Circulating heat-shock protein 70 is associated with postoperative infection and organ dysfunction after liver resection

Kimura, Fumio; Itoh, Hiroshi; Ambiru, Satoshi; Shimizu, Hiroaki; Togawa, Akira; Yoshidome, Hiroyuki; Ohtsuka, Masayuki; Shimamura, Fumihiko; Kato, Atsushi; Nukui, Yuji; Miyazaki, Masaru

doi:10.1016/j.amjsurg.2003.08.029

Cited by 61 publications

(50 citation statements)

References 31 publications

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“…Hsp70 has been known to act not only as an intracellular chaperon, but also as a secretory protein (Chan et al, 1999;Asea et al, 2000;Guzhova et al, 2001;Somersan et al, 2001;Dybdahl et al, 2002;Njemini et al, 2003;Hunter-Lavis et al, 2004;Kimura et al, 2004;Njemini et al, 2004). Secretion of Hsp70 from a variety of cells in response to cellular stress has been widely reported.…”

Section: Discussionmentioning

confidence: 99%

“…However, it has been suggested that Hsp70 is released extracellularly under normal cell culture conditions (Hightower et al, 1989;Broquet et al, 2003;HunterLavis et al, 2004), and is present in circulation of normal individuals, and its circulating level is increased after open heart surgery or in patients with cardiovascular diseases including atherosclerosis (Chan et al, 1999;Dybdahl et al, 2002). Furthermore, plasma concentrations of Hsp70 were shown to be associated significantly with postoperative infection (Kimura et al, 2004). These results suggest that Hsp70 can be released into extracellular spaces and may be involved in postoperative inflammatory responses and in the pathogenesis of postoperative organ dysfunction.…”

Section: Introductionmentioning

confidence: 99%

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Release of heat shock protein 70 (Hsp70) and the effects of extracellular Hsp70 on matric metalloproteinase-9 expression in human monocytic U937 cells

Lee

Kim²,

Kim³

et al. 2006

Exp Mol Med

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Heat shock protein 70 (Hsp70) release and its effects on pro-inflammatory cytokine production have been controversial. In this study, we investigated whether Hsp70 could be released from monocytes and activates matrix metalloproteinase-9 (MMP-9) gene expression. Hsp70 overexpression in human monocytic cell line U937 was found to increase PMAinduced MMP-9 expression and enhance cell motility. Hsp70 cDNA transfectants released Hsp70 protein into culture supernatants, and a part of released Hsp70 subsequently was bound to the surface of U937 cells. Addition of culture medium containing the extracelluar Hsp70 led to an increase not only in proMMP-9 secretion, but also the invasiveness of U937 cells through Matrigel or human umbilical vascular endothelial cells (HUVEC) in vitro. Immunodepletion of Hsp70 abolished its effect on MMP-9 expression. The released Hsp70 activated nuclear factor kappa B (NF-κB) and activating protein-1 (AP-1), which led to the activation of MMP-9 transcription. Taken together, these results suggest that extracellular Hsp70 induces the expression of MMP-9 gene through activation of NF-κB and AP-1.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Release of heat shock protein 70 (Hsp70) and the effects of extracellular Hsp70 on matric metalloproteinase-9 expression in human monocytic U937 cells

Lee

Kim²,

Kim³

et al. 2006

Exp Mol Med

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“…For example, increased extracellular Hsp70 levels are noted following strenuous exercise (180,181,(204)(205)(206)(207)(208), following cardio-pulmonary bypass in both adults (209,210) and children (Wheeler, unpublished data), in elderly adults (211,212), and in children with acute lung injury (Wheeler, unpublished data). In addition, increased extracellular Hsp70 concentrations correlate with worse outcome in a variety of inflammatory disease processes, including liver disease (213), coronary artery disease (214)(215)(216)(217), traumatic brain injury secondary to child abuse (218), pre-eclampsia (219), sickle cell disease vasoocclusive crisis (220), septic shock (221), and traumatic brain injury (222). Conversely, increased extracellular Hsp70 levels correlated with improved outcome following multiple trauma in adults (141).…”

Section: Stress Proteins and Innate Immunitymentioning

confidence: 99%

Heat shock response and acute lung injury☆

Wheeler

Wong

2007

Free Radical Biology and Medicine

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All cells respond to stress through the activation of primitive, evolutionarily conserved genetic programs that maintain homeostasis and assure cell survival. Stress adaptation, which is known in the literature by a myriad of terms, including tolerance, desensitization, conditioning, and reprogramming, is a common paradigm found throughout nature, in which a primary exposure of a cell or organism to a stressful stimulus (e.g., heat) results in an adaptive response by which a second exposure to the same stimulus produces a minimal response. More interesting is the phenomenon of cross-tolerance, by which a primary exposure to a stressful stimulus results in an adaptive response whereby the cell or organism is resistant to a subsequent stress that is different from the initial stress (i.e. exposure to heat stress leading to resistance to oxidant stress). The heat shock response is one of the more commonly described examples of stress adaptation and is characterized by the rapid expression of a unique group of proteins collectively known as heat shock proteins (also commonly referred to as stress proteins). The expression of heat shock proteins is well described in both whole lungs and in specific lung cells from a variety of species and in response to a variety of stressors. More importantly, in vitro data, as well as data from various animal models of acute lung injury, demonstrate that heat shock proteins, especially Hsp27, Hsp32, Hsp60, and Hsp70 have an important cytoprotective role during lung inflammation and injury.

