Circulating microRNAs in lung cancer: Prospects for diagnosis, prognosis, and prediction of antitumor treatment efficacy

Vv, Vlasov; EIu, Rykova; Aa, Ponomareva; Zaporozhchenko, Ivan A.; Es, Morozkin; Чердынцева, Н. В.; Pp, Laktionov

doi:10.1134/s0026893315010161

Cited by 10 publications

(5 citation statements)

References 36 publications

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“…In the subgroup of miRNA detection methods, only one study used microarrays, while the rest used qRT-PCR, Here, the number of studies needs to be expanded for analysis. Moreover, qRT-PCR is more sensitive than microarrays and is a widely used method for the detection of microRNAs, In addition, it is considered the gold standard technology to verify the results of microarray analysis [ 57 , 58 ]. Although most previous studies have chosen U6 as the reference gene for normalization, our subgroup analysis showed that other reference genes (Sen: 88%, Spe: 86%, DOR: 44, AUC: 0.93) showed better diagnostic ability than U6(Sen: 87%, Spe: 79%, DOR: 25, AUC: 0.86).…”

Section: Discussionmentioning

confidence: 99%

Circulating microRNAs as diagnostic biomarkers for melanoma: a systematic review and meta-analysis

Sun

et al. 2023

BMC Cancer

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Background Recent studies have shown that circulating microRNAs (miRNAs) can be used as diagnostic biomarkers for melanoma. This study aimed to evaluate the diagnostic value of circulating miRNAs for melanoma. Methods A comprehensive literature search was conducted and the quality of the included literature was evaluated using QUADAS-2 (Quality Assessment for diagnostic accuracy studies), and the diagnostic accuracy was assessed by pooled sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR), and area under the curve (AUC). We used Deeks’ funnel plot to evaluate publication bias. Results The meta-analysis included 10 articles covering 16 studies, and the results showed that circulating miRNAs provide high diagnostic accuracy for melanoma. The overall pooled sensitivity was 0.87 (95% CI: 0.82–0.91), specificity was 0.81 (95% CI: 0.77–0.85), PLR was 4.6 (95% CI: 3.7–5.8), NLR was 0.16 (95% CI: 0.11–0.23), DOR was 29 (95% CI: 18–49), and AUC was 0.90 (95% CI: 0.87–0.92), respectively. Subgroup analysis showed better diagnostic value in miRNA clusters, European population, plasma miRNAs, and upregulated miRNAs compared to other subgroups. Conclusions The results indicated that circulating microRNAs can be used as a non-invasive biomarker for the diagnosis of melanoma.

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Section: Discussionmentioning

confidence: 99%

Circulating microRNAs as diagnostic biomarkers for melanoma: a systematic review and meta-analysis

Sun

et al. 2023

BMC Cancer

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“…Several in vitro studies also reported the potential of MWCNTs to induce inhibition of proliferation, cell cycle delay, apoptosis, malignant transformation, genotoxicity and abnormal chromosome number by interfering with mitosis (cell division) in various in vitro and in vivo studies [ 59 , 101 , 102 , 104 – 111 ]. In fact, some of the miRNAs (e.g., ↑hsa-miR-20a-5p, ↑hsa-miR-21-5p, ↑hsa-miR-23a-5p, ↓hsa-miR-16-5p) and their mRNA targets aberrantly expressed in exposed workers are known to play critical roles in cell proliferation, apoptosis, tumor invasiveness and tumorigenesis as well as the pathogenesis of cancer including and NSCLC [ 103 , 112 – 120 ]. Taken together, the results presented in this paper may suggest the onset of carcinogenic risk in humans potentially exposed to MWCNTs.…”

