Circulating miR-135 May Serve as a Novel Co-biomarker of HbA1c in Type 2 Diabetes

Monfared, Yousef Khazaei; Honardoost, Maryam; Sarookhani, Mohammad Reza; Farzam, Seyed Amir

doi:10.1007/s12010-019-03163-2

Cited by 11 publications

(9 citation statements)

References 23 publications

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“…Además, el nivel de miR-135 mostró un valor predictivo negativo significativo para los grupos con diabetes y prediabetes, lo que proporciona una gran confianza en los resultados negativos obtenidos. (13)…”

Section: Resultsunclassified

MicroRNA for the early detection of type 2 diabetes mellitus: literature review

Martínez Altamirano,

Romo López

2023

Salud, Ciencia y Tecnología

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Introducción: La Diabetes Mellitus (DM) es una alteración del metabolismo de la glucosa de múltiple etiología, representa más del 90% de los casos de diabetes alrededor del mundo. Estudios actuales han propuesto a los microARN (miARNs), como posibles biomarcadores tempranos de la enfermedad.Objetivo: analizar la literatura sobre el uso de miARNs como biomarcadores en la detección temprana de la Diabetes Mellitus tipo 2 (DMT2). Métodos: Se llevaron a cabo búsquedas exhaustivas en bases de datos científicas y se seleccionaron nueve estudios que cumplieron con los criterios de inclusión. Estos estudios examinaron los perfiles de expresión de miARNs específicos y su utilidad como marcadores diagnósticos y pronósticos. Resultados: Revelaron que ciertos miARNs, como miR-21, miR-122, miR-148b-3p, miR-27a-3p y miR-135, mostraron cambios significativos en su expresión en pacientes con DMT2 y prediabetes en comparación con individuos sanos. Estos miARNs también se asociaron con procesos metabólicos clave, como la regulación de la señalización de insulina, la resistencia a la insulina y la inflamación. Conclusiones: Estos hallazgos respaldan el potencial de los miARNs como biomarcadores para la detección temprana, pronóstico, manejo de la DMT2 y la prediabetes. Sin embargo, se requieren más investigaciones para validar y comprender mejor la relevancia clínica de los miARNs en estas enfermedades metabólicas.

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Section: Resultsunclassified

MicroRNA for the early detection of type 2 diabetes mellitus: literature review

Martínez Altamirano,

Romo López

2023

Salud, Ciencia y Tecnología

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“…Sarookhani and colleagues reported increased expression of miR-135a in the plasma of patients with T2DM and prediabetic patients compared to healthy controls [138]. Monfared et al reported similar results and also found a correlation between serum miR-135a levels and HbA1c levels in prediabetic patients [139]. In another study, Monfared and colleagues reported that miR-135a in saliva could be used as a biomarker for T2DM [140].…”

Section: Mir-135mentioning

confidence: 86%

“…Under atherosclerotic conditions, it downregulates eNOS, inhibits vascular repair mechanisms and promotes VSMC calcification [139]. Minami and colleagues reported elevated levels of miR-221 in the serum of patients with atherosclerosis [144].…”

Section: Mir-221mentioning

confidence: 99%

Review of microRNAs and lncRNAs in atherosclerosis, diabetes, and other inflammatory conditions

