Circulating MicroRNAs as a New Class of Biomarkers of Physiological
Reactions of the Organism to the Intake of Dietary Supplements and
Drugs

Postnikov, P. V.; Efimova, Yulia; Пронина, И. В.

doi:10.2174/2211536611666220422123437

Cited by 4 publications

(5 citation statements)

References 109 publications

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“…Таким образом, изменения гематологических параметров не могут выступать в качестве косвенных показателей применения ксенона. Известно, что прием различных фармакологических средств и препаратов влияет на уровни циркулирующих в плазме микроРНК [19,26]. В нашем случае изменением экспрессии более половины из 84 входящих в панель микроРНК можно было пренебречь, а изменения экспрессии некоторых микроРНК не были достоверными (p > 0,05).…”

Section: Discussionunclassified

“…МикроРНК выделяли из 2 мл плазмы при помощи набора PAXgene Blood miRNA Kit по протоколу производителя с некоторыми модификациями (подробно описано Postnikov и соавт. [24]). МикроРНК смывали с колонок двумя объемами по 40 мкл деионизованной воды.…”

Section: выделение микрорнк из образцов плазмы кровиunclassified

Section: Introductionunclassified

“…Известно, что изменения профиля экспрессии ми-кроРНК могут выступать в качестве ранней реакции организма на физиологический или патологический стимул и быть даже более чувствительными, чем биохимические маркеры [19,20]. МикроРНК детектируются практически во всех биологических жидкостях [21] и стабильны после многочисленных циклов замораживания-оттаивания [19], что упрощает процесс отбора и требования по хранению образцов, являясь важным преимуществом для целей допинг-контроля. В данном исследовании предлагается ранее не использовавшийся в практике работы антидопинговых лабораторий подход, основанный на изменении уровней экспрессии специфичных микроРНК после проведения курса ингаляций ксеноновой смесью Xe/O 2 25:75 (v/v).…”

Section: Introductionunclassified

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MiR-378a-3p and miR-491-5p as markers of xenon abuse in doping control

Postnikov,

Ordzhonikidze,

Khanferyan

et al. 2024

jour

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Xenon stimulates the synthesis of the hormone erythropoietin, which leads to improved oxygen supply to tissues, increased endurance and can be used by athletes to gain an undue advantage in competitions. The World Anti-Doping Agency (WADA) banned its use. The determination of xenon in biological fluids, in particular, in blood plasma samples, is difficult due to the narrow detection window. Its indirect detection is possible by changing in some blood parameters during a clinical analysis (RET%, HGB, HCT, etc.), however, this analysis is nonspecific and the use of other erythropoiesis-stimulating agents can lead to similar changes.Aims: The aim of the study was to search for long-term microRNA markers, the expression of which is specific and markedly altered by inhaled xenon.Methods: Quantitative real-time PCR was performed on CFX96 Bio-Rad analyser using miRCURY® LNA® miRNA SYBR® Green PCR Kit and panels for studying the expression profiles of mature microRNAs of the hypoxia signaling pathway miRCURY LNA™ miRNA Focus Panel.Results: Based on statistical data analysis, it was found that the expression of hsa-miR-378a-3p and hsa-miR-491-5p in blood plasma increases significantly (more than 70 times) when xenon inhalations are used as an erythropoiesis stimulator. Measurement of hematological parameters before and after inhalation showed no significant changes that could affect endurance or give competitive advantages.Conclusion: The evaluated difference in microRNA expression levels before and after administration of the xenon mixture (Xe/O2) makes hsa-miR-378a-3p and hsa-miR-491-5p potential candidates for the role of long-term markers of xenon abuse.

