Circulating Endothelial Cells and Platelet Microparticles in Mitral Valve Disease With and Without Atrial Fibrillation

Idriss, Naglaa K.; Blann, Andrew D.; Sayed, Douaa; Gaber, Marwa A.; Hassen, Hosny A.; Kishk, Yehia Taha

doi:10.1177/0003319714546183

Cited by 9 publications

(9 citation statements)

References 44 publications

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“…However, in accordance with our data, human male patients appear to have higher circulating oxLDL concentrations compared with women . Furthermore, in humans, oxLDL has been determined to be increased in patients with MR if complicated by atrial fibrillation compared with clinically healthy controls …”

Section: Discussionsupporting

confidence: 90%

Markers of Oxidative Stress in Dogs with Myxomatous Mitral Valve Disease are Influenced by Sex, Neuter Status, and Serum Cholesterol Concentration

Reimann

Häggström

Møller

et al. 2017

Veterinary Internal Medicne

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Background: Cardiovascular disease has been associated with oxidative stress, which has been suggested to contribute to myocardial remodeling in human patients. Little is known about the relationship between myxomatous mitral valve disease (MMVD) and oxidative stress in dogs.Objective: To determine whether clinical stage of MMVD is associated with changes in the plasma concentrations of certain markers of oxidative stress in clinically healthy dogs and dogs with MMVD.Animals: Seventy five privately owned dogs: 59 cavalier King Charles Spaniels (CKCS) with different severities of MMVD and 16 dogs of different breeds with clinical signs of congestive heart failure (CHF) caused by MMVD.Methods: Markers of oxidative stress including malondialdehyde (MDA), oxidized low-density lipoprotein (oxLDL), and vitamin E (a-tocopherol and c-tocopherol) were measured in plasma and their association with clinical stage of MMVD was assessed by regression analyses.Results: Plasma oxLDL concentration was significantly lower in female dogs compared with males (P = .01). Significantly higher plasma c-tocopherol concentrations were found in neutered (P = .003) dogs. Vitamin E (a-tocopherol [P = .0004] and c-tocopherol [P = .003]) was associated with body condition score (BCS), but the association disappeared when cholesterol was included in the analyses. All markers of oxidative stress (MDA, oxLDL, and vitamin E) were positively associated with serum cholesterol concentration (P ≤ .04), but none were associated with clinical stage of MMVD.Conclusions: In conclusion, markers of oxidative stress are associated with sex, BCS, neuter status, and cholesterol. The results cannot confirm a relationship between oxidative stress and clinical stage of the disease in dogs with MMVD.

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Section: Discussionsupporting

confidence: 90%

Markers of Oxidative Stress in Dogs with Myxomatous Mitral Valve Disease are Influenced by Sex, Neuter Status, and Serum Cholesterol Concentration

Reimann

Häggström

Møller

et al. 2017

Veterinary Internal Medicne

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“…In the Framingham Heart study circulating endothelium-derived microparticles were associated with cardiometabolic risk factors [39]. Furthermore, either elevated concentration or altered composition of this EV subtype could be found in a broad variety of diseases like vasculitis [40], acute coronary syndrom [41], mitral valve disease [42] or thrombotic thrombocytopenic purpura [43]. The relatively high EEV levels in BM of healthy donors might reflect the fact that endothelium is highly abundant and active in BM.…”

Section: Discussionmentioning

confidence: 99%

Human bone marrow contains high levels of extracellular vesicles with a tissue-specific subtype distribution

