Circulating microRNA: a novel potential biomarker for early diagnosis of Intracranial Aneurysm Rupture a case control study

Jin, Hengwei; Li, Conghui; Ge, Huijian; Jiang, Yuhua; Li, Youxiang

doi:10.1186/1479-5876-11-296

Cited by 48 publications

(42 citation statements)

References 23 publications

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“…Rupture of intracranial aneurysms (IAs) is a major cause of hemorrhagic stroke, especially SAH (Krings et al, 2011). Changes in circulating miRNA profiles were shown to correlate with the presence of IAs (Jin et al, 2013; Li et al, 2014b). Jin et al (2013) showed altered expression of 86 miRNAs (69 up- and 17 downregulated) in the serum of patients with aneurysms compared to control subjects.…”

Section: Micrornas As Stroke Biomarkersmentioning

confidence: 99%

“…Changes in circulating miRNA profiles were shown to correlate with the presence of IAs (Jin et al, 2013; Li et al, 2014b). Jin et al (2013) showed altered expression of 86 miRNAs (69 up- and 17 downregulated) in the serum of patients with aneurysms compared to control subjects. One of the miRNAs identified to be upregulated in this study (miR-25) was also shown by Li et al (2014b).…”

Section: Micrornas As Stroke Biomarkersmentioning

confidence: 99%

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Non-coding RNAs and neuroprotection after acute CNS injuries

Chandran

Mehta

2017

Neurochemistry International

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Accumulating evidence indicates that various classes of non-coding RNAs (ncRNAs) including microRNAs (miRNAs), PIWI-interacting RNAs (piRNAs) and long non-coding RNAs (lncRNAs) play important roles in normal state as well as the diseases of the CNS. Interestingly, ncRNAs have been shown to interact with messenger RNA, DNA and proteins, and these interactions could induce epigenetic modifications and control transcription and translation, thereby adding a new layer of genomic regulation. The ncRNA expression profiles are known to be altered after acute CNS injuries including stroke, traumatic brain injury and spinal cord injury that are major contributors of morbidity and mortality worldwide. Hence, a better understanding of the functional significance of ncRNAs following CNS injuries could help in developing potential therapeutic strategies to minimize the neuronal damage in those conditions. The potential of ncRNAs in blood and CSF as biomarkers for diagnosis and/or prognosis of acute CNS injuries has also gained importance in the recent years. This review highlighted the current progress in the understanding of the role of ncRNAs in initiation and progression of secondary neuronal damage and their application as biomarkers after acute CNS injuries.

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Section: Micrornas As Stroke Biomarkersmentioning

confidence: 99%

Section: Micrornas As Stroke Biomarkersmentioning

confidence: 99%

Non-coding RNAs and neuroprotection after acute CNS injuries

Chandran

Mehta

2017

Neurochemistry International

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“…This makes miRNA an important regulatory molecule, and it is known to play a role a variety of pathologies. 6–12 There are only a handful of prior studies that have profiled miRNA expression in either human intracranial aneurysms or relevant animal models, 13–15 alongside a small number of studies which focus on the roles of particular miRNA. 16, 17 Already these studies are revealing potential biomarkers 13 and the importance of these regulatory molecules.…”

Section: Introductionmentioning

confidence: 99%

RNA-Sequencing Analysis of Messenger RNA/MicroRNA in a Rabbit Aneurysm Model Identifies Pathways and Genes of Interest

