24 and 48 h before the recruitment day. We performed multivariate linear regression models to investigate the effects of PM10 on BP. Using the TaqMan® Low-Density Array, we experimentally evaluated and technically validated the expression levels of 377 human miRNAs in peripheral blood. We developed a mediated moderation analysis to estimate the proportion of PM10 effects on BP that was mediated by miRNA expression.PM10 exposure 24 and 48 h before the recruitment day was associated with increased systolic BP (β=1.22 mmHg, P=0.019; β=1.24 mmHg, P=0.019, respectively) and diastolic BP (β=0.67 mmHg, P=0.044; β=0.91 mmHg, P=0.007, respectively). We identified nine miRNAs associated with PM10 levels 48 h after exposure. A conditional indirect effect (CIE=−0.1431) of PM10 on diastolic BP, which was mediated by microRNA-101, was found in individuals with lower values of mean body mass index.ConclusionsOur data provide evidence that miRNAs are a molecular mechanism underlying the BP-related effects of air pollution exposure, and indicate miR-101 as epigenetic mechanism to be further investigated.

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“…Wang, Wang, & Yang, 2013). miRNA-mediated gene regulation has been implicated in inflammation, cholesterol homeostasis, oxidative stress, and hypertension; all key biological processes underlying CVD (Cengiz, et al, 2015; Heggermont & Heymans, 2012; Lai, et al, 2013; G. Liu & Abraham, 2013; Sen, et al, 2011; Vickers, et al, 2014; Y.…”

Section: Introductionmentioning

confidence: 99%

HDL and microRNA therapeutics in cardiovascular disease

Michell

Vickers

2016

Pharmacology & Therapeutics

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microRNAs (miRNA) are small non-coding RNAs (sRNA) that post-transcriptionally regulate gene (mRNA) expression and are implicated in many biological processes and diseases. Many miRNAs have been reported to be altered in cardiovascular disease (CVD); both cellular and extracellular miRNA levels are affected by hypercholesterolemia and atherosclerosis. We and other groups have reported that lipoproteins transport miRNAs in circulation and these lipoprotein signatures are significantly altered in hypercholesterolemia and coronary artery disease (CAD). Extracellular miRNAs are a new class of potential biomarkers for CVD; however, they may also be new drug targets as high-density lipoproteins (HDL) transfer functional miRNAs to recipient cells in an endocrine-like form of intercellular communication that likely suppresses vascular inflammation. Recently, RNA-based drugs have emerged as the next frontier in drug therapy, and there are many miRNA inhibitors and mimics in clinical development. Here, we discuss specific miRNA drug targets and how their manipulation may impact CVD. We also address the potential for manipulating HDL-miRNA levels to treat CVD and the use of HDL as a delivery vehicle for RNA and chemical drugs. Finally, we outline the current and future challenges for HDL and miRNA-based therapeutics for the prevention and treatment of CVD.

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Circulating miR-21 and eNOS in subclinical atherosclerosis in patients with hypertension

Cited by 75 publications

References 36 publications

Circulating miR-21 and TGF-b1 expression levels in patients with cardiovascular diseases

Circulating miR-21 and TGF-b1 expression levels in patients with cardiovascular diseases

MicroRNAs are associated with blood-pressure effects of exposure to particulate matter: Results from a mediated moderation analysis

HDL and microRNA therapeutics in cardiovascular disease

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