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Section: Discussionunclassified

Section: выделение микрорнк из образцов плазмы кровиunclassified

Section: Introductionunclassified

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MiR-378a-3p and miR-491-5p as markers of xenon abuse in doping control

Postnikov,

Ordzhonikidze,

Khanferyan

et al. 2024

jour

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“…The abuse of pharmacological active substance which improve athletic performance may alter the expression of specific genes involved in muscle and bone metabolism by epigenetic mechanisms, such as DNA methylation, histone tails modifications and ncRNA production [188]. The presence of ncRNAs in biological fluids such as blood or saliva, and their adapted production to lifestyle, dietary supplementation or drug abuse make this new class of RNAs a potential powerful tool for the detection of athletes health status and for doping investigation [189].…”

Section: Upcoming Epigenetic Markers In Dopingmentioning

confidence: 99%

Molecular and Epigenetic Aspects of Opioid Receptors in Drug Addiction and Pain Management in Sport

Mazzeo

Meccariello

Guatteo

2023

IJMS

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Opioids are substances derived from opium (natural opioids). In its raw state, opium is a gummy latex extracted from Papaver somniferum. The use of opioids and their negative health consequences among people who use drugs have been studied. Today, opioids are still the most commonly used and effective analgesic treatments for severe pain, but their use and abuse causes detrimental side effects for health, including addiction, thus impacting the user’s quality of life and causing overdose. The mesocorticolimbic dopaminergic circuitry represents the brain circuit mediating both natural rewards and the rewarding aspects of nearly all drugs of abuse, including opioids. Hence, understanding how opioids affect the function of dopaminergic circuitry may be useful for better knowledge of the process and to develop effective therapeutic strategies in addiction. The aim of this review was to summarize the main features of opioids and opioid receptors and focus on the molecular and upcoming epigenetic mechanisms leading to opioid addiction. Since synthetic opioids can be effective for pain management, their ability to induce addiction in athletes, with the risk of incurring doping, is also discussed.

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Determination of possible microRNA-markers of cobalt abuse by real-time qPCR using hypoxia signaling pathway panels

Postnikov

Radus

Efimova

et al. 2023

Fine Chem. Technol.

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Objectives. Cobalt mimics the state of hypoxia to prevent degradation of the alpha subunit of hypoxia-inducible factor, resulting in an increase in blood oxygen capacity and endurance. Athletes can use this property to gain competitive advantage. Nowadays, direct methods of inductively coupled plasma mass spectrometry and liquid chromatography-tandem mass spectrometry are used to determine total cobalt levels in the body. However, the World Anti-Doping Agency is yet to establish a maximum allowable threshold concentration of this element in biofluids. The lack of clear identification criteria complicates the interpretation of the obtained results for the purposes of doping control. In this regard, the present work proposes a new approach for the indirect determination of possible cobalt abuse based on changes in the expression levels of miRNAs involved in the regulation of hypoxia signaling pathways. Here, the aim is to identify possible microRNA markers whose expression does not depend on exercise-induced hypoxia, but changes markedly when taking cobalt preparations.Methods. MicroRNA isolation was performed from blood plasma samples using the PAXgene Blood miRNA Kit. Quantitative real-time polymerase chain reaction (PCR) was performed on CFX96 Bio-Rad (USA) analyzer using miScript® SYBR® Green PCR Kits and panels for studying the expression profiles of mature microRNAs of the hypoxia signaling pathway miScript® miRNA PCR Array.Results. Based on the statistical analysis of the data, it was found that the expression of hsa-miR-15b-5p in the blood plasma of the subjects does not depend on physical activity, but increases when taking cobalt preparations.Conclusions. The difference in expression levels during anaerobic exercise-induced hypoxia and cobalt-induced hypoxia makes hsa-miR-15b-5p a potential candidate to be a marker of erythropoiesis-stimulating agent abuse.

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Circulating MicroRNAs as a New Class of Biomarkers of Physiological Reactions of the Organism to the Intake of Dietary Supplements and Drugs

Cited by 4 publications

References 109 publications

MiR-378a-3p and miR-491-5p as markers of xenon abuse in doping control

MiR-378a-3p and miR-491-5p as markers of xenon abuse in doping control

Molecular and Epigenetic Aspects of Opioid Receptors in Drug Addiction and Pain Management in Sport

Determination of possible microRNA-markers of cobalt abuse by real-time qPCR using hypoxia signaling pathway panels

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