Circulating microRNAs (miR-21, miR-223, miR-885-5p) along the clinical spectrum of HCV-related chronic liver disease in Egyptian patients

Nasser, Mona; Mohamed, Ahmed Mahmoud; Attia, Dina; Esmat, Gamal; Khairy, Ahmed

doi:10.1016/j.ajg.2019.11.003

Cited by 15 publications

(16 citation statements)

References 39 publications

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“…MiRNAs show variable dysregulation patterns with HCV-related liver illness and might be contribute as potential noninvasive markers for progression of liver fibrosis. 25 In the current study, miR-199 showed a significant upregulation in late stages of fibrosis than in early stages, while both miRNAs-181c and 448 were downregulated in late stages of fibrosis. ROC curve analysis showed high sensitivity and specificity (89.47, 99.11), indicating significance of miR-199 as a potential marker in diagnosis of fibrosis.…”

Section: Discussionsupporting

confidence: 43%

“…Therefore, recognition of non‐invasive potential markers with good accuracy for assessment of liver fibrosis is a very important goal. MiRNAs show variable dysregulation patterns with HCV‐related liver illness and might be contribute as potential non‐invasive markers for progression of liver fibrosis 25 . In the current study, miR‐199 showed a significant upregulation in late stages of fibrosis than in early stages, while both miRNAs‐181c and 448 were downregulated in late stages of fibrosis.…”

Section: Discussionmentioning

confidence: 44%

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Serum MicroRNAs as predictors for fibrosis progression and response to direct‐acting antivirals treatment in hepatitis C virus genotype‐4 Egyptian patients

et al. 2020

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Background MicroRNA (miRNAs) are small non‐coding molecules that play an important role in hepatitis C virus (HCV) replication and liver diseases progression. The current study aimed to evaluate serum miRNAs as potential biomarkers for diagnosis, monitoring of fibrosis progression and prediction of responses to direct‐acting antivirals (sofosbuvir + daclatasvir + ribavirin) in HCV genotype‐4 patients. Methods The serum levels of four miRNAs (miRNA‐21, 199, 448 and 181c) were assessed in 150 HCV patients and 50 healthy controls using quantitative real‐time PCR. The diagnostic accuracy was determined using receiver operating characteristic (ROC) curve. Results The four studied miRNAs showed significant upregulation in HCV patients compared with controls. There were significant upregulation of MiR‐199 and significant downregulation of miR‐448 in late stages of fibrosis with high diagnostic accuracy (area under the curve “AUC” = 0.989%; P < .001) and (AUC = 0.0.672; P > .001), respectively. Regarding response to treatment, only miR‐199 showed a significant upregulation in non‐responder patients with high diagnostic accuracy (AUC = 0.968; P < .001). Conclusion miR‐199 and miR‐448 could serve as valuable non‐invasive biomarkers for assessment of liver fibrosis progression. Additionally, miR‐199 could be also a potential biomarker for assessment of treatment efficacy among HCV patients. Therefore, miR‐199 and miR‐448 serum levels should be considered during the treatment of HCV genotype‐4 patients in Egypt and the world.

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Section: Discussionsupporting

confidence: 43%

Section: Discussionmentioning

confidence: 44%

Serum MicroRNAs as predictors for fibrosis progression and response to direct‐acting antivirals treatment in hepatitis C virus genotype‐4 Egyptian patients

et al. 2020

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“…Upregulation in HCV infection was previously described by El-Diwany and colleagues [26]. It was also among four microRNAs (miR-122, miR-885-5p, miR-221 and miR-22) in a panel demonstrating high diagnostic accuracy for the early detection of HCC in liver cirrhosis HCV patients [27] and one of three circulating microRNA markers of HCV-related chronic liver disease in Egyptian patients, suggesting that miR-885-5p could be a potential marker for advanced liver damage [28].…”

Section: Comparison Of Blood Donor and Patient Sets Of Samplesmentioning

confidence: 75%

Expression Profiles of Exosomal MicroRNAs from HEV- and HCV-Infected Blood Donors and Patients: A Pilot Study

