Circulating Cell-Free DNA in Physical Activities

Vittori, Leydi Natalia; Tarozzi, Andrea; Latessa, Pasqualino Maietta

doi:10.1007/978-1-4939-8973-7_14

Cited by 25 publications

(17 citation statements)

References 42 publications

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“…Exercise resulted in the surge of plasma cfDNA independently of workout associated with various sport disciplines and types of training such as weightlifting 4 , running 5 , soccer 6 , cycling 7 , rowing 8 , strength training 9 or repeated sprint 6 . Concentration of post-exercise cfDNA positively correlated with the duration and the intensity of aerobic running 10 as well as with selected markers of muscle damage 11 . Moreover, the ratio of post- to pre-exercise cfDNA associated with the distance covered by soccer players during the game 6 .…”

Section: Introductionmentioning

confidence: 95%

“…In another study, increased cfDNA in response to heavy resistance exercise was described to have possible predictive value of muscle-performance decrease within 2 days after the bout 12 . Therefore, measurements of exercise-related variations of cfDNA can be used for monitoring of training load or diagnosis and prevention of overtraining syndrome 11 .…”

Section: Introductionmentioning

confidence: 99%

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Decreased integrity of exercise-induced plasma cell free nuclear DNA – negative association with the increased oxidants production by circulating phagocytes

Stawski

Walczak

Perdas

et al. 2019

Sci Rep

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Strenuous exercise increases circulating cell free DNA (cfDNA) and stimulates blood phagocytes to generate reactive oxygen species (ROS) which may induce DNA strand breaks. We tested whether: (A) elevated cfDNA in response to three repeated bouts of exhaustive exercise has decreased integrity; (B) each bout of exercise increases luminol enhanced whole blood chemiluminescence (LBCL) as a measure of ROS production by polymorphonuclear leukocytes. Eleven men performed three treadmill exercise tests to exhaustion separated by 72 hours of resting. Pre- and post-exercise concentrations and integrity of cf nuclear and mitochondrial DNA (cf n-DNA, cf mt-DNA) and resting (r) and fMLP (n-formyl-methionyl-leucyl-phenylalanine)-stimulated LBCL were determined. Each bout increased concentrations of cf n-DNA by more than 10-times which was accompanied by about 2-times elevated post-exercise rLBCL and fMLP-LBCL. Post-exercise cf n-DNA integrity (integrity index, I229/97) decreased after the first (0.59 ± 0.19 vs. 0.48 ± 0.18) and second (0.53 ± 0.14 vs. 0.44 ± 0.17) bout of exercise. There were negative correlations between I229/97 and rLBCL (ƍ = –0.37), and I229/97 and fMLP-LBCL (ƍ = –0.40) – analysis of pooled pre- and post-exercise data (n = 66). cf mt- DNA integrity (I218/78) did not alter in response to exercise. This suggests an involvement of phagocyte ROS in cf n-DNA strand breaks in response to exhaustive exercise.

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Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Decreased integrity of exercise-induced plasma cell free nuclear DNA – negative association with the increased oxidants production by circulating phagocytes

Stawski

Walczak

Perdas

et al. 2019

Sci Rep

View full text Add to dashboard Cite

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“…Altered profiles of CFNAs have been detected under physiological conditions, e.g. by making sport, suboptimal conditions such as overtraining syndrome in physical exercises [ 3 ], acute and chronic pathological conditions including sepsis, stroke, trauma, myocardial infarction, autoimmune diseases and cancers [ 4 ]. To this end, certainly the area of oncological research is particularly advanced implementing ctDNA and miRNA detection and quantification for diagnostic and treatment purposes [ 5 ].…”

Section: Liquid Biopsy Is Instrumental For Predictive Diagnostics Andmentioning

confidence: 99%

“…Consequently, the question is—how to distinguish between beneficial physical activity on one hand and damaging exercise-induced oxidative stress on the other hand, when providing recommendations at individual level? Circulating CFNAs might be helpful answering this question, since their patterns strongly depend on the intensity and duration of exercise being complementary to specific metabolic markers such as lactate and creatine kinase recognising muscle damage [ 3 , 65 ]. To this end, the overtraining and induced inflammation are well reflected in C-reactive protein (CRP) levels as the marker of inflammation and highly increased concentration of plasma cfDNA in proportion to training load [ 66 ].…”

