Circular RNAs in physiology and non-immunological diseases

Chen, Liang; Huang, Chuan; Ge, Shengfang

doi:10.1016/j.tibs.2021.11.004

Cited by 41 publications

(64 citation statements)

References 97 publications

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Section: Circrna-modulated Alternative Splicing In Cancermentioning

confidence: 99%

“…Circular RNAs (circRNAs) are naturally endogenous covalently closed RNA molecules back-spliced from pre-mRNA or other RNA circularization mechanisms [ 66 , 67 ]. In contrast to linear RNAs, circRNAs are resistant to RNA exonuclease, due to their loop structures, which provide them with promising features to act as potential biomarkers or therapeutic targets [ 66 , 67 ]. In general, reverse complementary sequences such as Alu elements in the flanking region of circularized exons and various RBPs such as hnRNP L, QKI and hnRNPM are responsible for circRNA biogenesis [ 68–72 ].…”

Section: Circrna-modulated Alternative Splicing In Cancermentioning

confidence: 99%

“…In general, reverse complementary sequences such as Alu elements in the flanking region of circularized exons and various RBPs such as hnRNP L, QKI and hnRNPM are responsible for circRNA biogenesis [ 68–72 ]. Accumulating evidence has demonstrated that circRNAs play essential and pivotal roles in tumorigenesis through distinct mechanisms including serving as miRNA sponges, interacting with RBPs, transcriptional regulation and acting as templates for translation [ 66 , 67 , 73 ]. For instance, we have found that circRNA ( circURI1 ) could modulate AS to engage in cancer progression and metastasis [ 24 ] ( Fig.…”

Section: Circrna-modulated Alternative Splicing In Cancermentioning

confidence: 99%

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Mechanisms of non-coding RNA-modulated alternative splicing in cancer

Wang

Jia

et al. 2022

RNA Biology

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Alternative splicing (AS) is a common and pivotal process for eukaryotic gene expression regulation, which enables a precursor RNA to produce multiple transcript variants with diverse cellular functions. Aberrant AS represents a hallmark of cancer, engaged in all stages of tumorigenesis from initiation to metastasis. Accumulating pieces of evidence have revealed the involvement of non-coding RNAs (ncRNAs) in regulating AS in human cancers. In this review, we overview the underlying mechanisms of non-coding RNAs, including microRNAs (miRNAs), long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) modulated AS at diverse levels in human cancers, and summarize their regulatory functions in tumorigenesis.

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Section: Circrna-modulated Alternative Splicing In Cancermentioning

confidence: 99%

Section: Circrna-modulated Alternative Splicing In Cancermentioning

confidence: 99%

Section: Circrna-modulated Alternative Splicing In Cancermentioning

confidence: 99%

See 1 more Smart Citation

Mechanisms of non-coding RNA-modulated alternative splicing in cancer

Wang

Jia

et al. 2022

RNA Biology

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“…Circular RNAs (circRNAs) are back-spliced from precursor mRNA (pre-mRNA) formed with exons or introns, in which a downstream splice site is joined with an upstream splice site [ 8 – 12 ]. Reverse complementary sequences such as Alu elements in the flanking region of circularized exons and RNA binding proteins (RBPs) are the major factors contributing to circRNA biogenesis [ 13 – 17 ]. Due to its covalent closed loop structure and lack of exposed terminal ends, circRNAs are resistant to the degradation by exonucleases and highly stable in blood and other body fluids [ 10 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

CircVAPA promotes small cell lung cancer progression by modulating the miR-377-3p and miR-494-3p/IGF1R/AKT axis

