Circulating expression and clinical significance of LncRNA ANRIL in diabetic kidney disease

Abstract

Background Long noncoding RNA ANRIL has been found to be involved in the pathogenesis of diabetic kidney disease (DKD) and is expected to be a new target for prevention of DKD. However, the circulating expression and clinical significance of ANRIL in DKD patients is uncertain. This study aims to explore this issue. Methods The study consisted of 20 healthy controls, 22 T2DM patients (normalbuminuria) and 66 DKD patients (grouped as follows: microalbuminuria, n = 23; macroalbuminuria, n = 22 and renal dysfunction, n = 21). The expressions of ANRIL in peripheral whole blood of all participants were measured by RT-qPCR. Results The expression of ANRIL was significantly up-regulated in DKD patients (microalbuminuria, macroalbuminuria and renal dysfunction groups) than that in healthy control group. ANRIL was also over-expressed in macroalbuminuria and renal dysfunction groups in comparison with normalbuminuria group. ANRIL expression was positively correlated with Scr, BUN, CysC, urine β2-MG and urine α1-MG; while negatively correlated with eGFR in DKD patients. In addition, ANRIL was the risk factor for DKD with OR value of 1.681. The AUC of ANRIL in identifying DKD was 0.922, and the sensitivity and specificity of DKD diagnosis were 83.3% and 90.5%, respectively. Conclusion Our results indicated that highly expressed ANRIL in peripheral blood is associated with progression of DKD. Circulating ANRIL is an independent risk factor of DKD and has a highly predictive value in identifying DKD.

Long noncoding RNAs as potential diagnostic biomarkers for diabetes mellitus and complications: A systematic review and meta‐analysis

“…In addition to the 103 review articles, a total of 246 studies were removed for the following reasons: 120 were animal studies, 108 did not report AUC values, and 18 did not report numerical sensitivity or specificity results. In total, 24 studies ultimately met our eligibility criteria, of which T2DM, DN, and DR were analyzed in 17, 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 5, 5 , 30 , 35 , 36 , 37 and 7 studies, 4 , 28 , 29 , 32 , 37 , 38 , 39 respectively (some articles included more than one of these outcomes). Table 1 provides the specifics of the survey characteristics and summarizes the quality assessment results.…”

Associations between low serum levels of ANRIL and some common gene SNPs in Iranian patients with premature coronary artery disease

“…It has been demonstrated that ANRIL is upregulated in serum samples from patients with multiple malignancies, including glioma and breast cancer 15 , 16 . The higher expression of circulating ANRIL in the peripheral blood and serum of patients with diabetes and ischemic stroke has been reported 17 , 18 . As demonstrated by Holdt and Teupser 12 , atherosclerosis progression is associated with decreased expression of circular ANRIL and increased expression of linear ANRIL .…”

LncRNA as a regulator in the development of diabetic complications

“…Some lncRNAs have a diagnostic role in diabetic complications. ANRIL and MALAT1 are upregulated in patients with DN, as the biomarker for the diagnosis of diabetic kidney disease ( 22 , 26 ). TINCR and HOTAIR are downregulated in serum and myocardial biopsies of patients with DCM and can be used to effectively distinguish patients with DCM from healthy controls ( 58 ; 102 ).…”