Section: Discussionmentioning

confidence: 99%

Integrated Analysis of Dysregulated ncRNA and mRNA Expression Profiles in Humans Exposed to Carbon Nanotubes

et al. 2016

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BackgroundAs the application of carbon nanotubes (CNT) in consumer products continues to rise, studies have expanded to determine the associated risks of exposure on human and environmental health. In particular, several lines of evidence indicate that exposure to multi-walled carbon nanotubes (MWCNT) could pose a carcinogenic risk similar to asbestos fibers. However, to date the potential markers of MWCNT exposure are not yet explored in humans.MethodsIn the present study, global mRNA and ncRNA expression profiles in the blood of exposed workers, having direct contact with MWCNT aerosol for at least 6 months (n = 8), were compared with expression profiles of non-exposed (n = 7) workers (e.g., professional and/or technical staff) from the same manufacturing facility.ResultsSignificant changes in the ncRNA and mRNA expression profiles were observed between exposed and non-exposed worker groups. An integrative analysis of ncRNA-mRNA correlations was performed to identify target genes, functional relationships, and regulatory networks in MWCNT-exposed workers. The coordinated changes in ncRNA and mRNA expression profiles revealed a set of miRNAs and their target genes with roles in cell cycle regulation/progression/control, apoptosis and proliferation. Further, the identified pathways and signaling networks also revealed MWCNT potential to trigger pulmonary and cardiovascular effects as well as carcinogenic outcomes in humans, similar to those previously described in rodents exposed to MWCNTs.ConclusionThis study is the first to investigate aberrant changes in mRNA and ncRNA expression profiles in the blood of humans exposed to MWCNT. The significant changes in several miRNAs and mRNAs expression as well as their regulatory networks are important for getting molecular insights into the MWCNT-induced toxicity and pathogenesis in humans. Further large-scale prospective studies are necessary to validate the potential applicability of such changes in mRNAs and miRNAs as prognostic markers of MWCNT exposures in humans.

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“…Many researchers point to the important role of the miRNA axis in the progression of various cancers [93][94][95][96]. It is now clear that miRNAs could be evaluated as predictors of the response to therapy and as biomarkers.…”

Section: Discussionmentioning

confidence: 99%

Towards Unravelling the Role of ERα-Targeting miRNAs in the Exosome-Mediated Transferring of the Hormone Resistance

et al. 2021

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Hormone therapy is one of the most effective breast cancer treatments, however, its application is limited by the progression of hormonal resistance, both primary or acquired. The development of hormonal resistance is caused either by an irreversible block of hormonal signalling (suppression of the activity or synthesis of hormone receptors), or by activation of oestrogen-independent signalling pathways. Recently the effect of exosome-mediated intercellular transfer of hormonal resistance was revealed, however, the molecular mechanism of this effect is still unknown. Here, the role of exosomal miRNAs (microRNAs) in the transferring of hormonal resistance in breast cancer cells has been studied. The methods used in the work include extraction, purification and RNAseq of miRNAs, transfection of miRNA mimetics, immunoblotting, reporter analysis and the MTT test. Using MCF7 breast cancer cells and MCF7/T tamoxifen-resistant sub-line, we have found that some miRNAs, suppressors of oestrogen receptor signalling, are overexpressed in the exosomes of the resistant breast cancer cells. The multiple (but not single) transfection of one of the identified miRNA, miR-181a-2, into oestrogen-dependent MCF7 cells induced the irreversible tamoxifen resistance associated with the continuous block of the oestrogen receptor signalling and the activation of PI3K/Akt pathway. We suppose that the miRNAs-ERα suppressors may act as trigger agents inducing the block of oestrogen receptor signalling and breast cancer cell transition to an aggressive oestrogen-independent state.

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Circulating microRNAs in lung cancer: Prospects for diagnosis, prognosis, and prediction of antitumor treatment efficacy

Cited by 10 publications

References 36 publications

Circulating microRNAs as diagnostic biomarkers for melanoma: a systematic review and meta-analysis

Circulating microRNAs as diagnostic biomarkers for melanoma: a systematic review and meta-analysis

Integrated Analysis of Dysregulated ncRNA and mRNA Expression Profiles in Humans Exposed to Carbon Nanotubes

Towards Unravelling the Role of ERα-Targeting miRNAs in the Exosome-Mediated Transferring of the Hormone Resistance

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