Circular RNAs play an important role in late-stage gastric cancer: Circular RNA expression profiles and bioinformatics analyses

Fang, Yantian; Ma, Minzhe; Wang, Jiangli; Liu, Xiaowen; Wang, Yanong

doi:10.1177/1010428317705850

Cited by 19 publications

(10 citation statements)

References 47 publications

(61 reference statements)

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“…23 Also, hsa_circ_0058246 was found to be upregulated in gastric cancer (GC) and associated with tumor recurrence. 24 In addition, increasing studies have shown that circRNAs may serve as important regulators in various cancers. [25][26][27] However, the novel circRNAs and the function they played were still needed to explore.…”

Section: Discussionmentioning

confidence: 99%

<p>Identification of circRNA-miRNA-mRNA Networks for Exploring the Fundamental Mechanism in Lung Adenocarcinoma</p>

Liang¹,

Zhang²,

Zhang³

et al. 2020

OTT

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Background: Circular RNAs (circRNAs) were elucidated to act as competing endogenous RNAs (ceRNAs) and to play significant roles in cancer initiation and progression. We aimed to identify the important circRNAs in lung adenocarcinoma and intended to predict the functions of them via the bioinformatics analysis. Methods: We extracted data from three Gene Expression Omnibus (GEO) datasets, GSE101586, GSE101684, and GSE104854, and identified the common differentially expressed circRNAs. Agarose gel electrophoresis and Sanger sequencing were used to verify these significant circRNAs. Then, qRT-PCR was performed to validate the expression through matched tissues and cell lines. Afterwards, a ceRNA network was constructed and functional analysis was performed to predict the potential mechanisms of circRNAs. Results: Five circRNAs (hsa_circ_0072088, hsa_circ_0082564, hsa_circ_0008274, hsa_-circ_0000519 and hsa_circ_0003528) were identified differentially expressed in the three datasets. Following the Agarose gel electrophoresis and qRT-PCR validation, hsa_circ_0072088 and hsa_-circ_0008274 were chosen for further analysis. A ceRNA network of hsa_circ_0072088 and hsa_circ_0008274 was built based on the CSCD/TargetScan/MiRTarbase/StarBase 3.0. Then, the functional analysis showed that several meaningful terms were identified, such as "epithelial to mesenchymal transition (EMT)", "Cellular response to TGF-β stimulus", "MAPK signaling pathway", and "PI3K-AKT signaling pathway". Finally, several significant circRNA/miRNA/mRNA regulatory axes, which were predicted relating to cancer progress, were noted from the network. Conclusion: We identified significant circRNAs and meaningful circRNA-miRNA-mRNA networks to provide novel insight into pathogenesis and therapy of lung adenocarcinoma.

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Section: Discussionmentioning

confidence: 99%

<p>Identification of circRNA-miRNA-mRNA Networks for Exploring the Fundamental Mechanism in Lung Adenocarcinoma</p>

Liang¹,

Zhang²,

Zhang³

et al. 2020

OTT

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“…Recently, several studies showed that circRNAs could be utilized as potential biomarkers for the prediction of tumor recurrence. For example, the expression of hsa_ circ_0058246 was statistically significantly increased in patients who suffered from the recurrence of gastric cancer (GC) [ 41 ]. In another study, patients with lower levels of circPVT1 in GC had a significantly shorter progression-free survival (PFS) than those with high levels of circPVT1, indicating that circPVT1 might be a biomarker for tumor recurrence [ 42 ].…”

Section: Circrnas As Potential Biomarkers In Cancermentioning

confidence: 99%

Circular RNAs: a new frontier for cancer diagnosis and therapy

2018

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In recent years, circular RNAs (circRNAs) have attracted considerable attention because they play a significant role in many fields of cancer biology. Additionally, it has become increasingly clear that circRNAs have the potential to make contributions to the successful application of individualized cancer medicine. This brief review introduces circRNAs by describing their potential as a biomarker and therapeutic target and discussing the possible strategies to target them. This review also presents the challenges that are encountered by circRNAs for their definitive entry into clinical practice. Clearly, our understanding of circRNAs helps to add a new dimension to the molecular structure of cancer and will provide many new opportunities for cancer treatment.

