Circulating antibodies to conjugated tryptophan derivatives of the IDO pathway in amyotrophic lateral sclerosis, Alzheimer's, Parkinson's and multiple sclerosis patients

Duleu, S.; Mangas, A.; Gannes, F. Poulletier de; Tranchant, Martine; Geffard, M.

doi:10.1016/j.immbio.2007.11.001

Cited by 4 publications

(7 citation statements)

References 38 publications

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“…The presence of circulating antibodies directed against conjugated tryptophan metabolites indirectly revealed the overproduction of metabolites associated with hyperactivation of the IDO-1 in AD, as previously described in [23]. However, no previous study had shown the presence of circulating antibodies against THO-pathway-derived metabolites.…”

Section: Resultssupporting

confidence: 54%

“…Bovine serum albumin (BSA) (ID Bio) was also dissolved in 3 mL 2-morpholino-ethanesulfonic acid monohydrate (MES) buffer 10 −1 M (pH 6.3) (Acros). Then, the tryptophan derivatives were mixed with the BSA solution and supplemented with 15 mg N-hydroxysuccinimide (Sigma) and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (Acros) as coupling agents [ 23 ]. The conjugates were synthesized by linking 10 mg kynurenine (Kyn) (Sigma), or 3-hydroxykynurenine (3-OHKyn) (Sigma), kynurenic acid (Kyna) (Acros), Quina (Acros), quinaldic acid (Quinald) (Acros), 3-hydroxyanthranilic acid (3-OHAnthra) (Aldrich), anthranilic acid (Anthra) (Acros), xanthurenic acid (Xantha) (Acros), picolinic acid (Pico) (Acros), or 5-hydroxyindole acetic acid (5-HIAA) (Sigma), to 20 mg BSA.…”

Section: Methodsmentioning

confidence: 99%

“…The molecular coupling ratio of each conjugate was determined by measuring the concentration of tryptophan derivative and BSA at 310–330 nm and 280 nm, respectively, as previously described in [ 23 ], taking into account the molar extinction coefficients after coupling.…”

Section: Methodsmentioning

confidence: 99%

“…ELISA was used to determine the titers of G, M, or A immunoglobulins (Ig). The protocol has been extensively described elsewhere [ 23 – 25 ]. Briefly, polystyrene 96-well plates (NUNC) were coated with 200 μ L solution containing 10–50 μ g/mL tryptophan-derivative conjugates in 0.05 M carbonate buffer (pH 9.6).…”

Section: Methodsmentioning

confidence: 99%

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Circulating Antibodies to IDO/THO Pathway Metabolites in Alzheimer's Disease

Duleu¹,

Mangas

Sevin³

et al. 2010

International Journal of Alzheimer's Disease

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In Alzheimer's disease, indoleamine 2,3-dioxygenase and tryptophan hydroxylase are known to induce an overproduction of neurotoxic compounds, such as quinolinic acid and 3-hydroxykynurenine from the former, and 5-hydroxytryptophol and 5-methoxytryptophol from the latter. Other compounds, such as kynurenic acid, serotonin, and melatonin are produced via the same pathways. An improved ELISA method identified circulating antibodies directed against these compounds, linked to proteins, as previously described for other chronic diseases. This describes how only the A isotype of circulating immunoglobulins recognized a pattern of conjugated tryptophan metabolites in the sera of Alzheimer patients. These data indirectly confirmed the involvement of tryptophan derivatives in the pathogenic processes of Alzheimer's disease. Further studies are required to evaluate the relevance of these antibody patterns in monitoring this disease.

