Circulating Inflammatory Endothelial Cells Contribute to Endothelial Progenitor Cell Dysfunction in Patients with Vasculitis and Kidney Involvement

Holmén, Carolina; Elsheikh, Elzafir; Stenvinkel, Peter; Qureshi, Abdul Rashid; Pettersson, E; Jalkanen, Sirpa; Sumitran–Holgersson, Suchitra

doi:10.1681/asn.2005040347

Cited by 96 publications

(97 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…12 K An increase in the number of CECs has been used for assessment of vascular damage and it seems to be a marker of disease activity in recent years. 5,7 What this study adds K This study shows that CECs as a marker of endothelial injury were significantly higher in patients with preeclampsia than in hypertensive patients, healthy pregnants and normal controls. K At the same time, plasma homocysteine level was increased in preeclamptic patients in concordance with the CECs.…”

Section: Conflict Of Interestmentioning

confidence: 70%

“…[5][6][7][8] In their study, Koc et al 14 reported that an increase in CECs in haemodialysis patients seems to predict the development of cardiovascular and vascular events, and to be independent of other known markers of inflammation or endothelial dysfunction. Preeclampsia is a disease in which impaired maternal endothelial function seems to be central in the pathogenesis and has been observed to persist for several years following the preeclamptic pregnancy.…”

Section: Discussionmentioning

confidence: 99%

“…4 An increased number of circulating endothelial cells (CECs) has previously been reported after the onset of various diseases that are associated with endothelial injury, including myocardial infarction, septic shock, sickle cell crisis, antineutrophil cytoplasmic autoantibody (ANCA)-associated small vessel vasculitis, thrombotic thrombocytopenic purpura (TTP), systemic lupus erythematosus and Behçet's disease. [5][6][7][8] An increase in the number of CECs has been used for assessment of vascular damage and it seems to be a marker of disease activity in recent years. 6,8 Hyperhomocysteinemia is now widely recognized as an independent risk factor for cardiovascular disease -homocysteine level was found to be increased and was associated with cellular fibronectin in preeclampsia.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Circulating endothelial cells in preeclampsia

et al. 2007

View full text Add to dashboard Cite

Endothelial dysfunction plays an important role in the pathogenesis of preeclampsia. Increased number of circulating endothelial cells (CECs) have previously been reported after various diseases associated endothelial injury. The aim of this study was to evaluate the CECs in patients with preeclampsia and to demonstrate any association between CECs and homocysteine, which is another marker of vascular injury. The study included 20 preeclamptic, 15 hypertensive women, 15 healthy pregnant and 15 healthy non-pregnant women. All subjects had normal renal function. Systolic and diastolic blood pressures, serum homocysteine levels were measured. To isolate CECs, peripheral blood was first incubated with anti-CD-146 antibody and subsequently conjugated to immunomagnetic beads. Cells were stained with acridine and counted. Preeclamptic patients had elevated numbers of CECs (13.275.2 cells/ml) compared with hypertensive patients (6.970.8 cells/ml), healthy pregnants (5.271.4 cells/ml) and non-pregnant controls (4.071.8 cells/ml), (Po0.0001). Serum homocysteine level in preeclamptic patients (9.572.8 lmol/l) was significantly higher compared with healthy pregnants (6.070.6 lmol/l), was not different from hypertensive patients (11.572.3 lmol/l, P40.05), but it was lesser compared with non-pregnant controls (12.273.3 lmol/l, Po0.0001). Also, significant correlation between CECs and systolic blood pressure (Po0.0001, r ¼ 0.63), diastolic blood pressure (Po0.0001, r ¼ 0.64) and serum homocysteine (Po0.01, r ¼ 0.55) levels were found in preeclamptic patients. CECs as a marker of endothelial injury were significantly higher in patients with preeclampsia than in hypertensive patients, healthy pregnants and normal controls. Further studies are needed for the prognostic and potential importance of CECs in preeclampsia.

show abstract

Section: Conflict Of Interestmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Circulating endothelial cells in preeclampsia

et al. 2007

View full text Add to dashboard Cite

show abstract

“…In contrast, however recent studies showed significant reduced levels of numbers of EPC-CFU during the active phase of the disease and suggested that impairment of EPC function was mediated in part by inflammatory circulating endothelial cells (Holmén et al, 2005).…”

Section: Epcs In Vasculitismentioning

confidence: 88%

Endothelial progenitor cells: Are they displaying a function in autoimmune disorders?

Ferrante

Guggino

Liberto

et al. 2016

Mechanisms of Ageing and Development

View full text Add to dashboard Cite

a b s t r a c tEndothelial Progenitor Cells (EPCs) are bone marrow derived cells able to differentiate in mature endothelial cells (EC) contributing to the generation of new vessels, connecting to fibronectin, and forming colonies and/or colony forming units.Since circulating EPCs can be actively considered part of endothelial damage in several cardiovascular diseases and autoimmune disorders the possibility to have a measure for endothelium damage should be considered of interest to predict the patient out-come. At the same time the EPCs proliferative and regenerative role could be considered for therapeutic applications.

show abstract

“…Automated systems have been described but these are costly 25 In addition, there is considerable discrepancy in these numbers between different groups that employ FACS. It is remarkable that most if not all investigators using immunomagnetic isolation enumerate in the range of 10 CEC/ml blood in healthy individuals while those using FACS report cell numbers in the thousands per ml with a fairly broad range 10 : Holmen and colleagues measured a mean of 50 CEC/ml in healthy controls 26 , Mancuso et al counted 1,200 CEC/ml of rested cells 36 and Jacques N et al 6.5 CEC/ml 37 . Two groups compared CEC counts measured with both methods in the same populations.…”

Section: Cec and Vasculitis: Immunomagnetic Isolation And Facs In Commentioning

confidence: 99%