Circulating Endothelial Progenitor Cells, C-Reactive Protein and Severity of Coronary Stenosis in Chinese Patients with Coronary Artery Disease

Wang, Hai Ya; Ping, Guichen; Ji, Kai; Shen, Wei Feng; Fan, Chunling; Lü, Lin; Zhu, Dingliang

doi:10.1291/hypres.30.133

Cited by 44 publications

(40 citation statements)

References 16 publications

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“…Our previous study suggested that the number of circulating EPCs was significantly reduced in patients with coronary artery disease compared with control subjects. 16 In this study, we showed that the number of circulating EPCs was lower in patients with cerebrovascular disease 24 hours after acute stroke than in control subjects. Our findings are consistent with previous reports and further support the idea that the number of EPCs is usually decreased in patients with cerebrovascular disease.…”

Section: Discussionmentioning

confidence: 57%

Circulating endothelial progenitor cells in Chinese patients with acute stroke

et al. 2009

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To test the hypothesis that a mobilization of endothelial progenitor cells (EPCs) occurs after acute cerebrovascular diseases, we evaluated the number of EPCs in the process of acute stroke. A total of 203 individuals were examined, including 100 patients with ischemic strokes, 36 patients with hemorrhagic strokes and 67 healthy controls. Ninety-eight patients were observed at days 1, 7, 14 and 28 after acute stroke. Circulating EPCs were defined by the surface markers CD133/KDR and analyzed by flow cytometry. Serum high sensitivity C-reactive protein (hs-CRP) concentrations were determined by particle-enhanced immunonephelometry using the N high sensitivity CRP Reagent. Patients with acute stroke had lower numbers of EPCs (0.037±0.001/100 peripheral blood mononuclear cells (PMNCs) vs. 0.06±0.002/100 PMNCs, Po0.05) and higher levels of serum hs-CRP (1.99 vs. 0.03 mg per 100 ml, Po0.05) than control subjects after adjusting for age, sex, body mass index (BMI) and blood pressure. There were no differences in EPCs counts or serum hs-CRP levels between patients with ischemic and hemorrhagic stroke. In univariate analyses, BMI, age, systolic blood pressure (SBP), diastolic blood pressure, low-density lipoprotein (LDL), total cholesterol (T-cho), blood glucose and hs-CRP (Po0.001) were inversely correlated with EPCs counts. Multivariate analyses showed SBP and total cholesterol as independent predictors of EPCs levels. The number of EPCs gradually increased at day 7 after acute onset, remained elevated at day 14; and returned to baseline by day 28. Our results suggest a possible contribution of circulating EPCs in acute stroke. SBP and total cholesterol are independent factors of reduced EPCs numbers. A transient early increment of EPCs may result from the mobilization of EPCs in response to stroke stress.

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Section: Discussionmentioning

confidence: 57%

Circulating endothelial progenitor cells in Chinese patients with acute stroke

et al. 2009

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“…So far, the investigation of EPC number in relation to hsCRP or hypertension led to heterogeneous results. [14][15][16][18][19][20] However, for determination of extremely rare eventsFas is the case in EPC analysisFa very high number of PBMCs must be analyzed to obtain reliable results. As an aside, we had the possibility to evaluate about 30-50 times more events in this study compared with most of the previous reports.…”

Section: Discussionmentioning

confidence: 99%

“…As an aside, we had the possibility to evaluate about 30-50 times more events in this study compared with most of the previous reports. 15,16,[18][19][20] …”

Section: Discussionmentioning

confidence: 99%

“…18,[24][25][26][27][28][29] In spite of the fact that freshly isolated EPCs (as analyzed by FACS measurement) have little in common with cultured CFUs, 30,31 the latter reflect the tendencies of EPCs in 10 out of 14 studied obesity-related risk factors in the report at hand. Usually, CFUs are counted in several randomly selected so-called 'high-power fields'.…”

Section: Endothelial Progenitor Cells In Obesity K Tobler Et Almentioning

confidence: 99%

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Reduction of both number and proliferative activity of human endothelial progenitor cells in obesity

et al. 2010

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Objective: Circulating endothelial progenitor cells (EPCs), responsible for neoangiogenesis and vascular repair, negatively correlate with vascular dysfunction and atherosclerotic risk factors. Because obesity may have a crucial role in the development of endothelial dysfunction, this study evaluated the number and proliferative activity of circulating human EPCs in obese (body mass index (BMI) ¼ 48±9, n ¼ 45) compared with lean (23±2, n ¼ 45) volunteers. Methods: EPCs were quantified after isolation of peripheral blood mononuclear cells (PBMCs) using fluorescence-activated cell sorting analyses. In addition, plated PBMCs developed colony-forming units (CFUs) from which 'outgrowth' endothelial cells (OECs) sprouted and differentiated into mature endothelial cells. Growth rates were monitored by periodical microscopic evaluation. Cell-cycle protein expression was determined by western blot analyses. Results: BMI negatively correlated (Po0.01) with the number of.500) and CD133 þ /KDR þ (r ¼ À0.282) EPCs. Insulin, leptin, HbA 1c , high-sensitivity C-reactive protein and hypertension, as well as diminished high-density lipoprotein and apolipoprotein A1, were not only associated with obesity but also with significantly reduced EPC levels. Applying selective culture conditions, EPC-CFUs differentiated into OECs that proliferated more slowly when derived from obese compared with lean subjects (obese: 19.9 ± 2.2% vs lean: 30.9 ± 3.2% grown area per week, Po0.01). The reduced proliferation was reflected by decreased (Po0.05, n ¼ 24 for each group) expression of cell-cyclepromoting cyclins and E2F-1, by hypophosphorylation of retinoblastoma protein and by increased (Po0.05, n ¼ 24 for each group) expression of the cell-cycle inhibitor p21 WAFÀ1/Cip1 . Conclusions: Reduced numbers of EPCs along with their premature senescence, as shown in this study, could function as early contributors to the development and progression of vascular dysfunction in obesity.

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“…Vasa et al reported that the circulating EPCs levels were significantly reduced in patients with CAD compared to those without CAD (Vasa et al, 2001). Wang and coworkers indicated that decreased number and activity of EPCs were observed in patients with stable CAD, and EPC levels were negatively correlated with the severity of coronary stenosis assessed by Gensini score (Wang et al, 2007). Fadini et al also reported that EPCs were significantly reduced in subjects with increased intimamedia thickness (Fadini et al, 2006), implying that depletion of EPCs may be an independent predictor of subclinical atherosclerosis.…”

Section: Endothelial Progenitor Cells and Atherosclerosismentioning

confidence: 99%

Endothelial Progenitor Cell in Cardiovascular Diseases

Huang¹

2012

Traditional and Novel Risk Factors in Atherothrombosis

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Circulating Endothelial Progenitor Cells, C-Reactive Protein and Severity of Coronary Stenosis in Chinese Patients with Coronary Artery Disease

Cited by 44 publications

References 16 publications

Circulating endothelial progenitor cells in Chinese patients with acute stroke

Circulating endothelial progenitor cells in Chinese patients with acute stroke

Reduction of both number and proliferative activity of human endothelial progenitor cells in obesity

Endothelial Progenitor Cell in Cardiovascular Diseases

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