Section: Physical Activity and Exercise-induced Oxidative Stressmentioning

confidence: 99%

Cell-free nucleic acid patterns in disease prediction and monitoring—hype or hope?

et al. 2020

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Interest in the use of cell-free nucleic acids (CFNAs) as clinical non-invasive biomarker panels for prediction and prevention of multiple diseases has greatly increased over the last decade. Indeed, circulating CFNAs are attributable to many physiological and pathological processes such as imbalanced stress conditions, physical activities, extensive apoptosis of different origin, systemic hypoxic-ischemic events and tumour progression, amongst others. This article highlights the involvement of circulating CFNAs in local and systemic processes dealing with the question, whether specific patterns of CFNAs in blood, their detection, quantity and quality (such as their methylation status) might be instrumental to predict a disease development/progression and could be further utilised for accompanying diagnostics, targeted prevention, creation of individualised therapy algorithms, therapy monitoring and prognosis. Presented considerations conform with principles of 3P medicine and serve for improving individual outcomes and cost efficacy of medical services provided to the population.

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“…Дальнейшее изменение соотношения белков внеклеточного матрикса -эластина и коллагена приводит к изменению механических свойств стенки сосуда, что запускает ряд каскадных механизмов воспалительного, протеазного и оксидативного сдвига и замыкает порочный круг. Последующие изменения приводят к развитию различных патологий, в том числе атеросклероза [41,42].…”

Section: обзоры литературыunclassified

The role of physical activity in the development of atherosclerotic lesions of the vascular wall

Markin¹,

Markina²,

Sukhorukov³

et al. 2019

Clin. Exp. Morphology

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1 ФГБНУ Научно-исследовательский институт морфологии человека, Москва, Россия 2 ФГБУ Национальный медицинский исследовательский центр кардиологии Минздрава России, Москва, Россия 3 ФГБНУ Научно-исследовательский институт общей патологии и патофизиологии, Москва, Россия 4 Институт исследований атеросклероза, Сколковский инновационный центр, Москва, Россия В обзоре рассматриваются основные механизмы повреждающего действия чрезмерных физических нагрузок на клеточные структуры эндотелия сосудов и развития связанных с этим процессом системных патологических состояний. Общепринятое мнение о пользе физической активности для профилактики сердечно-сосудистых заболеваний не подвергается сомнению. Тем не менее сегодня широкое распространение получили различные тренировочные программы с дискуссионным соотношением пользы и вреда. Отличительными чертами такого типа физической активности являются высокая степень интенсивности физической нагрузки и большие тренировочные объемы. Нарушение режимов тренировки и отдыха при таком подходе к тренировкам часто приводит к развитию синдрома перетренированности. Понимание причин развития данного состояния на молекулярном и клеточном уровне позволит повысить эффективность планирования тренировочного процесса у соревнующихся атлетов и продлить спортивное долголетие пожилым спортсменам и любителям спорта. Ключевые слова: атеросклероз, воспаление, чрезмерные физические нагрузки, артериальная ригидность. Для корреспонденции: Александр Михайлович Маркин. Финансирование. Исследование выполнено при поддержке Российского научного фонда (грант № 19-15-00010). Конфликт интересов. Авторы заявляют об отсутствии конфликта интересов.The main mechanisms of the damaging effect of excessive physical exertion on the cellular structures of the vascular endothelium and the development of systemic pathological conditions associated with this process are covered in this paper. The generally accepted view on the benefits of physical activity for the prevention of cardiovascular disease is not in doubt. However, today various training programs of debatable benefits and risks ratio are widely used. High intensity high volume training is the distinctive feature of this type of physical activity. Violation of the training and rest schedules in this approach often leads to the overtraining syndrome. Understanding the causes of this condition at the molecular and cellular levels may increase the efficiency of training planning for competing athletes and prolong sport participation of the elderly athletes and sports enthusiasts.

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Circulating Cell-Free DNA in Physical Activities

Cited by 25 publications

References 42 publications

Decreased integrity of exercise-induced plasma cell free nuclear DNA – negative association with the increased oxidants production by circulating phagocytes

Decreased integrity of exercise-induced plasma cell free nuclear DNA – negative association with the increased oxidants production by circulating phagocytes

Cell-free nucleic acid patterns in disease prediction and monitoring—hype or hope?

The role of physical activity in the development of atherosclerotic lesions of the vascular wall

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