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“…CircRNAs are formed by back-splicing covalently joined 3’- and 5’-ends (Zhang X. et al, 2017 ) and play a role in several cellular functions, including protein binding, RNA transport, and the regulation of translation (Zhang X. et al, 2017 ). Recently, it has been suggested that circRNAs play a critical role in late-stage gastric cancer (Fang et al, 2017 ). However, the influence of circRNAs in glioma needs further investigation.…”

Section: Introductionmentioning

confidence: 99%

NFIX Circular RNA Promotes Glioma Progression by Regulating miR-34a-5p via Notch Signaling Pathway

Zhang

Ling

et al. 2018

Front. Mol. Neurosci.

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Objective: The present study aimed to explore the association between NFIX circular RNA (circNFIX) and miR-34a-5p in glioma. Furthermore, this study investigated the influence that circNFIX has on glioma progression through the upregulation of NOTCH1 via the Notch signaling pathway by sponging miR-34a-5p.Methods: We applied five methods, CIRCexplorer2, circRNA-finder, CIRI, find-circ and MapSplice2, to screen for circRNAs with differential expression between three glioma tissue samples and three paired normal tissue samples. The GSEA software was used to confirm whether significantly different pathways were activated or inactivated in glioma tissues. The binding sites between circNFIX and miR-34a-5p were confirmed by TargetScan. QRT-PCR and western blot were used to measure the relative expression levels of circNFIX, miR-34a-5p and NOTCH and identify their correlation in glioma. RNA immunoprecipitation (RIP) validated the binding relationship between circNFIX and miR-34a-5p, while the targeted relationship between NOTCH1 and miR-34a-5p was verified by a dual luciferase reporter assay. Cell viability and mobility were examined by a CCK-8 assay and wound healing assay, and a flow cytometry assay was employed to analyze cell apoptosis. The nude mouse transplantation tumor experiment verified that si-circNFIX exerted a suppressive effect on glioma progression in vivo.Results: Twelve circRNAs were differentially expressed between the tissue types. Of those, circNFIX was the sole circRNA to be overexpressed in glioma among the five methods of finding circRNAs. In addition, the Notch signaling pathway was considerably upregulated in tumor tissues compared with the paired normal brain tissues. It was determined that circNFIX acted as a sponge of miR-34a-5p, a miRNA that targeted NOTCH1. Downregulation of circNFIX and upregulation of miR-34a-5p both inhibited cell propagation and migration. Furthermore, a miR-34a-5p inhibitor neutralized the suppressive effect of si-circNFIX on glioma cells. Si-circNFIX and miR-34a-5p mimics promoted cell apoptosis. Moreover, it was demonstrated in vivo that si-circNFIX could suppress glioma growth by regulating miR-34a-5p and NOTCH1.Conclusion: CircNFIX was markedly upregulated in glioma cells. CircNFIX could regulate NOTCH1 and the Notch signaling pathway to promote glioma progression by sponging miR-34a-5p via the Notch signaling pathway. This finding provided a deeper insight into the function of circNFIX in human glioma cancer progression.

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Circular RNAs play an important role in late-stage gastric cancer: Circular RNA expression profiles and bioinformatics analyses

Cited by 19 publications

References 47 publications

<p>Identification of circRNA-miRNA-mRNA Networks for Exploring the Fundamental Mechanism in Lung Adenocarcinoma</p>

<p>Identification of circRNA-miRNA-mRNA Networks for Exploring the Fundamental Mechanism in Lung Adenocarcinoma</p>

Circular RNAs: a new frontier for cancer diagnosis and therapy

NFIX Circular RNA Promotes Glioma Progression by Regulating miR-34a-5p via Notch Signaling Pathway

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