Circulating microRNAs in acute appendicitis

Seyhan, Avni Uygar; Şener, Elif Funda; Bol, Oğuzhan; Taheri, Serpil; Topaloglu, Tugba; Tufan, Esra; Tahtasakal, Reyhan; Günay, Nahide Ekici; Karabulut, Hatice; Günay, Nurullah

doi:10.5505/tjtes.2017.22379

Cited by 3 publications

(2 citation statements)

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“…MicroRNAs are small RNA molecules involved in the regulation of gene expression. Some studies have identified specific microRNAs, such as miR-29c-3p that was reported to increase in the acute period of AA [68], as potential biomarkers for appendicitis. These microRNAs can be detected in blood or other bodily fluids, and their altered expression patterns have shown promise in distinguishing appendicitis from other conditions.…”

Section: Emerging Diagnostic Approaches For Acutementioning

confidence: 99%

Unveiling the Role of Inflammatory Mediators and Gut Microbiome in Appendicitis: Types and Applications in Clinical Scoring

Elwan,

Abdennour,

Akl

et al. 2023

Advanced Gut & Microbiome Research

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Appendicitis is a common medical condition that affects millions of people worldwide. It is characterized by inflammation of the appendix. The exact mechanisms that trigger the inflammatory response in appendicitis are not well understood, but it is known that inflammatory mediators play a crucial role in the development and progression of the disease. In this review, we provide a comprehensive overview of the current understanding of the role of inflammatory mediators in the pathogenesis of appendicitis. This review article examines the various types of inflammatory mediators involved, including cytokines, chemokines, and prostaglandins, and discusses their interactions with other cells and molecules in the inflammatory cascade. Our review highlights the complex interplay between inflammatory mediators in the development of appendicitis and their potential implications for diagnosis and treatment of the disease. We discuss the potential for targeted therapies aimed at reducing the production or activity of specific inflammatory mediators, as well as the potential for new diagnostic approaches based on the detection of specific mediators in the blood or other bodily fluids. At the end, the role of inflammatory mediators in appendicitis is an active area of research, and continued investigation is necessary to fully elucidate the mechanisms involved. However, the growing understanding of the complex interactions between these molecules offers new opportunities for the development of targeted therapies and diagnostic tools for this common and potentially serious condition

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Section: Emerging Diagnostic Approaches For Acutementioning

confidence: 99%

Unveiling the Role of Inflammatory Mediators and Gut Microbiome in Appendicitis: Types and Applications in Clinical Scoring

Elwan,

Abdennour,

Akl

et al. 2023

Advanced Gut & Microbiome Research

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show abstract

“…Therefore, we estimate that PITX2 may be involved in the oxidant or regenerative responses in the appendix. If PITX2 is found to affect miR-31 in the appendix, it may help explain its involvement with the in ammation that occurs in appendicitis [37]. A study by Uygar et al analysed mRNA in AA.…”

Section: Overviewmentioning

confidence: 99%

Investigation of VGLL3 and sub-target genes in the aetiology of paediatric acute appendicitis: a prospective case–control study