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“…Hücre koruyucu fonksiyonları olduğu bilinmektedir ve gebe olmayan, sağlıklı bireylerde de periferal dolaşımda HSP70 saptanmıştır (2) . Periferal ve renal vasküler hastalıkta (3) , egzersiz ile (4) , akut enfeksiyonda (5) , konjestif kalp yetmezliğinde (6) , septik şokta (7) , cerrahi sonrası (8) ve myokard enfaktüsünde (9) periferal dolaşımaki HSP70 düzeyi-nin arttığı saptanmıştır.…”

Section: Introductionunclassified

Heat Shock Protein 70 Levels In Normal Pregnancy And Preeclampsia

Pulur¹,

Karaca²,

Karaca³

2017

Terh

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ÖZAmaç: Hafif preeklamptik hastalar, şiddetli preeklamptik hastalar ve normotansif gebelerden seçilmiş kontrol grubunda, serum ısı şok protein 70 (HSP70) konsantrasyonlarının farklı olup olmadığının araştırılması. Yöntem: Çalışmamızda Atatürk Üniversitesi Tıp Fakültesi Kadın Hastalıkları ve Doğum Kliniğine başvuran 18-45 yaş arası, 20. gestasyonel haftanın üzerindeki monofetüs gebeliğe sahip 30 hafif preeklamptik, 30 şiddetli preeklamptik hasta ile kontrol amaçlı seçilen poliklinkte takip edilmekte olan 30 normotansif sağlıklı gebe, randomize prospektif olarak incelendi. Serum HSP70 konsantrasyonları ELISA yöntemi ile ölçüldü. Bulgular: Çalışma sonuçları incelendiğinde, hafif preeklamptik hastaların HSP70 değerle-ri: 0,634 ng/mL±0,30, şiddetli preeklamptik hastalarda 0,881 ng/mL±0,37, kontrol grubunda ise 0,619 ng/mL±0,27 olarak saptandı. Şiddetli preeklamptik grubun değerlerinde hafif preeklamptik gruba göre anlamlı yükseklik mevcuttu (p=0,01, GA: 0,0467-0,4483), şiddet-li preeklamptiklerin kontrol grubu ile kıyaslanmasında da yine yüksek değerler tespit edildi (p=0.006, GA: 0,0612-0,4628). Korelasyon analizlerinde ise HSP70 düzeyleri ile sistolik kan basıncı (r=0,325, p=0,002); diastolik kan basıncı (r=0,273, p=0.009) ve 24 saatlik idrarda protein (r=0,291, p=0,005) düzeyleri ile anlamlı pozitif korelasyonlar tespit edildi. Sonuç: Çalışmamız sonucunda şiddetli preeklamptik hastaların serum HSP70 düzeyleri hafif preeklamptik hastalara göre ve kontrol grubuna göre daha yüksek saptandı. Preeklampsi ve komplikasyonlarını öngörme konusunda HSP70 önemli bir gösterge olabilir, ancak bu konuda daha kapsamlı çalışmalara gereksinim vardır.Anahtar kelimeler: Gebelik, preeklampsi, ısı şok protein70, HSP 70, HSPA ABSTRACT Objective: The aim of this study was to evaluate whether serum levels of heat shock protein 70 (HSP70) in patients with mild preeclampsia, severe preeclampsia differ when compared with normotensive pregnant controls. Methods: In this randomize prospective study, we enrolled a total of 90 monoparous (30 mild, and 30 severe preeclamptic patients, and 30 normotensive healthy pregnant controls) pregnants aged 18-45 years who passed their 20. gestational weeks, and admitted to the Clinics of Obstetrics and Gynecology of Atatürk University Hospital. Serum HSP70 concentrations were measured by standard ELISA techniques. Results:The mean values of serum HSP70 in mild (0.634±0.30 ng/ml), and severe (0.881±0.37 ng/ml) preeclamptic patients and normotensive controls (0.619±0.27 ng/ml) were measured. Severe preeclamptic patients had statistically significantly higher concentrations of serum HSP70 when compared with mild preeclamptic patients (p=0.01; CI: 0.0467-0.4483), and controls (p=0.006; CI: 0.0612-0.4628). We found that HSP70 concentrations positively correlated with systolic (r=0.325; p=0.002), and diastolic blood pressures (r=0.273; p=0.009) and daily urinary protein (r=0.291; p=0.005) levels. Conclusion: Our study revealed higher serum concentrations of HSP70 in patients with severe preeclampsia when compare...

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Circulating heat-shock protein 70 is associated with postoperative infection and organ dysfunction after liver resection

Cited by 61 publications

References 31 publications

Release of heat shock protein 70 (Hsp70) and the effects of extracellular Hsp70 on matric metalloproteinase-9 expression in human monocytic U937 cells

Release of heat shock protein 70 (Hsp70) and the effects of extracellular Hsp70 on matric metalloproteinase-9 expression in human monocytic U937 cells

Heat shock response and acute lung injury☆

Heat Shock Protein 70 Levels In Normal Pregnancy And Preeclampsia

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