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Section: Resultssupporting

confidence: 54%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Circulating Antibodies to IDO/THO Pathway Metabolites in Alzheimer's Disease

Duleu¹,

Mangas

Sevin³

et al. 2010

International Journal of Alzheimer's Disease

Self Cite

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“…Finally, as of the date of this publication, there are no biomarkers, antibodies or otherwise, that have been demonstrated to confirm the trigger, identify downstream processes that drive progression, or plan successful treatment for patients with sporadic ALS. The proponents of Endotherapia have published papers (13,14) and an abstract (15) on components of their ALS immunoblots, but in our opinion these failed to demonstrate their utility as ALS biomarkers of any kind. ALSUntangled assigns a TOE 'Mechanism' grade of D based on this information (Table II).…”

Section: Mechanism(s)mentioning

confidence: 91%

ALS Untangled No.33 Endotherapia

2016

Amyotrophic Lateral Sclerosis and Frontotemporal Degeneration

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Impact of Aromatic Ring Count on the Ability to Participate in Attractive Interactions. Crystal Structure (X-ray) and Solid State Computational (DFT/QTAIM/RDS/Hirshfeld-surfaces) Study of 1,4-di(2-phenyl-1H-imidazol-4-yl)benzene. A Potential Nanotechnology Intrinsic Component

Latosińska

Maurin

et al. 2015

Crystal Growth & Design

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The crystal structure of 1,4-di(2-phenyl-1H-imidazol-4-yl)benzene (DPI4B) is determined by X-ray diffraction with a final R-factor of 6.42% at room temperature. It crystallizes with a space group P2 1 /c, Z = 4, a = 5.01790(10), b = 28.8971(6), and c = 12.9362(3) Å; β = 98.757(2)°. Two 4-phenyl-1H-imidazole (4PI) fragments are related by inversion symmetry and each makes a dihedral angle of 0°with the benzene rings. Despite the presence of a total number of five homo-and heterocyclic rings in the DPI4B molecule, similarly to 2-phenyl-1H-imidazole (2PI) but in contrast to 1,4-di(1H-imidazol-4-yl)benzene (DI4B) and 4-phenyl-1H-imidazole (4PI), it is almost planar. Each plane containing a terminal phenyl ring makes a small dihedral angle of about 3°with the central benzene ring plane. The average twisting angle of the whole molecule is equal to 7°. The ordering of compounds studied according to the decreasing twisting angle is 4PI > DI4B > DPI4B > 2PI and is the same as that according to hydrogen bond length/strength. In DPI4B bereft of N−H···N bonds, four weak C−H···N bonds of C···N = 3.362 Å and ∠(C, H, N) = 158°; C···N = 3.437 Å and ∠(C, H, N) = 123°; C···N = 3.392 Å and ∠(C, H, N) = 128°and C···N = 3.914 Å and ∠(C, H, N) = 156°, force crystalline packing with almost perpendicular adjacent molecules. Strong π···π stacking allows dense packing of planar array of molecules, with a small interlayer distance of 3.413 Å. The analysis of intermolecular interactions pattern supported by quantum theory of atoms in molecules (QTAIM), reduced density gradient (RDS), and Hirshfeld surfaces approach reveals subtle and diverse types of interactions difficult to describe within standard techniques. QTAIM/Hirshfeld clearly indicate the role of interplay between C−H···N hydrogen bonds, N···π and π···π stacking, and a shift from strong (N−H···N) to very weak (C−H···N) attractive interactions supplemented by π···π displaced pure type stacking upon aromatic ring count. QTAIM/RDS reveal mixed attractive/repulsion interactions forcing flatness of the molecule. Electrostatic potential indicates the complementarity of the regions bonded through the C−H···N in the neighboring molecules, which permits easy overcome of any repulsive interactions in DPI4B that may destroy the flatness of the molecule.

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Circulating antibodies to conjugated tryptophan derivatives of the IDO pathway in amyotrophic lateral sclerosis, Alzheimer's, Parkinson's and multiple sclerosis patients

Cited by 4 publications

References 38 publications

Circulating Antibodies to IDO/THO Pathway Metabolites in Alzheimer's Disease

Circulating Antibodies to IDO/THO Pathway Metabolites in Alzheimer's Disease

ALS Untangled No.33 Endotherapia

Impact of Aromatic Ring Count on the Ability to Participate in Attractive Interactions. Crystal Structure (X-ray) and Solid State Computational (DFT/QTAIM/RDS/Hirshfeld-surfaces) Study of 1,4-di(2-phenyl-1H-imidazol-4-yl)benzene. A Potential Nanotechnology Intrinsic Component

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