Tartar

Akdeniz²,

Önalan

et al. 2023

Pediatr Surg Int

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Purpose: Vestigial like family member 3(VGLL3) and its sub-target genes have been determined to have signi cant transcriptomic overlap many autoimmune and in ammatory diseases. In this study, we investigated the role of VGLL3 rs13074432 polymorphism and its sub-target genes in the etiology of acute appendicitis (AA).Methods: 250 patients aged 0-18 years, who underwent appendectomy with the diagnosis of AA (patient group; blood and appendix tissue samples), and 200 healthy children (control group; only blood samples) without appendectomy were included in the prospective case-control study. ELISA method was used for protein level detection of VGLL3 and sub-target genes expression change in obtained tissue samples, and Real-Time Quantitative Reverse Transcription polymerase chain reaction (qRT-PCR) was used for mRNA level detection. Genotyping analyses were performed on DNA samples isolated from blood using TaqMan SNP genotyping test.Results: The frequency of TT variant genotype (p=0.000) and T allele (p=0.002) decreased statistically in the patient group compared to the control. No signi cant correlation was observed between the expression of VGLL3 in the appendiceal tissue and the clinical and demographic data of the patient (p>0.05).Conclusion: This study revealed that the VGLL3 gene and its sub-target genes are associated with the etiology of AA.diet [11] and is associated with in ammation of the appendix and not with colonic in ammation [12]. Vestigial like family member 3 (VGLL3) transcriptional factor is involved in the expression of PITX2, which is shown to be associated with Enterobacteriaceae density in the intestinal tract during in ammation [13,14].The human VGLL3 gene is cytogenetically located at 3p12.1. RS13074432 polymorphism in the VGLL3 gene is located in the 3-untranslated region (3'-UTR) of the gene. Studies have reported that VGLL3 is involved in autoimmune diseases and in ammatory processes [15, 16]. VGLL is activated on the Hippo signalling pathway by connecting to a transcription factor known as Transcriptional enhancer factor domain. The Hippo signalling pathway is involved in basic processes such as organ size regulation, cell proliferation and cell death during organ development [17, 18]. At a genome-wide level, genes regulated by VGLL3 showed a strong association with many autoimmune diseases and had a pronounced transcriptomic overlap with in ammatory processes. Furthermore, studies have reported its association to the nucleus in actively in amed tissue as a transcription factor because of the translocation of VGLL3. VGLL3 appeared to be necessary for optimal response to monocytes and the interferons in salivary gland cells [15, 16,19].The purpose of the present study was to investigate the polymorphic variant rs13074432 of the gene VGLL3, which was known to be associated with autoimmunity and in ammation, and the sub-target genes AJUBA, AMOLT2 and PITX3 for their association with the aetiology of AA. Methods Study designStudy approval was obtained from xxx University Medical Faculty's N...

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Investigation of VGLL3 and sub-target genes in the aetiology of acute appendicitis

Tartar

Akdeniz

Önalan

et al. 2022

Preprint

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Purpose: Vestigial like family member 3(VGLL3) and its sub-target genes have been determined to have significant transcriptomic overlap many autoimmune and inflammatory diseases. In this study, we investigated the role of VGLL3 rs13074432 polymorphism and its sub-target genes in the etiology of acute appendicitis (AA). Methods: 250 patients aged 0-18 years, who underwent appendectomy with the diagnosis of AA (patient group; blood and appendix tissue samples), and 200 healthy children (control group; only blood samples) without appendectomy were included in the prospective case-control study. ELISA method was used for protein level detection of VGLL3 and sub-target genes expression change in obtained tissue samples, and Real-Time Quantitative Reverse Transcription polymerase chain reaction (qRT-PCR) was used for mRNA level detection. Genotyping analyses were performed on DNA samples isolated from blood using TaqMan SNP genotyping test. Results: The frequency of TT variant genotype (p=0.000) and T allele (p=0.002) decreased statistically in the patient group compared to the control. No significant correlation was observed between the expression of VGLL3 in the appendiceal tissue and the clinical and demographic data of the patient (p>0.05). Conclusion: This study revealed that the VGLL3 gene and its sub-target genes are associated with the etiology of AA.

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Circulating microRNAs in acute appendicitis

Cited by 3 publications

References 0 publications

Unveiling the Role of Inflammatory Mediators and Gut Microbiome in Appendicitis: Types and Applications in Clinical Scoring

Unveiling the Role of Inflammatory Mediators and Gut Microbiome in Appendicitis: Types and Applications in Clinical Scoring

Investigation of VGLL3 and sub-target genes in the aetiology of paediatric acute appendicitis: a prospective case–control study

Investigation of VGLL3 and sub-target genes in the aetiology of acute appendicitis

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