Circulating microRNAs and ovarian reserve disorders

Abstract

between November 2013 and March 2014. Total 2085 patients underwent an ultrasound scan (Voluson 730, GE Healthcare, Istanbul, Turkey) by one of the two physicians (C.P. or Z.D.) between days 1 and 12 of the menstrual cycle. Inclusion criteria were (1) age 20 -50, (2) regular menstrual bleeding between 21 to 35 days, and (3) optimal visualization of both ovaries. The exclusion criteria were (1) hirsutism or menstrual irregularity, (2) any hormonal drug or oral contraceptive pill use within the last 6 months, (3) history of endometrioma cystectomy or detection of current endometrioma at the time of ultrasonography, and (4) pregnancy. The fertility status was not a criterion while deciding to include or exclude. The sum of both ovaries counts produced the final AFC. The LMS method was preferred to produce the smoothed centile curves of antral follicle count by age. For each set of percentile curves, the initial smoothing methods were applied to 10th, 25th, 50th, 75th and 90th percentiles. For the comparison of AFC across the age groups, statistical analyses were performed by SPSS v21.0. Significance value was set at 0.05. Approval from institutional review board was obtained.RESULTS: Of the 2805 women that had been examined with ultrasonography during the study period, 381 were appropriate for the final evaluation according to inclusion and exclusion criterion (Table 1). The mean decrease of AFC in each year was 0.41. Among the age groups, there were no statistical significance between 20-24, 25-29 and 30-34, whereas decline in AFC was obvious after 35 years.CONCLUSIONS: The documentation of AFC nomogram might present several benefits in theory. Initially, marking the current AFC of a given woman across the nomogram might predict a woman's total fertility potential not only for assisted reproduction technologies (Polyzos, 2014) but also for the chance of natural conception and pregnancy outcome. In conclusion, comparison of our data and others suggest that AFC nomogram from different reports and countries present similarity regarding the decline by age. Those nomograms might be used to define age specific thresholds according to the clinical conditions.

Dissecting the role of micro-RNAs as a diagnostic marker for polycystic ovary syndrome: a systematic review and meta-analysis

“…A few studies reported that polymorphisms in miRNA may also change miRNA levels, thus altering metabolic parameters. A better understanding of the regulation of miRNAs may offer a theoretical foundation for ovarian diseases (12). This comprehensive review specifically looks at the aberrant expression of a multitude of microRNAs implicated in PCOS.…”