McGowan

Simpson

Petrík

2020

Viruses

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Exosomes seem to play an important role in hepatits C virus (HCV) and hepatitis E virus (HEV) infection by shielding their cargo from the host immune responses, with microRNAs being key exosomal components. Little is known about their involvement in a mixed HCV/HEV infection or at the early stages of infection, such as in asymptomatic blood donors (BDs). To obtain preliminary data, we have compared the exosomal microRNA expression profiles in four each of HCV RNA-positive, HEV RNA-positive and negative blood donors and four patients, one of whom was a rare patient with HCV/HEV co-infection. Exosomes were purified from sera by a combination of a precipitation and density gradient centrifugation and exosomal microRNA was analysed using Taqman array cards. Out of 33 deregulated miRNAs, miR-885-5p and miR-365 were upregulated in HCV BDs, miR-627-5p was downregulated in HCV BD and miR-221 was downregulated in HCV patients and BDs. In HEV infection, miR-526b appeared specifically downregulated. Six miRNAs (miR-628-3p, miR-194, miR-151-3p, miR-512-3p, miR-335 and miR-590) indicated a potential involvement in both infections. First time preliminary data on pre- and post-antiviral treatment exosomal microRNA profiles of the HEV/HCV co-infected patient revealed a pool of 77 upregulated and 43 downregulated miRNAs to be further investigated for their potential roles in these viral infections.

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“…miR-885-5p has been demonstrated to be aberrantly expressed in several diseases, including cancers [27][28][29]. For instance, miR-885-5p functioned as a tumor suppressor in hepatocellular carcinoma by suppressing cell metastasis in vitro and in vivo [30].…”

Section: Discussion/conclusionmentioning

confidence: 99%

Identification of miR-885-5p as a Tumor Biomarker: Regulation of Cellular Function in Cervical Cancer

Wang

2021

Gynecol Obstet Invest

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Objectives: MicroRNAs were revealed as biomarkers for early detection or prognosis predictors of cancer and were involved in the progression of cancer. The present study investigated the expression pattern, potential clinical, and functional role of miR-885-5p in cervical cancer. Design: A total of 115 pairs of cervical cancer tissue specimens and adjacent non-tumor paracancerous tissue specimens were collected from the cervical cancer patients who underwent surgical resection or biopsy without preoperative systemic therapy at Maternity and Child Health Care of Zaozhuang from 2012 to 2014. Participants/Materials, Setting, Methods: The expression levels of miR-885-5p in cervical cancer were measured using the qRT-PCR assay. A follow-up study was conducted and the Kaplan-Meier method with log-rank test was used to analyze the potential clinical significance of miR-885-5p in cervical cancer. The functional experiments including CCK-8, Transwell migration, and invasion assays were used to investigate the biological function of miR-885-5p in cervical cancer cells. Results: miR-885-5p expression was decreased in tumor tissues and tumor cell lines compared to normal control. Low expression of miR-885-5p was related to lymph node metastasis, late FIGO stage, and shorter overall survival outcome. Ascending expression of miR-885-5p inhibited the proliferative, migratory, and invasive abilities of cervical cancer cells, while downregulation of miR-885-5p promoted these cellular abilities of cervical cancer cells in vitro. Limitations: The patient population size was limited, thus the clinical significance of miR-885-5p requires further verification. Secondly, the precise mechanism of miR-885-5p in cervical cancer still exclusive. In future studies, a larger sample size will be required to confirm the prognostic value of miR-885-5p in cervical cancer, and the possible targets, as well as the detailed mechanism of miR-885-5p, will be investigated. Conclusions: miR-885-5p expression was decreased in cervical cancer and downregulation of miR-885-5p promoted the progression of cervical cancer cells. miR-885-5p may be an independent prognostic predictor and therapeutic target for treating cervical cancer.

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Circulating microRNAs (miR-21, miR-223, miR-885-5p) along the clinical spectrum of HCV-related chronic liver disease in Egyptian patients

Cited by 15 publications

References 39 publications

Serum MicroRNAs as predictors for fibrosis progression and response to direct‐acting antivirals treatment in hepatitis C virus genotype‐4 Egyptian patients

Serum MicroRNAs as predictors for fibrosis progression and response to direct‐acting antivirals treatment in hepatitis C virus genotype‐4 Egyptian patients

Expression Profiles of Exosomal MicroRNAs from HEV- and HCV-Infected Blood Donors and Patients: A Pilot Study

Identification of miR-885-5p as a Tumor Biomarker: Regulation of Cellular Function in Cervical Cancer

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