Holcomb

Ding

Dai

et al. 2015

AJNR Am J Neuroradiol

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Background and Purpose Rabbit aneurysm models are used for the testing of embolization devices and elucidating the mechanisms of human intracranial aneurysm growth and healing. We used RNA-sequencing technology to identify genes relevant to induced rabbit aneurysm biology and to identify genes and pathways of potential clinical interest. This process included sequencing microRNAs, which are important regulatory noncoding RNAs. Materials and Methods Elastase-induced saccular aneurysms were created at the origin of the right common carotid artery in 6 rabbits. Messenger RNA and microRNA were isolated from the aneurysm and from the control left common carotid artery at 12 weeks and processed by using RNA-sequencing technology. The results from RNA sequencing were analyzed by using the Ingenuity Pathway Analysis tool. Results A total of 9396 genes were analyzed by using RNA sequencing, 648 (6.9%) of which were found to be significantly differentially expressed between the aneurysms and control tissues (P<.05; false-discovery rate<0.01; fold change, >2 or <.5). Of these genes, 614 were mapped successfully, 143 were down-regulated, and 471 were up-regulated in the aneurysms as compared with controls. Using the same criteria for significance, 3 microRNAs were identified as down-regulated and 5 were identified as up-regulated. Pathway analysis associated these genes with inflammatory response, cellular migration, and coagulation, among other functions and pathologies. Conclusion RNA-sequencing analysis of rabbit aneurysms revealed differential regulation of some key pathways, including inflammation and antigen presentation. ANKRD1 and TACR1 were identified as genes of interest in the regulation of matrix metalloproteinases.

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“…Prior studies have noted that miRNAs play a critical role in vascular diseases through regulating VSMCs and endothelial cell functions, such as proliferation, migration, apoptosis, and synthesis/secretion of ECM proteins [8-10]. Our previous microarray analyses have identified 86 serum miRNAs with significantly different expression between normal subjects and aneurysm patients, suggesting that dysregulation of miRNAs might participate in the modulation of basic physiological and pathological processes involved in IA and may be a warning sign for rupture [6]. These aberrantly expressed miRNAs are related to apoptosis, vascular remodelling, ECM degradation, inflammation and immune responses in IA walls.…”

Section: Introductionmentioning

confidence: 99%

MiR-29b Downregulation Induces Phenotypic Modulation of Vascular Smooth Muscle Cells: Implication for Intracranial Aneurysm Formation and Progression to Rupture

Zhao

Zhang

et al. 2017

Cell Physiol Biochem

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Background/Aims: Our previous microarray results identified numerous microRNAs (miRNAs), including miR-29b, that were differentially expressed in the serum of intracranial aneurysm (IA) patients. The current study aimed to investigate whether miR-29b downregulation in IA could promote the phenotypic modulation of vascular smooth muscle cells (VSMCs) involved in the pathogenesis of aneurysm by activating ATG14-mediated autophagy. Methods: First, the levels of miR-29b and autophagy related genes (ATGs) between IA patients and normal subjects were compared. Next, we modified the level of miR-29b via lentivirus particles in the VSMCs and examined the effects of miR-29b on proliferation, migration, and phenotypic modulation of VSMCs from a contractile phenotype to a synthetic phenotype, as well as the levels of autophagy. Finally, the binding of miR-29b to the 3’UTR of ATG14 mRNA and its effects on ATG14 expression were analysed by a luciferase reporter assay and Western blot, respectively. Results: The level of miR-29b was decreased, and autophagy markers were increased in the IA patients compared to that of the normal subjects. Knockdown of miR-29b significantly promoted VSMCs proliferation and migration and, more importantly, induced the phenotypic modulation associated with autophagy activation, whereas miR-29b overexpression showed the opposite effects. The luciferase reporter assay demonstrated that ATG14 was a functional target gene of miR-29b. Notably, knockdown of ATG14 by siRNA apparently abrogated miR-29b inhibition-mediated phenotypic modulation. Conclusion: Downregulation of miR-29b induced VSMCs phenotypic modulation by directly activating ATG14-mediated autophagy, which is associated with the formation, growth and rupture of IAs.

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Circulating microRNA: a novel potential biomarker for early diagnosis of Intracranial Aneurysm Rupture a case control study

Cited by 48 publications

References 23 publications

Non-coding RNAs and neuroprotection after acute CNS injuries

Non-coding RNAs and neuroprotection after acute CNS injuries

RNA-Sequencing Analysis of Messenger RNA/MicroRNA in a Rabbit Aneurysm Model Identifies Pathways and Genes of Interest

MiR-29b Downregulation Induces Phenotypic Modulation of Vascular Smooth Muscle Cells: Implication for Intracranial Aneurysm Formation and Progression to Rupture

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