Circulating cell-free DNA in patients with alveolar echinococcosis

Baraquin, Alice; Hervouet, Éric; Richou, Carine; Flori, Pierre; Peixoto, Paul; Azizi, Amel; Delabrousse, É; Blagosklonov, Oleg; Umhang, Gérald; Bresson‐Hadni, Solange; Valot, Benoı̂t; Grenouillet, Frédéric

doi:10.1016/j.molbiopara.2018.04.004

Cited by 23 publications

(24 citation statements)

References 43 publications

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“…DNA in the AE sample could be due to the mixture of necrotic parasite tissue and actively proliferating tissues. This is in line with the previous finding that the sensitivity of PCR-based methods in AE samples was higher than in CE samples [31][32][33]. As we only collected one AE sample, it needed further verification with more samples.…”

Section: Plos Neglected Tropical Diseasessupporting

confidence: 91%

“…DNA in plasma is extremely low, whose proportion ranged 1.8e-5 to 4.0e-9 in these samples (Fig 2). Indeed, this low concentration may explain the low sensitivity of the PCR-based methods [31][32][33]. Besides the low concentration, we identified the difference between the cell-free Echinococcus spp.…”

Section: Plos Neglected Tropical Diseasesmentioning

confidence: 92%

“…Several attempts were made using cfDNA in Echinococcus spp. detection from plasma or serum with PCR-based methods, but their overall sensitivity was only 20% to 25% [31][32][33]. The low sensitivity could be due to non-existence, or low concentration of cfDNA of the parasite in the circulation, which showed our limited understanding of the cfDNA of Echinococcus spp.…”

Section: Plos Neglected Tropical Diseasesmentioning

confidence: 99%

“…The existence of E. granulosus DNA in the blood circulation of the echinococcosis patients was questioned by Chaya et al who believed that the cfDNA of the parasite would only enter the blood circulation when the hydatid cyst(s) ruptured [31]. Baraquin et al confirmed the existence of cfDNA of E. multilocularis in AE patients and used the very low concentration of cfDNA to explain the low sensitivity of their PCR test [32]. Low concentration of target DNA in plasma is a common situation for cfDNA studies.…”

Section: Plos Neglected Tropical Diseasesmentioning

confidence: 99%

“…Cell-free Echinococcus spp. DNA had already been suggested as a biomarker for echinococcosis [21], and its existence in plasma or serum was proven with PCR-based methods [31][32][33], though with rather low sensitivities (20-25%) [31][32][33] Low sensitivity prevents further application of using plasma cfDNA in the diagnosis of echinococcosis. The unsatisfactory performance of the previous attempts could be due to three possible reasons.…”

Section: Introductionmentioning

confidence: 99%

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Comprehensive characterization of plasma cell-free Echinococcus spp. DNA in echinococcosis patients using ultra-high-throughput sequencing

et al. 2020

PLoS Negl Trop Dis

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Background Echinococcosis is a life-threatening parasitic disease caused by Echinococcus spp. tapeworms with over one million people affected globally at any time. The Echinococcus spp. tapeworms in the human body release DNA to the circulatory system, which can be a biomarker for echinococcosis. Cell-free DNA (cfDNA) is widely used in medical research and has been applied in various clinical settings. As for echinococcosis, several PCR-based tests had been trialed to detect cell-free Echinococcus spp. DNA in plasma or serum, but the sensitivity was about 20% to 25%. Low sensitivity of PCR-based methods might be related to our limited understanding of the features of cell-free Echinococcus spp. DNA in plasma, including its concentration, fragment pattern and release source. In this study, we applied ultra-high-throughput sequencing to comprehensively investigate the characteristics of cell-free Echinococcus spp. DNA in plasma of echinococcosis patients. Methodology/Principal findings We collected plasma samples from 23 echinococcosis patients. Total plasma cfDNA was extracted and sequenced with a high-throughput sequencing platform. An average of 282 million read pairs were obtained for each plasma sample. Sequencing data were analyzed PLOS NEGLECTED TROPICAL DISEASES

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Section: Plos Neglected Tropical Diseasessupporting

confidence: 91%

Section: Plos Neglected Tropical Diseasesmentioning

confidence: 92%

Section: Plos Neglected Tropical Diseasesmentioning

confidence: 99%

Section: Plos Neglected Tropical Diseasesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Comprehensive characterization of plasma cell-free Echinococcus spp. DNA in echinococcosis patients using ultra-high-throughput sequencing

et al. 2020

PLoS Negl Trop Dis

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The role of cell‐free DNA in non‐invasive diagnosis of urinary tract infection

Zhao,

Zhang,

et al. 2024

UroPrecision

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Urinary tract infection (UTI) constitutes a pervasive health concern. UTIs are dichotomously classified into upper and lower strata, and further categorized as either complicated or uncomplicated. Upper UTIs predominantly afflict the renal and ureteral domains, typified by conditions exemplified in pyelonephritis, whereas lower UTIs manifest within the confines of the urethra and bladder. The conventional diagnosis of UTIs relies upon clinical manifestations and urine culture. Common symptoms encompass dysuria, frequent micturition, hematuria, and suprapubic discomfort. Urine culture serves to identify the specific pathogens instigating the infection and assess their antibiotic susceptibility. However, this procedural protocol generally necessitates an approximate duration of 24 h, coupled with an additional 24‐h interval for antibiotic susceptibility testing. In contradistinction, the detection of cell‐free DNA (cfDNA) in the circulatory system presents a potential avenue for non‐invasive diagnostic modalities. Notwithstanding, cfDNA emanates from deteriorating cells, affording insights into the extant cellular demise within the organism. Extensive scholarly inquiry has established a positive correlation between cfDNA concentration in the bloodstream and the incidence of cell death in conditions, such as severe traumas, sepsis, aseptic inflammation, myocardial infarction, and stroke. However, limited investigative effort has been devoted to elucidating the diagnostic potential of cfDNA in the context of UTIs. Consequently, the concentration and constitution of plasma cfDNA harbor substantial promise for non‐invasively diagnosing UTIs. In summation, the utilization of cfDNA in UTI diagnosis remains an incipient area of scholarly pursuit, underscoring the imperative for further research to elucidate the prospective role of plasma cfDNA in non‐intrusively discerning UTIs.

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