Circulating levels of total angiopoietin-2 and the soluble Tie-2 receptor in women during ovarian stimulation and early gestation

Molskness, Theodore A.; Stouffer, Richard L.; Burry, Kenneth A.; Gorrill, Marsha J.; Lee, David M.; Patton, Phillip E.

doi:10.1016/j.fertnstert.2006.03.045

Cited by 6 publications

(9 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pooled samples were included in assays to confirm accuracy. Inter- and intra-assay variations were 18% and 11.5% for ANGPT-1 and 3% and 11% for ANGPT-2 (19), respectively.…”

Section: Materials/methodsmentioning

confidence: 94%

“…Venous blood samples were collected from 8 pregnant, rhesus monkeys from mid-to-late gestation; this interval was chosen based on our earlier data from human pregnancy (19). Pregnant monkeys for blood sampling were provided by the Assisted Reproductive Technologies (ART) Core, ONPRC.…”

Section: Materials/methodsmentioning

confidence: 99%

“…The protocol, involving pituitary down-regulation with GnRH agonist (Lupron; TAP Pharmaceuticals, Deerfield, IL, USA), with a urinary gonadotropin (HMG) and recombinant human FSH mixture started after induced menses to promote multiple follicular development, was similar to that previously described (Gonal F, Serono, Rockland, MA; (19)). Dosage was individualized based on serial ultrasound results and serum estradiol levels.…”

Section: Materials/methodsmentioning

confidence: 99%

“…Whether ANGPTs of ovarian or placental origin circulate in the blood has not been detailed. Based on our preliminary report of circulating ANGPT-2 and its soluble Tie-2 receptor in women during gestation (19), further studies were designed to determine if the ANGPTs are detectable in the circulation of nonhuman primates and women in natural or COS cycles, and whether these levels fluctuate in association with ovarian activity over the course of the reproductive cycle and in pregnancy.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Changes in circulating levels and ratios of angiopoietins during pregnancy but not during the menstrual cycle and controlled ovarian stimulation

Hurliman

Speroff

Stouffer

et al. 2010

Fertility and Sterility

Self Cite

View full text Add to dashboard Cite

OBJECTIVE This study was designed to determine if angiopoietin (ANGPT)-1 and -2 are detectable in the circulation of nonhuman primates and women, and if these levels fluctuate in association with ovarian activity. DESIGN Prospective SETTING National Primate Research Center, medical center and infertility clinic. PATIENTS Adult, female rhesus monkeys; 15 women donating oocytes for infertility treatment. INTERVENTIONS Controlled ovarian stimulation with gonadotropins, removal of the corpus luteum and ovaries, oocyte retrieval and embryo transfer. MAIN OUTCOME MEASURE Circulating levels of ANGPT-1 and ANGPT-2. RESULTS Serum ANGPT-1 and ANGPT-2 levels were detectable and invariant in maintaining an ANGPT1:2 ratio >1 in: (a) macaques over the course of the natural menstrual cycle, during a controlled ovulation protocol and following removal of the corpus luteum or ovaries, and (b) women undergoing controlled ovarian simulation (COS). In contrast, the ANGPT1:2 ratio was markedly decreased (≪1) at mid-to-late gestation in macaques, and in the follicular fluid of women undergoing COS, due to increased levels of ANGPT-2. CONCLUSIONS The ovary and its dominant structures are not major contributors to circulating levels of ANGPT-1 or ANGPT-2. The physiologic importance of the rising levels of ANGPT-2 after the luteal-placental shift in pregnancy is unknown.

show abstract

“…Pooled samples were included in assays to confirm accuracy. Inter- and intra-assay variations were 18% and 11.5% for ANGPT-1 and 3% and 11% for ANGPT-2 (19), respectively.…”

Section: Materials/methodsmentioning

confidence: 94%

Section: Materials/methodsmentioning

confidence: 99%

Section: Materials/methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Changes in circulating levels and ratios of angiopoietins during pregnancy but not during the menstrual cycle and controlled ovarian stimulation

Hurliman

Speroff

Stouffer

et al. 2010

Fertility and Sterility

Self Cite

View full text Add to dashboard Cite

show abstract

“…Adipose tissue, a highly vascular and active endocrine organ, may be a source of angiopoietins and their receptors; and may influence sTie-2 levels 26 . Despite variation by maternal characteristics, previous studies have also described constant serum sTie-2 levels across first trimester 27 , mid pregnancy 10 and a slight increase after 26 weeks gestation 12 .…”

Section: Discussionmentioning

confidence: 91%

Evaluation of first trimester serum soluble endothelial cell-specific tyrosine kinase receptor in normal and affected pregnancies

Schneuer

Nassar

Guilbert

et al. 2014

The Journal of Maternal-Fetal & Neonatal Medicine

View full text Add to dashboard Cite

Short title: sTie-2 on pregnancy outcomesKey words: sTie-2 receptor, first trimester, adverse pregnancy outcomes, serum levels, predictive accuracy 2 ABSTRACT Aims: To assess soluble endothelial cell-specific tyrosine kinase receptor (sTie-2) levels in the first trimester of pregnancy and its association with adverse pregnancy outcomes; and examine the predictive accuracy.Study Design: In this nested case-control study, serum sTie-2 levels were measured in 2,616 women with singleton pregnancies attending first trimester screening in New South Wales, Australia. Multivariate logistic regression models were used to assess the association and predictive accuracy of serum sTie-2 with subsequent adverse pregnancy outcomes.Results: Median (interquartile range) sTie-2 for the total population was 19.6 ng/ml (13.6-26.4). Maternal age, weight, and smoking status significantly affected sTie-2 levels. There was no difference in serum sTie-2 between unaffected and women with adverse pregnancy outcomes. After adjusting maternal and clinical risk factors, low sTie-2 (<25 th centile) was associated with preeclampsia (Adjusted odds ratio: 1.61; 95%CI: 1.01-2.57), however, the accuracy of sTie-2 in predicting preeclampsia was not different from chance (AUC=0.54; P=0.08) and does not add valuable predictive information to maternal and clinical risk factors. Conclusions:Our findings suggest that low sTie-2 levels are associated with preeclampsia, however, it does not add valuable information to clinical and maternal risk factor information in predicting preeclampsia or any other adverse pregnancy outcomes.3

show abstract

Involvement of the ANGPTs/Tie-2 system in ovarian hyperstimulation syndrome (OHSS)

Scotti

Abramovich

Pascuali

et al. 2013

Molecular and Cellular Endocrinology

View full text Add to dashboard Cite

Circulating levels of total angiopoietin-2 and the soluble Tie-2 receptor in women during ovarian stimulation and early gestation

Cited by 6 publications

References 8 publications

Changes in circulating levels and ratios of angiopoietins during pregnancy but not during the menstrual cycle and controlled ovarian stimulation

Changes in circulating levels and ratios of angiopoietins during pregnancy but not during the menstrual cycle and controlled ovarian stimulation

Evaluation of first trimester serum soluble endothelial cell-specific tyrosine kinase receptor in normal and affected pregnancies

Involvement of the ANGPTs/Tie-2 system in ovarian hyperstimulation syndrome (OHSS)

Contact Info

Product

Resources

About