Circular RNA VMA21 ameliorates sepsis‐associated acute kidney injury by regulating miR‐9‐3p/SMG1/inflammation axis and oxidative stress

Shi, Yan; Sun, Chuan-Fu; Ge, Wenhan; Du, Yeping; Hu, Nan-Bin

doi:10.1111/jcmm.15741

Cited by 60 publications

(44 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Accumulating evidence stated that numerous circRNAs are closely associated with multiple human diseases [ 15 ], including sepsis [ 16 ]. For example, circVMA21 played a crucial part in sepsis-associated AKI via microRNA-9-39 (miR-9-39) [ 17 ]. Circ-FANCA (hsa_circ_0040994) is produced from precursor mRNA FANCA, and it is identified to be highly expressed in sepsis-stimulated AKI [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Knockdown of circ-FANCA alleviates LPS-induced HK2 cell injury via targeting miR-93-5p/OXSR1 axis in septic acute kidney injury

Li¹,

Zhang

Wang

et al. 2021

Diabetol Metab Syndr

View full text Add to dashboard Cite

Background Sepsis is life-threatening disease with systemic inflammation and can lead to various diseases, including septic acute kidney injury (AKI). Recently, diverse circular RNAs (circRNAs) are considered to be involved in the development of this disease. In this study, we aimed to elucidate the role of circ-FANCA and the potential action mechanism in sepsis-induced AKI. Methods HK2 cells were treated with lipopolysaccharide (LPS) to establish septic AKI cell model. The expression of circ-FANCA, microRNA-93-5p (miR-93-5p) and oxidative stress responsive 1 (OXSR1) mRNA was determined by quantitative real-time polymerase chain reaction (qRT-PCR). Cell viability was assessed using cell counting kit-8 (CCK-8) assay. Cell apoptosis and cell cycle distribution were measured by flow cytometry. The inflammatory response was monitored according to the release of pro-inflammatory cytokines via enzyme-linked immunosorbent assay (ELISA). The activities of oxidative indicators were examined using the corresponding kits. Dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay were applied to validate the interaction between miR-93-5p and circ-FANCA or OXSR1. Protein analysis was conducted through western blot. Results Circ-FANCA was upregulated in septic AKI serum specimens and LPS-treated HK2 cells. Functionally, circ-FANCA knockdown facilitated cell proliferation and restrained apoptosis, inflammation and oxidative stress in LPS-triggered HK2 cells. Further mechanism analysis revealed that miR-93-5p was a target of circ-FANCA and circ-FANCA modulated LPS-induced cell damage by targeting miR-93-5p. Meanwhile, miR-93-5p overexpression repressed LPS-treated HK2 cell injury by sponging OXSR1. Furthermore, circ-FANCA regulated OXSR1 expression by sponging miR-93-5p. Besides, exosome-derived circ-FANCA was upregulated in LPS-induced HK2 cells, which was downregulated by GW4869. Conclusion Circ-FANCA knockdown attenuated LPS-induced HK2 cell injury by regulating OXSR1 expression via targeting miR-93-5p.

show abstract

Section: Introductionmentioning

confidence: 99%

Knockdown of circ-FANCA alleviates LPS-induced HK2 cell injury via targeting miR-93-5p/OXSR1 axis in septic acute kidney injury

Li¹,

Zhang

Wang

et al. 2021

Diabetol Metab Syndr

View full text Add to dashboard Cite

show abstract

“…Fang et al proved that lncRNA-H19 increase the expression of AQP1 through competitive binding miRNA-874 in sepsis, restoring dysregulated in ammatory responses [31]. Circular RNA VMA21 ameliorated sepsis-associated acute kidney injury via regulating miR-9-39/SMG1/in ammation axis and oxidative stress [32]. In this study, lncRNA/circRNA-miRNA-mRNA network was also constructed to discovery the regulatory relations of these ncRNAs and mRNAs.…”

Section: Discussionmentioning

confidence: 90%

Whole Transcriptome Analysis on Sepsis Caused by Pyogenic Liver Abscess

Zhao¹,

Liu²,

Han³

et al. 2021

Preprint

View full text Add to dashboard Cite

Background Pyogenic liver abscess (PLA) is a common clinical disease throughout the world with remaining high mortality and morbidity. This study sought to investigate key genes and non-coding RNAs (ncRNAs) associated with the pathogenesis and treatment of sepsis caused by PLA (SLA). Methods We constructed the expression profiles of RNAs by RNA sequencing in the peripheral blood of healthy subjects, SLA and seven day therapy of SLA patients. Then the sequencing data was analysed through integrated bioinformatics strategy. Results 4549 mRNAs, 9808 lncRNAs, 467 circRNAs and 292 miRNAs were differentially expressed (DE) between SLA and control samples. 3199 mRNAs, 6018 lncRNAs, 161 circRNAs and 132 miRNAs were differentially expressed between SLA after 7 days therapy and SLA control subjects. Moreover, DE mRNAs of the former two comparisons were both related to immunity and T cell-mediated immune response; DE lncRNAs were both associated with B cell receptor signaling pathway and Osteoclast differentiation; DE circRNAs were both related to Chagas disease and B cell receptor signaling pathway; DE miRNAs exerts its specific role in the process of SLA by MAPK signaling pathway and Estrogen signaling pathway compared to DE lncRNAs, circRNAs and mRNAs. Finally, lncRNA/circRNA–miRNA–mRNA network was constructed to explore the internal regulating relationships of these ncRNAs and mRNAs. Conclusions Our results may provide a new perspective on differential RNA expression involved in the pathogenesis and treatment of SLA and accelerate the development of novel treatments for this disease.

