Circulating exosome levels in the diagnosis of steroid-induced osteonecrosis of the femoral head

Zhu, Hongyi; Gao, Yanchun; Wang, Yuchen; Zhang, CQ

doi:10.1302/2046-3758.56.bjr-2015-0014.r1

Cited by 28 publications

(20 citation statements)

References 11 publications

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“…As a common disease, osteonecrosis is attracting more and more attention. In order to improve the early diagnosis of GC-induced ONFH or guide clinical trials in GC-induced osteonecrosis, studies of circulating exosomes 24 and microvesicles 25 have aroused widespread interest. Within the past decade, extracellular vehicles (EVs) have emerged as key mediators involved in signal transduction between cells to regulate a diverse range of biological processes in both prokaryotes and eukaryotes 26 .…”

Section: Introductionmentioning

confidence: 99%

Exosomes derived from human platelet-rich plasma prevent apoptosis induced by glucocorticoid-associated endoplasmic reticulum stress in rat osteonecrosis of the femoral head via the Akt/Bad/Bcl-2 signal pathway

et al. 2017

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An excess of glucocorticoids (GCs) is reported to be one of the most common causes of osteonecrosis of the femoral head (ONFH). In addition, GCs can induce bone cell apoptosis through modulating endoplasmic reticulum (ER) stress. Among the three main signal pathways in ER stress, the PERK (protein kinase RNA-like ER kinase)/CHOP (CCAAT-enhancer-binding protein homologous protein) pathway has been considered to be closely associated with apoptosis. Platelet-rich plasma (PRP) has been referred to as a concentration of growth factors and the exosomes derived from PRP (PRP-Exos) have a similar effect to their parent material. The enriched growth factors can be encapsulated into PRP-Exos and activate Akt and Erk pathways to promote angiogenesis. Activation of the Akt pathway may promote the expression of anti-apoptotic proteins like Bcl-2, while CHOP can inhibit B-cell lymphoma 2 (Bcl-2) expression to increase the level of cleaved caspase-3 and lead to cell death. Consequently, we hypothesized that PRP-Exos prevent apoptosis induced by glucocorticoid-associated ER stress in rat ONFH via the Akt/Bad/Bcl-2 signal pathway. To verify this hypothesis, a dexamethasone (DEX)-treated in vitro cell model and methylprednisolone (MPS)-treated in vivo rat model were adopted. Characterization of PRP-Exos, and effects of PRP-Exos on proliferation, apoptosis, angiogenesis, and osteogenesis of cells treated with GCs in vitro and in vivo were examined. Furthermore, the mechanism by which PRP-Exos rescue the GC-induced apoptosis through the Akt/Bad/Bcl-2 pathway was also investigated. The results indicate that PRP-Exos have the capability to prevent GC-induced apoptosis in a rat model of ONFH by promoting Bcl-2 expression via the Akt/Bad/Bcl-2 signal pathway under ER stress.

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Section: Introductionmentioning

confidence: 99%

Exosomes derived from human platelet-rich plasma prevent apoptosis induced by glucocorticoid-associated endoplasmic reticulum stress in rat osteonecrosis of the femoral head via the Akt/Bad/Bcl-2 signal pathway

et al. 2017

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“…However, the cost of MRI scanning is high causing a heavy burden on healthcare system [ [24] , [25] , [26] ], highlighting the need for a convenient laboratory test to detect ONFH development. To our knowledge, urinary miR-150 showed the highest diagnostic power among all known biomarkers for idiopathic ONFH [ 10 , [39] , [40] , [41] , [42] , [43] , [44] ].…”

Section: Discussionmentioning

confidence: 99%

Urinary miRNAs as biomarkers for idiopathic osteonecrosis of femoral head: A multicentre study

