Circulating endothelial microparticles and miR-92a in acute myocardial infarction

Abstract

Microparticles (MPs) and miRNAs have been shown to play important roles in coronary artery disease (CAD) by monitoring endothelial dysfunction. The present study aims to investigate the diagnostic value of endothelial MPs (EMPs) and miRNAs (miR-92a or miR-23a) as biomarkers in distinguishing patients with acute myocardial infarction (AMI) from those with CAD. Plasma samples from 37 patients with AMI, 42 patients with stable CAD (SCAD), and 35 healthy adults were collected for investigation in the present study. The numbers of CD31+/CD42b− MPs, CD31+/CD42b+ MPs, and CD31−/CD42b− MPs were measured by flow cytometry and the levels of miR-92a and miR-23a were analyzed using reverse transcription-quantitative PCR. Moreover, cardiac troponin I (cTnI) expression was detected by ELISA to serve as a routine diagnostic parameter. The number of CD31+/CD42b− was higher in AMI group than those in SCAD and healthy groups. Besides, the expression of miR-92a was higher in AMI group compared with two other groups. Furthermore, evidence showed that there was a positive correlation between the levels of CD31+/CD42b− MPs and miR-92a. Finally, the receiver operating characteristic (ROC) curve revealed that the area value under the curve of CD31+/CD42b− MPs, miR-92a and cTnI was 0.893, 0.888, and 0.912 respectively. CD31+/CD42b− MPs and miR-92a might have great potential to provide diagnostic value for AMI and could probably regulate the endothelial dysfunction in AMI patients.

Integration of “Omics” Strategies for Biomarkers Discovery and for the Elucidation of Molecular Mechanisms Underlying Brugada Syndrome

“…miRNA profiling offers great opportunity of investigation, aiming at understanding the molecular mechanisms underlying gene expression in cardiovascular development and disease. The role of miRNAs turns out to be determinant under different conditions, including arrhythmias . Among the miRNAs whose expression is coherently substantiated from previously published data, we found of particular importance miR‐92a, miR‐320b, and miR‐425‐5p.…”

Noncoding RNAs as Biomarkers for Acute Coronary Syndrome

“…The expression of circulating let-7b was decreased in AMI patients [55], but it was significantly upregulated in CD34+ progenitor cells mobilized by STEMI patients [73]. Circulating miR-92a expression was increased in patients with AMI or unstable angina [42,74,75], whereas miR-92a was decreased in peripheral blood monocytes of STEMI patients [16]. Compared with healthy controls, the expression of circulating miR-134-5p in STEMI patients was significantly downregulated [63].…”

Non-Exosomal and Exosomal Circulatory MicroRNAs: Which Are More Valid as Biomarkers?

“…MVs/MPs can be found in blood circulation (Lacroix et al, 2013;Giannella et al, 2017) as well as other body fluids, such as urine (Smalley et al, 2008), as a result of the activation of cell membrane and/or apoptosis processes (Jesel et al, 2013). MVs/ MPs can be shed form various types of cells, such as leukocytes (Pieper et al, 2018), erythrocytes (Yuan et al, 2018), platelets (Diehl et al, 2012;Maugeri et al, 2018), or endothelial cells (Giannella et al, 2017;Zhang et al, 2017). The shed MVs/MPs may or may not contain receptors of their parental cells (Mause et al, 2010;Morel et al, 2011).…”

“…Perhaps, the availability of extensive information regarding exosomes contributes to this phenomenon. Only four studies selected in this review (Diehl et al, 2012;Jansen et al, 2016;Giannella et al, 2017;Zhang et al, 2017) focused solely on MVs/MPs ( Table 2).…”