Circular RNA_0000629 Suppresses Bladder Cancer Progression Mediating MicroRNA-1290/CDC73

Abstract

Background Recent studies showed circular RNAs (circRNAs) played regulatory roles in bladder cancer (BC). However, the relevance of circ_0000629, a newly identified circRNA, has not been determined yet. We aimed to characterize the function of circ_0000629 in BC and the relevant mechanism. Methods First, we downloaded circRNA-related microarrays GSE147985 and GSE92675 from the GEO database, followed by a validation in our clinically obtained samples. We then overexpressed circ_0000629 in T24 and SW780 cells and evaluated the effects of circ_0000629 on BC cell proliferatory, apoptotic, and metastatic abilities. We further detected the subcellular localization of circ_0000629 in T24 and SW780 cells by the fractionation and export assay and FISH experiments. Integrated microarray analyses and bioinformatics website prediction were utilized to screen out the downstream microRNA (miRNA)/mRNA. The effects of miR-1290 and CDC73 on BC cell growth and metastasis was verified by functional rescue experiments. In addition, mice xenografts were built to measure the effect of circ_0000629 on tumor growth in vivo. Results Circ_0000629 and CDC73 were reduced, and miR-1290 was significantly overexpressed in BC tissues and cells. Moreover, circ_0000629 significantly inhibited the development and metastasis of BC cells, but further overexpression of miR-1290 or knockdown of CDC73 attenuated the inhibitory effect of circ_0000629 on BC cells. Circ_0000629 localized in the cytoplasm and regulated CDC73 expression by sponging miR-1290. Further, overexpressed circ_0000629 reduced the BC tumor growth in vivo. Conclusion Circ_0000629 promotes the expression of CDC73 by competitively binding to miR-1290, thereby inhibiting the growth and metastasis of BC cells.

Circular RNA circβ‐catenin aggravates the malignant phenotype of non‐small‐cell lung cancer via encoding a peptide

“…Upregulation of circRNAs can change the characteristics of tumor 31‐34 . The characteristics of tumor include many aspects, such as proliferation, migration, invasion, and drug resistance 28,29,35,36 . Therefore, we aimed to explore whether circβ‐catenin upregulation could change the characteristics of NSCLC cells.…”

“…CircRNAs have received widespread attention in recent years 46,47 . The extensive expression patterns of circRNAs strongly illuminate their role in oncogenic regulation 33,35,46 . CircRNAs are ubiquitous in the occurrence and development of NSCLC.…”

“…46,47 The extensive expression patterns of cir-cRNAs strongly illuminate their role in oncogenic regulation. 33,35,46 CircRNAs are ubiquitous in the occurrence and development of NSCLC. For example, the expression of circSMARCA5 is negatively correlated with the tumor size of NSCLC, lymph node metastasis and TNM staging.…”

“…[31][32][33][34] The characteristics of tumor include many aspects, such as proliferation, migration, invasion, and drug resistance. 28,29,35,36 Therefore, we aimed to explore whether circβ-catenin upregulation could change the characteristics of NSCLC cells. Circβ-catenin expression was upregulated in A549 and H1299 cells transfected with Lv-circβ-catenin (Figure 2A).…”

The Emerging Functions of Circular RNAs in Bladder Cancer

“…Tan et al proved that circST6GALNAC6 inhibited EMT and BC metastasis partly via the miR-200a-3p/STMN1axis [ 142 ]. Overexpression of circ_0000629 resulted in a significant increase in E-cadherin expression and a remarkable decrease in Vimentin, Snail, and N-cadherin expression, which inhibited the aggressiveness of BC [ 143 ]. CircPICALM served as a sponge for miR-1265 to modulate STEAP4 and further affected the condition of pFAK-Y397 and EMT, thus inhibiting BC progression [ 144 ].…”