Letonja,

Petrovič

2023

Preprint

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It is generally accepted that atherosclerosis is a chronic inflammatory disease. The link between atherosclerosis and other inflammatory diseases such as psoriasis, type 2 diabetes mellitus (T2DM), and rheumatoid arthritis (RA) via metabolic, inflammatory, and immunoregulatory pathways is well established. The aim of our review was to summarize the associations between selected microRNAs (miRs) and long non-coding RNAs (lncRNAs) and atherosclerosis, psoriasis, T2DM, and RA. MicroRNAs are short noncoding RNA molecules involved in cell signaling, intracellular communication, and gene expression. We reviewed the role of miR-146a, miR-210, miR-143, miR-223, miR-126, miR-21, miR-155, miR-145, miR-200, miR-133, miR-135, miR-221, miR-424, and let-7 in atherosclerosis, psoriasis, T2DM, and RA. LncRNAs are RNA transcripts longer than 200 nucleotides that are involved in cellular processes such as apoptosis, metabolism, inflammation, cell differentiation, and proliferation. We evaluated the role of lncRNA-H19, lncRNA-MEG3, lncRNA-UCA1, and lncRNA-XIST in atherosclerosis and psoriasis, T2DM, and RA. Extracellular vesicles (EVs) are a method of intracellular signal transduction. Their function depends on surface expression, cargo, and the cell from which they originate.

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“…In the pathological process of diabetes, miR-135 is involved in the process of insulin resistance [30]. In a case study, miR-135a was found to be signi cantly elevated in the plasma of diabetic patients compared to healthy controls [31], not only as a marker of glycemic control, but also to target SIRT1 and affect cell proliferation and apoptosis [32]. However, only a few studies have explored why exosomal miRNAs can regulate neovascularization.…”

Section: Discussionmentioning

confidence: 99%

Exosomal miR-135a-5p promotes neovascularization via targeting SIRT1 in diabetic retinopathy

Tong

et al. 2022

Preprint

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Background: Diabetic retinopathy (DR) is one of the most serious microvascular complications of diabetes mellitus. Retinal neovascularization （RNV) under the continuous influence of hyperglycemia is the main cause of blindness in DR patients, but the mechanism of neovascularization has not been fully elucidated yet. In this study, we investigated the effect of exosomes secreted by human retinal pigmented epithelial (ARPE-19) cells at high glucose concentrations on in vitro angiogenesis of human umbilical vein endothelial cells(HUVECs). The role of exosomal miRNAs in the pathogenesis of DR was explored.Methods: Exosomes were extracted from the supernatant of ARPE-19 cells by differential centrifugation. Confocal microscopy was used to observe whether exosomes could be taken up by HUVEC, and the effects of exosomes on HUVEC angiogenesis under three sets of glucose concentrations were observed by co-culture and in vitro angiogenesis. The expression of 21 candidate miRNAsin exosomes and 22 candidate mRNAsin HUVEC after the effect of three sets of glucose concentrations was examined by qRT-PCR. Prediction of miRNA-targeted regulatory mRNAs by bioinformatics. Regulation of miR-135a-5p expression by transfection of small interfering RNA and miRNA mimic. Western blot and qRT-PCR were performed to detect the expression of SITR1.Results: Confocal images show that high glucose-induced exosomes （HG-exo） can be taken up by HUVECs and promote the tube formation of HUVECs in vitro. The results of qRT-PCR showed that the expressions of miR-9-3p, miR-135a-5p, miR-134-5p, miR-195-3p, miR-181c-5p and miR-132-3p were upregulated in HG-exo (P < 0.05). The expressions of HIF-1α, SIRT1 and SPRED1 in HUVECs co-cultured with HG-exo were decreased (P < 0.05). Bioinformatics analysis indicated that miR-135a-5p binds to the SIRT1 3'UTR region. We verified this by transfection experiments and showed that miR-135a-5p could inhibit the expression of SIRT1 mRNA and protein.Conclusions:In the presence of high glucose, exosomal miR135a-5p secreted by ARPE-19 cells targeted the SIRT1 3'UTR region and inhibited SIRT1 expression, which in turn promoted neovascularization.

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Circulating miR-135 May Serve as a Novel Co-biomarker of HbA1c in Type 2 Diabetes

Cited by 11 publications

References 23 publications

MicroRNA for the early detection of type 2 diabetes mellitus: literature review

MicroRNA for the early detection of type 2 diabetes mellitus: literature review

Review of microRNAs and lncRNAs in atherosclerosis, diabetes, and other inflammatory conditions

Exosomal miR-135a-5p promotes neovascularization via targeting SIRT1 in diabetic retinopathy

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