Peng

et al. 2021

Journal of Orthopaedic Translation

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“…Primarily, our results showed that traumatic ONFH-Exo could promote cell proliferation, and the rats treated with traumatic ONFH-Exo exhibited increased number of branch points, length of total tube, area of cell migration, and number of invasive cells. Exosomes are a kind of cellsecreted vesicle that are predominantly found in urine, blood, cultured medium, and various biologic fluids, and they serve as promising biomarkers in the diagnosis and treatment of a number of diseases, including pancreatic cancer, chronic kidney disease, and acute coronary syndrome (31). An exosome is internalized upon change of its motion mode to a rapid directed mode (32).…”

Section: Discussionmentioning

confidence: 99%

Down‐regulation of exosomal microRNA‐224‐3p derived from bone marrow–derived mesenchymal stem cells potentiates angiogenesis in traumatic osteonecrosis of the femoral head

Liao

Liu

et al. 2019

FASEB j.

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Traumatic osteonecrosis of the femoral head (ONFH) is a condition leading to the collapse of the femoral head, and the primary treatment is a total hip replacement, which has a poor prognosis. The current study was conducted with the aim of investigating the role of exosomes from bone marrow‐derived mesenchymal stem cells (BM‐MSCs) carrying microRNA‐224‐3p (miR‐224‐3p) in traumatic ONFH. Initially, a microarray analysis was performed to screen the differentially expressed genes and miRs associated with traumatic ONFH. Patients with traumatic and nontraumatic ONFH were enrolled, and HUVECs were obtained. The BM‐MSCs‐derived exosomes were purified and characterized, after which HUVECs were cocultured with exosomes. The functional role of miR‐224‐3p in traumatic ONFH was determined using ectopic expression, depletion, and reporter assay experiments. Endothelial cell proliferation, migration, invasion abilities, and angiogenesis were evaluated. Based on microarray analysis, miR‐224‐3p was found to be down‐regulated, whereas focal adhesion kinase family interacting protein of 200 kDa (FIP200) was up‐regulated in ONFH. Traumatic ONFH exosomes resulted in the up‐regulation of FIP200 and down‐regulation of miR‐224‐3p. FIP200 was confirmed to be a target gene of miR‐224‐3p. Exosomes were internalized by vascular endothelial cells. The down‐regulation of exosomal miR‐224‐3p was observed to promote endothelial cell proliferation, migration, invasion abilities, angiogenesis, and FIP200 expression. In addition, FIP200 overexpression promoted angiogenesis. In summary, the results highly indicated that lower miR‐224‐3p levels in exosomes derived from BM‐MSCs promote angiogenesis of traumatic ONFH by up‐regulating FIP200. The present study provides a potential strategy for the treatment of traumatic ONFH.—Xu, H.‐J., Liao, W., Liu, X.‐Z., Hu, J., Zou, W.‐Z., Ning, Y., Yang, Y., Li, Z.‐H. Down‐regulation of exosomal microRNA‐224‐3p derived from bone marrow‐derived mesenchymal stem cells potentiates angiogenesis in traumatic osteonecrosis of the femoral head. FASEB J. 33, 8055–8068 (2019). http://www.fasebj.org

show abstract

Circulating exosome levels in the diagnosis of steroid-induced osteonecrosis of the femoral head

Cited by 28 publications

References 11 publications

Exosomes derived from human platelet-rich plasma prevent apoptosis induced by glucocorticoid-associated endoplasmic reticulum stress in rat osteonecrosis of the femoral head via the Akt/Bad/Bcl-2 signal pathway

Exosomes derived from human platelet-rich plasma prevent apoptosis induced by glucocorticoid-associated endoplasmic reticulum stress in rat osteonecrosis of the femoral head via the Akt/Bad/Bcl-2 signal pathway

Urinary miRNAs as biomarkers for idiopathic osteonecrosis of femoral head: A multicentre study

Down‐regulation of exosomal microRNA‐224‐3p derived from bone marrow–derived mesenchymal stem cells potentiates angiogenesis in traumatic osteonecrosis of the femoral head

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