show abstract

“…The expression of circ-0114427 is upregulated and can resist the early inflammatory state, by binding to miR-494 as an miRNA sponge to decrease ATF3 expression and further increase IL-6 expression ( Cao et al, 2020 ). Similar, circVMA21 alleviated sepsis-associated AKI via regulating miR-9-3p/SMG1/inflammation and oxidative stress ( Shi et al, 2020 ).…”

Section: Circular Rnas In Acute Kidney Injurymentioning

confidence: 97%

“…Gpnmb Naglu NF-κB activation Inflammation circ_0114427 (Cao et al, 2020) miR-494 AFT3 NF-κB activation circ-AKT3 (Xu et al, 2020) miR-144-5p Wnt/β-catenin pathway Wnt/β-catenin activation circ-YAP1 (Huang et al, 2020) miR-21-5p PI3K/AKT/mTOR pathway PI3K/AKT/mTOR activation circ-VMA21 (Shi et al, 2020) miR-9-3p SMG1 Oxidative stress Chronic kidney disease circHLA-C (Luan et al, 2018) miR-150 Undefined Fibrosis-associated genes express circRNA_15698 (Hu et al, 2019) miR-185 TGF-β1 Extracellular matrix-related protein synthesis circ_WBSCR17 (Li et al, 2020a) miR-185-5p SOX6 ECM accumulation circRNA_002453 (Ouyang et al, 2018) Undefined Undefined Biomarker in blood circ_DLGAP4 (Bai et al, 2020) miR-145 ERBB3/NF-κB/MMP-2 pathway Inflammation circLRP6 miR-205 HMGB 1/TLR4/NF-κB pathway Inflammation circRNA_010383 (Peng et al, 2020) miR-135a TRPC1 ECM accumulation circ-AKT3 (Tang et al, 2020) miR-296-3p E-cadherin ECM accumulation circNr1h4 (Lu et al, 2020) miR-155-5p Far1 Reactive oxygen cANRIL (Deng et al, 2019) miR-9 NF-κB and JNK/p38 pathways Inflammation Renal cell carcinom has_circ_001895 miR-296-5p SOX12 Promoting proliferation, migration, invasion, inhibiting apotosis circEGLN3 (Lin and Cai, 2020) miR-1299 IRF7 Promoting proliferation, migration, invasion, inhibiting apotosis circNRIP1 (Dong et al, 2020) miR-505 AMPK and PI3KL/AKT/mTOR pathways Promoting proliferation, migration, invasion, inhibiting apotosis circNUP98 (Yu et al, 2020) miR-567 PRDX3 Promoting proliferation, migration, invasion, inhibiting apotosis has_circ_0072309 miR-100 PI3KL/AKT/mTOR Inhibiting proliferation, migration, invasion, promoting apotosis circC3P1 miR-21/PTEN PI3K/AKT and NF-κB Inhibiting proliferation, migration, invasion, promoting apotosis circFNDC3B miR-99a JAK1/STAT3 and MEK/ERK pathways Promoting proliferation, migration circ-ZNF652 (Zhang and Guo, 2020) miR-205 JAK1/STAT3 and MEK/ERK pathways Promoting proliferation, migration circ-ZNF609 (Xiong et al, 2019) miR-138-5p FOXP4 Promoting proliferation, invasion circDHX33 (Wang et al, 2020) miR-498-3p MERK Promoting proliferation, invasion circHIPK3 miR-508-3p CXCL13 Promoting proliferation, migration, invasion circUBAP2 (Sun et al, 2020) miR-148a-3p FOXK2 Promoting proliferation, migration, invasion circ_001842 (Zeng et al, 2020) miR-502-5p SLC39A14 Promoting proliferation, migration, invasion cRAPGEF5 miR-27a-3p TXNIP Inhibiting proliferation, migration has_circ_0001451 associated genes (Zhou et al, 2013;…”

Section: Circular Rnas In Chronic Kidney Diseasesmentioning

confidence: 99%

Role of Circular RNA in Kidney-Related Diseases

Chen

Zhao

et al. 2021

Front. Pharmacol.

View full text Add to dashboard Cite

The kidney is vital in maintaining fluid, electrolyte, and acid–base balance. Kidney-related diseases, which are an increasing public health issue, can happen to people of any age and at any time. Circular RNAs (circRNAs) are endogenous RNA that are produced by selective RNA splicing and are involved in progression of various diseases. Studies have shown that various kidney diseases, including renal cell carcinoma, acute kidney injury, and chronic kidney disease, are linked to circRNAs. This review outlines the characteristics and biological functions of circRNAs and discusses specific studies that provide insights into the function and potential of circRNAs for application in the diagnosis and treatment of kidney-related diseases.

show abstract

Circular RNA VMA21 ameliorates sepsis‐associated acute kidney injury by regulating miR‐9‐3p/SMG1/inflammation axis and oxidative stress

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 60 publications

References 36 publications

Knockdown of circ-FANCA alleviates LPS-induced HK2 cell injury via targeting miR-93-5p/OXSR1 axis in septic acute kidney injury

Knockdown of circ-FANCA alleviates LPS-induced HK2 cell injury via targeting miR-93-5p/OXSR1 axis in septic acute kidney injury

Whole Transcriptome Analysis on Sepsis Caused by Pyogenic Liver Abscess

Role of Circular RNA in Kidney-Related Diseases

Contact Info

Product

Resources

About