et al. 2018

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IntroductionExtracellular vesicles (EV) are shed from a broad variety of cells and play an important role in activation of coagulation, cell to cell interaction and transport of membrane components. They are usually measured as circulating EV in peripheral blood (PB) and other body fluids. However, little is known about the distribution, presence and impact of EV and their subpopulations in bone marrow (BM). In our study, we focused on the analysis of different EV subtypes in human BM as compared to EV subsets in PB.MethodsEV in BM and PB from 12 healthy stem cell donors were measured by flow-cytometry using Annexin V and cell-specific antibodies for hematopoietic stem cells, leucocytes, platelets, red blood cells, and endothelial cells. Additionally, concentrations of tissue factor-bearing EV were evaluated.ResultsHigh numbers of total EV were present in BM (median value [25–75 percentile]: 14.8 x109/l [8.5–19.3]). Non-significantly lower numbers of total EV were measured in PB (9.2 x109/l [3.8–14.5]). However, distribuation of EV subtypes showed substantial differences between BM and PB: In PB, distribution of EV fractions was similar as previously described. Most EV originated from platelets (93.9%), and only few EV were derived from leucocytes (4.5%), erythrocytes (1.8%), endothelial cells (1.0%), and hematopoietic stem cells (0.7%). In contrast, major fractions of BM-EV were derived from red blood cells or erythropoietic cells (43.2%), followed by megacaryocytes / platelets (27.6%), and by leucocytes as well as their progenitor cells (25,7%); only low EV proportions originated from endothelial cells and hematopoietic stem cells (2.0% and 1.5%, respectively). Similar fractions of tissue factor—bearing EV were found in BM and PB (1.3% and 0.9%).ConculsionTaken together, we describe EV numbers and their subtype distribution in the BM compartment for the first time. The tissue specific EV distribution reflects BM cell composition and favours the idea of a BM–PB barrier existing not only for cells, but also for EV.

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“…To date, several case-control studies have reported increased oxLDL in the general CKD population [9,20] , as well as in AF patients [21] , and recently, oxLDL has been linked to the risk of developing cardiovascular disease in AF patients without associated comorbidities [22] . Our findings extend those observations to the interplay of AF, OS and renal dysfunction.…”

Section: Discussionmentioning

confidence: 99%

Oxidized Low-Density Lipoprotein Predicts the Development of Renal Dysfunction in Atrial Fibrillation

et al. 2016

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Background/Aim: To investigate the role of oxidative stress (OS) in the development of chronic kidney disease (CKD) in atrial fibrillation (AF). Methods: We compared OS burden, determined at study inclusion as plasma concentrations of oxidized low-density lipoprotein (oxLDL), between stable AF patients (n = 256, mean age: 62.8 ± 9.3 years; 60.9% males) with preserved renal function, defined as an estimated glomerular filtration rate (eGFR) ≥ 60 ml/ min/1.73 m 2 , and a matched control group in sinus rhythm (n = 138, mean age: 61.5 ± 11.2 years; 60.9% males). During the prospective follow-up of AF patients, we investigated the association and prognostic validity of oxLDL for CKD development, diagnosed as a sustained decline in eGFR to <60 ml/min/1.73 m 2 . Results: AF patients had a higher mean oxLDL (76.2 ± 21.7 U/l) compared to sinus rhythm controls (61.6 ± 13.1 U/l; p < 0.001). AF presence independently predicted increased oxLDL levels in the study cohort [β = 14.7; 95% confidence interval (CI), 10.7-18.7; p < 0.001]. Over a median 4-year follow-up, 19.9% of AF patients developed CKD. Adjusting for all clinical covariates, oxLDL (per tertile) was associated with a hazard ratio of 2.17 for CKD occurrence (95% CI, 1.40-3.35; p < 0.001). AF patients in the upper oxLDL tertile ( ≥ 88.7 U/l) had a 3.70-fold (95% CI, 1.55-8.81) higher risk for CKD compared to the lower oxLDL tertile (<67.0 U/l) patients (p < 0.001). oxLDL improved discriminative validity ( cstatistic increment: 0.041, 95% CI, 0.007-0.075, p = 0.017), and increased the net reclassifica- tion and integrated discrimination for CKD risk by 12.4 and 6.0%, respectively (both p < 0.001). Conclusions: oxLDL is increased in AF patients compared to sinus rhythm controls. oxLDL has an independent association and an incremental predictive value that might complement clinical CKD risk assessment in AF patients following further research.

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Circulating Endothelial Cells and Platelet Microparticles in Mitral Valve Disease With and Without Atrial Fibrillation

Cited by 9 publications

References 44 publications

Markers of Oxidative Stress in Dogs with Myxomatous Mitral Valve Disease are Influenced by Sex, Neuter Status, and Serum Cholesterol Concentration

Markers of Oxidative Stress in Dogs with Myxomatous Mitral Valve Disease are Influenced by Sex, Neuter Status, and Serum Cholesterol Concentration

Human bone marrow contains high levels of extracellular vesicles with a tissue-specific subtype distribution

Oxidized Low-Density Lipoprotein Predicts the Development of Renal Dysfunction in Atrial Fibrillation

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