Circulating MicroRNAs Expression Profile in Lung Inflammation: A Preliminary Study

Mirra, Davida; Cione, Erika; Spaziano, Giuseppe; Esposito, Rita; Sorgenti, Mario; Granato, Elisabetta; Cerqua, Ida; Gallelli, Luca; Iovino, Pasquale; D’Agostino, Bruno

doi:10.3390/jcm11185446

Cited by 15 publications

(24 citation statements)

References 66 publications

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“…Atopy is an exaggerated IgE-mediated immune response to foreign antigens that makes the immune system more sensitive to common allergic triggers [40]. It is a heterogeneous disease and can be related to other diseases [41][42][43][44][45][46][47][48]. Atopic disorders include various lung inflammatory diseases (e.g., allergic asthma and IgE-mediated components of allergic aspergillosis), allergic rhinitis, conjunctivitis, and atopic dermatitis, in which metabolic abnormalities of the leukotriene pathway play a crucial role [11][12][13][14][15][16][17][18].…”

Section: Discussionmentioning

confidence: 99%

Association between Sex-Related ALOX5 Gene Polymorphisms and Lung Atopy Risk

Mirra

Esposito

Spaziano

et al. 2023

JCM

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Atopy is an exaggerated IgE-mediated immune response to foreign antigens in which metabolic abnormalities of the leukotrienes (LTs) pathway play a crucial role. Recent studies have described sex as a key variable in LT biosynthesis, partly explaining why treatment with anti-LT drugs in atopic subjects leads to better control of symptoms in women. In addition, variability in LT production is often associated with single nucleotide polymorphisms (SNPs) in the arachidonate 5-lipoxygenase (ALOX5) gene, which encodes the leukotriene-synthesizing enzyme machinery, 5-lipoxygenase (5-LO). This study aimed to investigate whether two SNPs of ALOX5 are implicated in sex differences in allergic diseases in a prospective cohort of 150 age- and sex-matched atopic and healthy subjects. Rs2029253 and rs2115819 were genotyped using allele-specific RT-PCR, and serum levels of 5-LO and LTB4 were measured by ELISA. Both polymorphisms are significantly more common in women than in men, and their influences on LT production vary as a function of sex, leading to a decrease in men’s and an increase in women’s serum levels of 5-LO and LTB4. These data represent a new resource for understanding sex-related differences in lung inflammatory diseases, partly explaining why women are more likely to develop allergic disorders than men.

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Section: Discussionmentioning

confidence: 99%

Association between Sex-Related ALOX5 Gene Polymorphisms and Lung Atopy Risk

Mirra

Esposito

Spaziano

et al. 2023

JCM

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“…Many of these pathways have been reported to be closely related to NSCLC. [36][37][38][39] The 20 key signaling pathways are shown in Figure 4e.…”

Section: Go Function and Kegg Pathway Enrichment Analysismentioning

confidence: 99%

The Activity, Composition and Molecular Mechanism of Bothriospermum zeylanicum (Hornem.) Fisch. Et Mey Alleviation of Nonsmall Cell Lung Cancer

Zhao,

Ge,

Liao

et al. 2024

Natural Product Communications

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Objective: To study the effect of the ethyl acetate extract of Bothriospermum zeylanicum (Hornem.) Fisch., the active ingredients and mechanism of Et Mey (EAF) in nonsmall cell lung cancer (NSCLC) were explored. Methods: 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay was used to measure the effect of EAF on the proliferation of A549, HL-60, SMMC-7721, MDA-MB-231, and SW480 tumor cells. The components were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS), and the blood components were retrieved from the constituents absorbed into the blood and metabolites of traditional Chinese medicine (DCABM-TCM) database. The mechanism was determined by network pharmacology and verified by molecular docking. Results: The IC50 values for the proliferation of tumor cell strains were 41.13, 56.72, 36.69, 48.63, and 44.48 μg/mL, respectively. A concentration of 100 μg/mL had the strongest inhibitory effect on A549 cells. A total of 33 chemical components were identified by LC-MS, 7 of which have been reported to have certain anti-NSCLC effects, and a total of 6 blood components were identified using the DCABM-TCM database. Network pharmacology analysis revealed that anti-NSCLC activity mainly involves EGFR tyrosine kinase inhibitor resistance, the PI3K-Akt signaling pathway, the AGE-RAGE signaling pathway in diabetic complications, the HIF-1 signaling pathway and the VEGF signaling pathway. The molecular docking results verified that hesperetin, luteolin, and pinocembrin bind well to the AKT1, IL6, EGFR, BCL2, and CASP3 target proteins. Conclusion: The ethyl acetate fraction of B. zeylanicum (Hornem.) Fisch. Et Mey had obvious ant-non NSCLC activity, which may be related to resistance to EGFR tyrosine kinase inhibitors and the PI3K-Akt signaling pathway in diabetic complications, and its active ingredients may be hesperetin, luteolin, and pinocembrin.

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“…Despite this increased attention, the mechanistic nature remains unclear, making novel biomarkers necessary to improve NSCLC early diagnosis. In previous studies, we have reported that microRNAs (miRNAs) are correlated with several diseases [ 25 , 26 , 27 , 28 ]. MicroRNAs (miRNAs) are a family of short non-coding RNA (approximately 18–22 nucleotides) that act as post-transcriptional regulators through silencing of their downstream target mRNA [ 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

Lung microRNAs Expression in Lung Cancer and COPD: A Preliminary Study

et al. 2023

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Non-small cell lung cancer (NSCLC) is one of the deadliest diseases worldwide and represents an impending burden on the healthcare system. Despite increasing attention, the mechanisms underlying tumorigenesis in cancer-related diseases such as COPD remain unclear, making novel biomarkers necessary to improve lung cancer early diagnosis. MicroRNAs (miRNAs) are short non-coding RNA that interfere with several pathways and can act as oncogenes or tumor suppressors. This study aimed to compare miRNA lung expression between subjects with NSCLC and COPD and healthy controls to obtain the miRNA expression profile by analyzing shared pathways. Lung specimens were collected from a prospective cohort of 21 sex-matched subjects to determine the tissue miRNA expression of hsa-miR-34a-5p, 33a-5p, 149-3p, 197-3p, 199-5p, and 320a-3p by RT-PCR. In addition, an in silico prediction of miRNA target genes linked to cancer was performed. We found a specific trend for has-miR-149-3p, 197-3p, and 34a-5p in NSCLC, suggesting their possible role as an index of the tumor microenvironment. Moreover, we identified novel miRNA targets, such as the Cyclin-Dependent Kinase (CDK) family, linked to carcinogenesis by in silico analysis. In conclusion. this study identified lung miRNA signatures related to the tumorigenic microenvironment, suggesting their possible role in improving the evaluation of lung cancer onset.

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Circulating MicroRNAs Expression Profile in Lung Inflammation: A Preliminary Study

Cited by 15 publications

References 66 publications

Association between Sex-Related ALOX5 Gene Polymorphisms and Lung Atopy Risk

Association between Sex-Related ALOX5 Gene Polymorphisms and Lung Atopy Risk

The Activity, Composition and Molecular Mechanism of Bothriospermum zeylanicum (Hornem.) Fisch. Et Mey Alleviation of Nonsmall Cell Lung Cancer

Lung microRNAs Expression in Lung Cancer and COPD: A Preliminary Study

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