Circulating microRNA expression and their target genes in deep vein thrombosis

Abstract

Background:Clinically, d-dimer is the only established biomarker for the diagnosis of deep vein thrombosis (DVT). However, low specificity discounts its diagnostic value. Several publications have illustrated the differentially expressed circulating microRNAs (miRNAs) and their potential diagnostic values for DVT patients. Therefore, we systematically evaluated present researches and further performed bioinformatics analysis, to provide new insights into the diagnosis and underlying mechanisms of miRNAs in DVT.Methods:Databases PubMed, Web of Science, and Embase were searched from January 2000 to April 2017. Articles on circulating miRNAs expression in DVT were retrieved and reference lists were handpicked. Bioinformatics analysis was conducted for further evaluation.Results:Eventually, the eligibility criteria for inclusion in this study were met by 3 articles, which consisted of 13 specially expressed miRNAs and 149 putative target genes. Two representative KEGG pathways, vascular endothelial growth factor and phosphatidylinositol 3’-kinase (PI3K)-Akt signaling pathway, seemed to participate in the regulatory network of thrombosis.Conclusions:Despite the potential diagnostic value and regulation effect, the results of circulating miRNAs used as biomarkers for DVT are not so encouraging. More in-depth and larger sample investigations are needed to explore the diagnostic and therapeutic values of miRNAs for DVT.

Overexpressed microRNA‐103a‐3p inhibits acute lower‐extremity deep venous thrombosis via inhibition of CXCL12

“…Through literature retrieval, we found five downregulated miRNAs in LEDVT: miR‐136‐5p, miR‐103a‐3p, miR‐191‐5p, miR‐301a‐3p, and miR‐199b‐3p . RT‐qPCR was then performed to detect their expression in the plasma of 90 acute LEDVT patients and 20 normal controls.…”

“…Through literature retrieval, we found five downregulated miRNAs in LEDVT: miR-136-5p, miR-103a-3p, miR-191-5p, miR-301a-3p, and miR-199b-3p. 19 RT-qPCR was then performed to detect their expression in the plasma of 90 acute LEDVT patients and 20 normal controls. Expression of miR-136-5p, miR-103a-3p, miR-191-5p, miR-301a-3p, and miR-199b-3p were all decreased in acute LEDVT, among which miR-103a-3p presented with the lowest expression (p < .05) (Figure 1a).…”

Transcriptome analysis of Klf15‑mediated inhibitory functions in a mouse deep venous thrombosis model

“…The data and analyses in the current study suggest that pathways including TNF, PI3K-Akt, IL-17, Jak-STAT, NF-κB, and MAPK should be considered, as such pathways were correlated with the formation of thrombi according to the KEGG enrichment analysis of the DEGs between the DVT and Blank groups. Previous studies (22,23) have reported that MAPK pathways are related to vascular endothelial venous thrombosis and our colleagues have suggested that resveratrol may exert an in vitro antithrombotic activity by inactivating MAPK signaling pathways (24). Moreover, KEGG analysis of the comparison of the Klf15-siRNA group and the Klf15-NC group indicated that PI3K-Akt play a central role in the regulatory pathway involved in DVT formation.…”

A novel rationale for targeting FXI: Insights from the hemostatic miRNA targetome for emerging anticoagulant strategies

“…These include hemophilia A (Sarachana, et al, 2015), aberrant coagulation in sepsis (H. J. , venous thrombosis (Rodriguez-Rius, Lopez, Martinez-Perez, Souto, & Soria, 2020; X. Xiang, et al, 2019), venous thrombo-embolism (Hembrom, et al, 2020;Jiang, et al, 2017), arterial thrombosis (J. Lin, et al, 2016), acute pulmonary embolism (Guo, et al, 2014;Kessler, et al, 2016;Liu, Kang, & Liu, 2018;Xiao, et al, 2011;X.…”