Circular RNA-ZNF609 regulates retinal neurodegeneration by acting as miR-615 sponge

Wang, Jia Jian; Liu, Chang; Shan, K. Y.; Liu, Bai Hui; Li, Xiu Miao; Zhang, Shu Jie; Zhou, Rong; Dong, Rui; Yan, Biao; Sun, Xing

doi:10.7150/thno.25156

Cited by 70 publications

(54 citation statements)

References 38 publications

(53 reference statements)

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“…This hypothesis was confirmed in the following, as the cytoprotective impacts of cZNF609 silence towards HaCaT cells were alleviated when miR-145 level was suppressed by the specific inhibitor. Previously, cZNF609 has been found to be the molecular sponges of a small fraction of miRNAs, such as miR-615 [33], miR-138-5p [17], miR-150-5p [34] and miR-145 [35]. Our findings agree with the previous observations.…”

Section: Discussionsupporting

confidence: 93%

Anti-antioxidant impacts of circZNF609 silence in HaCaT cells through regulating miR-145

Gao

2020

Artificial Cells, Nanomedicine, and Biotechnology

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Background: CircZNF609 (cZNF609) is previously revealed as an essential mediator in oxidative stress. This paper determined the role of cZNF609 in skin oxidative damage to evaluate its importance in pressure ulcer. Methods: HaCaT cells treated by H 2 O 2 were considered as a cell model of pressure ulcer. The role of cZNF609 in the model was checked by conducting CCK-8 assay, FITC-PI double-staining, ROS detection and Western blot. The downstream gene and signalling of cZNF609 were studied by utilizing qRT-PCR and Western blot. Results: HaCaT cells were remarkably damaged by H 2 O 2 , as evidenced by the viability loss, apoptosis and ROS generation. It was coupled with the elevated expression of p53, p16, Bax and the activated forms of caspase-3 and PARP. Meanwhile, cZNF609 was high-expressed in response to H 2 O 2. The oxidative stress driven by H 2 O 2 was alleviated by transfection with cZNF609 specific siRNA. Further, the antiantioxidant impacts of cZNF609 silence were impeded by miR-145 silence. The inhibition of JNK and p38MAPK pathways induced by cZNF609 silence was impeded by miR-145 silence. Conclusion: The protective function of cZNF609 silence in H 2 O 2-injured HaCaT cells was revealed in vitro. Silence of cZNF609 exhibited its impact possibly through regulating miR-145, and JNK and p38MAPK pathways.

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Section: Discussionsupporting

confidence: 93%

Anti-antioxidant impacts of circZNF609 silence in HaCaT cells through regulating miR-145

Gao

2020

Artificial Cells, Nanomedicine, and Biotechnology

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“…In other words, ecircRNA formation was associated with alternative splicing in the linear mRNAs [51,55,114]. In addition, the expression of host genes and other genes could be regulated by circRNAs through sponging various miRNAs targeting different genes [115][116][117]. Furthermore, cir-cRNAs could bind to proteins to regulate the transcription of their locus genes.…”

Section: Regulation Of Transcription and Splicingmentioning

confidence: 99%

Circular RNAs and their roles in head and neck cancers

et al. 2019

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Circular RNAs are abundant endogenous non-coding RNA with no 5′ cap and 3′ polyadenylation tail that modify liner mRNAs and have no terminal structures. Our knowledge of the biogenesis of circular RNAs has been expanded, and circular RNAs were shown to be key regulators of various diseases, especially cancers. Head and neck cancers are the sixth most popular cancers worldwide, and the overall survival rates remain unsatisfactory. Recent studies have indicated that circular RNAs are involved in the tumorigenesis, progression, invasion and chemosensitivity of head and neck cancers and that some circular RNAs could serve as diagnostic and prognostic biomarkers. In this study, we summarize research advances in the regulation of circular RNA biogenesis, their characteristics and functions, the involvement of circular RNAs in the pathophysiology of head and neck cancers and their potential clinical utilization, as well as the likely directions of future studies.

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“…Wang et al revealed that cZNF609 is significantly upregulated in glaucoma-related retinal neurodegeneration. Retinal reactive gliosis and glial cell activation are inhibited by cZNF609 silencing ( Wang et al, 2018a ). They also found that cZRANB1 is potentially evolved in retinal degeneration induced by COH.…”

Section: Introductionmentioning

confidence: 99%

Circular RNA Expression Profiling Identifies Glaucoma-Related Circular RNAs in Various Chronic Ocular Hypertension Rat Models

et al. 2020

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Circular RNAs are characterized as a class of covalently closed circular RNA transcripts and are associated with a variety of cellular processes and neurological diseases by sponging microRNAs. Expression profiling of circular RNAs in glaucoma, which is a form of optic neuropathy, has not been performed to date. The most common characteristic of all forms of glaucoma is the loss of retinal ganglion cells. While the pathogenesis of glaucoma is not fully understood, intraocular pressure is unquestionably the only proven modifiable factor which makes chronic ocular hypertension (COH) animals the classical glaucoma models. Based on these findings, we completed the first in-depth study of rat retinal circular RNA expression profiling to identify probable biomarkers for the diagnosis of glaucoma. Two ocular hypertension models were induced by episcleral vein ligation (EVL) and microbead injection in rats. Overall, 15,819 circular RNA were detected. Furthermore, 3,502 differentially expressed circular RNAs verified in both COH rats were identified, of which 691 were upregulated and 2,811 were downregulated. Seven significantly downregulated (both log 2 FoldChange < −2.5 and adjusted P < 0.001) and seven significantly upregulated (both log 2 FoldChange > 2.5 and adjusted P < 0.001) circular RNAs were shown. Six target microRNAs aligned with the top 14 circular RNAs were identified. According to the construction of the circular RNA-microRNA network and circBase information, only RNO_CIRCpedia_1775 had the homologous hsa_circ_0023826 in the human genome. The hsa_circ_0023826 and mRNA of the host gene TENM4 (teneurin transmembrane protein 4) were validated in aqueous humor samples of five glaucoma patients and five cataract control patients. The expression of hsa_circ_0023826 showed a significant decrease in glaucoma patients, while TENM4 mRNA showed no significant difference compared to cataract patients ( P = 0.024 and P = 0.294, respectively). The results of this study comprehensively characterized the expression profiles of circular RNA in glaucoma-affected eyes, as verified by two different ocular hypertension rat models. Together with the target microRNAs underlying the top differentially expressed circular RNAs, a new target of hsa_circ_0023826 and its host gene TENM4 were identified and further verified in the aqueous humor of glaucoma patients, indicating a promising biomarker for the disease.

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Circular RNA-ZNF609 regulates retinal neurodegeneration by acting as miR-615 sponge

Cited by 70 publications

References 38 publications

Anti-antioxidant impacts of circZNF609 silence in HaCaT cells through regulating miR-145

Anti-antioxidant impacts of circZNF609 silence in HaCaT cells through regulating miR-145

Circular RNAs and their roles in head and neck cancers

Circular RNA Expression Profiling Identifies Glaucoma-Related Circular RNAs in Various Chronic Ocular Hypertension Rat Models

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