Circular RNAs expression profiles in human gastric cancer

Dang, Yuan; Ouyang, Xiaojuan; Zhang, Fan; Wang, Kai; Lin, Youdong; Sun, Baochang; Wang, Yu; Wang, Lie; Huang, Qiuyuan

doi:10.1038/s41598-017-09076-6

Cited by 76 publications

(61 citation statements)

References 29 publications

(27 reference statements)

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“…Results of the circRNA microarray in the present study revealed a new circRNA expression pro le in human GC, and the differentially expressed circRNA detected in this study showed a signi cant difference compared to those reported in other studies [24,32]. This study showed that 445 circRNAs were signi cantly dysregulated in GC.…”

Section: Discussionsupporting

confidence: 57%

“…Of these, only hsa_circ_0000026 expression was downregulated by a fold change of 2.8 in GC as detected by qRT-PCR, and this difference was signi cant. Dang et al [32] screened the expression pro les of ve pairs of GC and matched non-GC tissues and found that 713 circRNAs were differentially expressed in GC, of which 191 and 522 were upregulated and downregulated, respectively. Shen et al [33] performed circRNA microarray analysis and stated that 347 upregulated and 603 downregulated circRNAs were observed in GC compared with normal gastric tissue.…”

Section: Discussionmentioning

confidence: 99%

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Circular RNA hsa_circ_000780 has been identified by genomic expression profile analysis as a potential diagnostic marker for gastric cancer

Song

Zhong

et al. 2020

Preprint

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Background : The present study attempted to detect a specific circular RNA (circRNA) for the early diagnosis of gastric cancer (GC). Methods: We selected four patients with GC for this study. The total RNA of the malignant and adjacent tissues was extracted, and the circRNA was screened. The eight most upregulated and downregulated circRNAs with a statistically significant relation to GC and paired adjacent nontumorous tissues were identified using real-time fluorescent quantitative polymerase chain reaction (PCR). CircRNA expression was identified by quantitative reverse transcriptase PCR in 78 cases of GC and adjacent tissues, and in the gastric fluids of 30 patients with chronic nonatrophic gastritis, 30 patients with chronic atrophic gastritis, 21 with early GC, and 57 with advanced GC. Results: A total of 445 circRNAs, including 69 upregulated and 376 downregulated circRNAs, showed significant aberrant expression in GC tissues. A majority of the differentially expressed circRNAs originated from chr1, chr3, chr4, chr6, and chr11. Hsa_circ_000780 was significantly downregulated in 80.77% of GC tissues, and its level in GC tissues correlated with the tumor size, tumor stage, T stage, venous invasion, carcinoembryonic antigen, and carbohydrate antigen 19-9 expression. A crucial observation was the presence of this circRNA in the gastric fluid of patients with early and advanced GC. Conclusions: The present study uncovered a new hsa_circRNA expression profile in human GC, of which hsa_circ_000780 was significantly downregulated in GC tissues and in gastric fluid. It can be potentially used as a novel biomarker for early GC screening.

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Section: Discussionsupporting

confidence: 57%

Section: Discussionmentioning

confidence: 99%

Circular RNA hsa_circ_000780 has been identified by genomic expression profile analysis as a potential diagnostic marker for gastric cancer

Song

Zhong

et al. 2020

Preprint

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“…A recent study underscored the important roles of circRNAs in human GC by their ability to modulate cancer‐related pathways, linear RNA transcription and, protein expression 16 . The strategic role of hsa‐circ‐000684 in particular has not been well‐defined in previous reports.…”

Section: Discussionmentioning

confidence: 99%

Oncogenic circular RNA Hsa‐circ‐000684 interacts with microRNA‐186 to upregulate ZEB1 in gastric cancer

et al. 2020

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Circular RNAs (circRNAs) function as modulators of microRNAs (miRNAs) and are often involved in cancer progression. This study aims to investigate the underlying mechanism by which circRNA hsa‐circ‐000684 promotes the progression of gastric cancer (GC). Expression of hsa‐circ‐000684 and that of ZEB1 mRNA were elevated while microRNA‐186 (miR‐186) expression was downregulated in GC cell lines and clinical tissues. In addition, the effects of hsa‐circ‐000684 on the proliferation, migration, invasion, and tube formation of GC cells were examined through gain‐and loss‐of‐function experiments. Furthermore, we introduced tumor xenografts into nude mice to better understand these effects in vivo. Either knockdown of hsa‐circ‐000684 or upregulation of miR‐186 inhibited GC cell proliferation, migration, invasion, and tube formation in vitro, and reduced the xenograft tumor growth in nude mice. Moreover, we found that hsa‐circ‐000684 and ZEB1 directly bound to miR‐186. Hsa‐circ‐000684 increased the expression of ZEB1 by binding to miR‐186. The tumor suppressive effects of hsa‐circ‐000684 knockdown were reversed by inhibition of miR‐186. Collectively, our data show that hsa‐circ‐000684 reduces the miR‐186 expression which leads to an increase in the ZEB1 expression and, consequently, promotion of GC progression.

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“…Similarly, compared with the normal tissues, 522 downregulated and 191upregulated circRNAs were identified in gastric cancer. Further online functional analysis showed that these dysregulated circRNAs were involved in carcinogenesis [45]. Radiotherapy is a primary method of oncotherapy.…”

Section: Dysregulated Circrnas In Digestive Cancersmentioning

confidence: 99%

Regulatory mechanisms and clinical perspectives of circRNA in digestive system neoplasms

Sun¹,

Chen²,

Su³

et al. 2019

J. Cancer

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A new star, circular RNA (circRNA), is a class of noncoding RNA with a stable cyclic structure. Exonic circRNA mainly exists in the eukaryotic cytoplasm. Intronic circRNAs (ciRNA) and exonic circRNAs with introns (EIciRNA) are found in the nucleus. Recent evidences showed the functional diversity of circRNAs, which could be microRNA (miRNA) sponges, interact with protein or translate into small peptide. Due to the change of human eating habits, digestive cancer remains one of the most common cancers worldwide and it is prone to metastasis. Increasing studies have found a number of circRNAs using RNA sequencing technology and displayed double roles of circRNA in digestive cancer. In this review, we surveyed the biogenesis and regulation of circRNAs, discussed circRNA functions and clinical applications (especially circRNAs in exosome) in digestive cancers, which implied that circRNAs could be as potential biomarkers in diagnosis and treatment of digestive cancers in the future.

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Circular RNAs expression profiles in human gastric cancer

Cited by 76 publications

References 29 publications

Circular RNA hsa_circ_000780 has been identified by genomic expression profile analysis as a potential diagnostic marker for gastric cancer

Circular RNA hsa_circ_000780 has been identified by genomic expression profile analysis as a potential diagnostic marker for gastric cancer

Oncogenic circular RNA Hsa‐circ‐000684 interacts with microRNA‐186 to upregulate ZEB1 in gastric cancer

Regulatory mechanisms and clinical perspectives of circRNA in digestive system neoplasms

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