Circulating CD14⁺HLA‐DR^−/low Myeloid‐Derived Suppressor Cells as Potential Biomarkers for the Identification of Psoriasis TCM Blood‐Heat Syndrome and Blood‐Stasis Syndrome

Abstract

Psoriasis is a chronic autoimmune disease. Identification of the biomarkers responsible for Traditional Chinese Medicine (TCM) syndromes of psoriasis can help researchers recognize the different aspects of psoriasis and find novel therapeutic targets for the treatment of psoriasis. The current study investigated the levels of circulating Mo-MDSCs and Mo-MDSC-associated immune factors in the peripheral blood of psoriasis patients with different TCM syndromes. We found that the frequency of Mo-MDSCs (CD14+HLA-DR−/low cells) among CD14+ cells from plaque psoriasis patients with blood-stasis (BS) syndrome was significantly increased when compared with healthy controls p<0.001 and blood-heat (BH) syndrome group p<0.001, respectively. However, serum IL-2, IL-4, IL-6, IL-10, IL-17A, TNF-α, IFN-γ, iNOS, Arg-1, and NO concentration showed no statistically significant difference between healthy controls and psoriasis patients as well as no significant difference between the BH and BS syndrome groups. Compared with healthy controls, the mRNA expression of Arg-1, TNF-α, ROR-γ, and PD-L1 was increased, while the mRNA expression of PD-1 and IL-10 was decreased in PBMCs from psoriasis patients. Moreover, the mRNA expression of TNF-α and FOXP3 in PBMCs showed a pronounced statistical difference between the psoriatic BH syndrome group and the BS syndrome group. Therefore, we provide evidence that the percentage of CD14+HLA-DR−/low MDSC/ CD14+ cells and TNF-α and Foxp3 mRNA expression levels in PBMCs are potential biomarkers for distinguishing TCM BH syndrome and BS syndrome.

Chinese Medicine Formula PSORI-CM02 Alleviates Psoriatic Dermatitis via M-MDSCs and Th17 Crosstalk

“…In recent years, CD14 + HLA-DR-/low M-MDSCs have been reported to suppress T-cell activation ( Lauret Marie Joseph et al, 2020 ). In psoriatic patient PBMC, M-MDSCs was increased, compared to the healthy control ( Soler et al, 2016 ; Sun et al, 2020 ). In vivo and in vitro assays demonstrated that Treg cells induced by M-MDSCs in the PBMC of a psoriasis patient presented suppressive functionality reduction.…”

Icariin improves cardiac function and remodeling via the TGF-β1/Smad signaling pathway in rats following myocardial infarction

“…Icariin has a therapeutic effect on coronary heart disease, but its mechanism remains largely unclear. According to TCM principles, ventricular dysfunction and heart failure arise from Qi deficiency and blood stasis [ 36 , 37 ]. In this study, icariin therapy exhibited notable improvements in LVEF and FS while concurrently reducing LVEDD and LVESD compared to the MI group.…”

Emerging Treatment Regimens in Psoriasis: Are There Advantages Over Current Biologic Therapies?

“…69,70 As advances are made in the understanding of mRNA regulation in psoriasis, opportunities will arise for more accurate monitoring and efficacious intervention. 69,71,72 Exclusive discussion of pharmacology creates an incomplete picture of the advances made in psoriasis management. Understanding and combating poor medication adherence is essential to improving clinical outcomes.…”