Jia

Wang

et al. 2022

Mol Cancer

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Background Multiple lines of evidence have demonstrated that circular RNAs (circRNAs) play oncogenic or tumor-suppressive roles in various human cancers. Nevertheless, the biological functions of circRNAs in small cell lung cancer (SCLC) are still elusive. Methods CircVAPA (annotated as hsa_circ_0006990) was identified by mining the circRNA profiling dataset of six paired SCLC tissues and the RNA-seq data of serum samples from 36 SCLC patients and 118 healthy controls. The circVAPA expression level was evaluated using quantitative real-time PCR in SCLC cells and tissues. Cell viability, colony formation, cell cycle and apoptosis analysis assays and in vivo tumorigenesis were used to reveal the biological roles of circVAPA. The underlying mechanism of circVAPA was investigated by Western blot, RNA pulldown, RNA immunoprecipitation, dual-luciferase reporter assay and rescue experiments. Results We revealed that circVAPA, derived from exons 2-4 of the vesicle-associated membrane protein-associated protein A (VAPA) gene, exhibited higher expression levels in SCLC cell lines, clinical tissues, and serum from SCLC patients than the controls, and facilitated SCLC progression in vitro and in vivo. Mechanistically, circVAPA activated the phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT) signaling pathway by modulating the miR-377-3p and miR-494-3p/insulin-like growth factor 1 receptor (IGF1R) axis to accelerate SCLC progression. Furthermore, circVAPA depletion markedly enhanced the inhibitory effects of BMS-536924, an IGF1R kinase inhibitor in cellular and xenograft mouse models. Conclusions CircVAPA promotes SCLC progression via the miR-377-3p and miR-494-3p/IGF1R/AKT axis. We hope to develop clinical protocols of combinations of circVAPA inhibition and BMS-536924 addition for treating SCLC with circVAPA upregulation.

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“…Different from other non-coding RNAs, circRNAs are not vulnerable to the degradation of RNA enzymes and are more stable than linear RNAs ( Jeck et al, 2013 ). CircRNA has a variety of molecular functions in sponge miRNA, regulatory transcription, regulatory RNA binding proteins, and even coding proteins ( Chen et al, 2021 ). What gets the most attention is that some circRNAs contain miRNA response elements (MREs), making them miRNA sponges that reduce miRNA binding to mRNA ( Hansen et al, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Integrated Analysis of circRNA-miRNA-mRNA ceRNA Network in Cardiac Hypertrophy

et al. 2022

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Cardiac hypertrophy is an adaptive cardiac response that accommodates the variable hemodynamic demands of the human body during extended periods of preload or afterload increase. In recent years, an increasing number of studies have pointed to a potential connection between myocardial hypertrophy and abnormal expression of non-coding RNAs. Circular RNA (circRNA), as one of the non-coding RNAs, plays an essential role in cardiac hypertrophy. However, few studies have systematically analyzed circRNA-related competing endogenous RNA (ceRNA) regulatory networks associated with cardiac hypertrophy. Therefore, we used public databases from online prediction websites to predict and screen differentially expressed mRNAs and miRNAs and ultimately obtained circRNAs related to cardiac hypertrophy. Based on this result, we went on to establish a circRNAs-related ceRNA regulatory network. This study is the first to establish a circRNA-mediated ceRNA regulatory network associated with myocardial hypertrophy. To verify the results of our analysis, we used PCR to verify the differentially expressed mRNAs and miRNAs in animal myocardial hypertrophy model samples. Our findings suggest that three mRNAs (Col12a1, Thbs1, and Tgfbr3), four miRNAs (miR-20a-5p, miR-27b-3p, miR-342-3p, and miR-378a-3p), and four related circRNAs (circ_0002702, circ_0110609, circ_0013751, and circ_0047959) may play a key role in cardiac hypertrophy.

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Circular RNAs in physiology and non-immunological diseases

Cited by 41 publications

References 97 publications

Mechanisms of non-coding RNA-modulated alternative splicing in cancer

Mechanisms of non-coding RNA-modulated alternative splicing in cancer

CircVAPA promotes small cell lung cancer progression by modulating the miR-377-3p and miR-494-3p/IGF1R/AKT axis

Integrated Analysis of circRNA-miRNA-mRNA ceRNA Network in Cardiac Hypertrophy

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