Circular RNA Ttc3 regulates cardiac function after myocardial infarction by sponging miR-15b

Cai, Lingling; Qi, Baozhen; Wu, Xiaoyu; Peng, Shi; Zhou, Genqing; Wei, Yong; Xu, Juan; Chen, Songwen; Liu, Shaowen

doi:10.1016/j.yjmcc.2019.03.007

Cited by 115 publications

(90 citation statements)

References 33 publications

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“…Among them, circ-Ttc3 resulted in one of the top highest expressed circRNAs in mouse heart (Tan et al, 2017). Cai L. et al (2019) found that circTtc3 was markedly upregulated in the ischemic myocardium, whereas there was no significant change in linear Ttc3 RNA. Overexpression of circ-Ttc3 in cardiomyocytes counteracted hypoxia-induced ATP depletion and apoptotic death.…”

Section: Circrnas In Skeletal and Cardiac Myogenesismentioning

confidence: 99%

Non-coding RNAs Shaping Muscle

Martone

Mariani

Desideri

et al. 2020

Front. Cell Dev. Biol.

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In 1957, Francis Crick speculated that RNA, beyond its protein-coding capacity, could have its own function. Decade after decade, this theory was dramatically boosted by the discovery of new classes of non-coding RNAs (ncRNAs), including long ncRNAs (lncRNAs) and circular RNAs (circRNAs), which play a fundamental role in the fine spatio-temporal control of multiple layers of gene expression. Recently, many of these molecules have been identified in a plethora of different tissues, and they have emerged to be more cell-type specific than protein-coding genes. These findings shed light on how ncRNAs are involved in the precise tuning of gene regulatory mechanisms governing tissues homeostasis. In this review, we discuss the recent findings on the mechanisms used by lncRNAs and circRNAs to sustain skeletal and cardiac muscle formation, paying particular attention to the technological developments that, over the last few years, have aided their genome-wide identification and study. Together with lncRNAs and circRNAs, the emerging contribution of Piwi-interacting RNAs and transfer RNA-derived fragments to myogenesis will be also discussed, with a glimpse on the impact of their dysregulation in muscle disorders, such as myopathies, muscle atrophy, and rhabdomyosarcoma degeneration.

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Section: Circrnas In Skeletal and Cardiac Myogenesismentioning

confidence: 99%

Non-coding RNAs Shaping Muscle

Martone

Mariani

Desideri

et al. 2020

Front. Cell Dev. Biol.

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“…Oppositely, downregulating circ‐Ttc3 deteriorated myocardial dysfunction after myocardial infarction. Overall, circ‐Ttc3 performed its function via sponging miR‐15b . Circ‐Ttc3 expression was raised by hypoxia treatment.…”

Section: Discussionmentioning

confidence: 97%

“…Besides, a previous finding explored that circRNA Tetratricopeptide repeat domain 3 (circ‐Ttc3) was observed to be upregulated in hypoxic cardiomyocytes. Furthermore, circ‐Ttc3 showed protected effect in H9c2 cells . Therefore, we hypothesized that circ‐Ttc3 performed the similar function on PU.…”

Section: Introductionmentioning

confidence: 94%

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Circular RNA circ‐Ttc3 protects HaCaT cells from hypoxic injury by downregulation of miR‐449a

Wang

Liu

et al. 2020

IUBMB Life

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Background Pressure ulcer (PU) is a common complication of acute and long‐time hospitalization, especially in elderly patients. The incidence and prevalence of PU are swiftly fortifying. Currently, our purpose was to investigate the functional impacts of circ‐Ttc3 on PU. Methods HaCaT cells were pretreated under hypoxia condition. Cell counting kit 8 assay and flow cytometry were utilized to test HaCaT cell viability and apoptosis. Quantitative reverse transcription polymerase chain reaction was utilized to determine circ‐Ttc3 and miR‐449a expression. Western blot was performed to examine apoptosis‐associated proteins expression. Subsequently, the above‐described investigations were reperformed after miR‐449a upregulation. Results Hypoxia induced apoptosis and declined viability in HaCaT cells. Circ‐Ttc3 expression was enhanced after transfection with circ‐Ttc3 expressing vector. Overexpressing circ‐Ttc3 raised viability and reduced apoptosis in HaCaT cells. Moreover, miR‐449a expression was elevated by hypoxia and reversed by overexpressing circ‐Ttc3. And circ‐Ttc3 exerted its effect via downregulating miR‐449a. Finally, overexpressing circ‐Ttc3 activated nuclear factor kappa‐B (NF‐κB) and phosphatidylinositol 3 kinase (PI3K)/protein kinase B (AKT) pathways via downregulating miR‐449a. Conclusion Our data suggested that circ‐Ttc3 alleviated hypoxic injury and activated NF‐κB and PI3K/AKT pathways by downregulating miR‐449a in HaCaT cells.

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“…Most researchers reported that circRNAs are in charge of regulating gene expression principally by binding with and then resulting in miRNAs dysfunction [10]. A mount of reports veri ed the crucial functions of circRNAs in CVDs [16]. Abnormally expressed circRNAs were strongly linked to the occurrence and progress of CVDs, for instance, hypertrophy, heart failure and atherosclerosis [17,18], etc.…”

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confidence: 99%

The Expression Profiles and Role of Circular RNA in Peripheral Blood of Myocardial Infarction Patients

Li¹,

Li³

et al. 2020

Preprint

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Background: Myocardial infarction (MI) is one of the most widespread illnesses seriously harmful to human health. However, the mechanisms of its pathogenesis have not yet been completely proved. circRNA is one kind of non-coding RNA, recently published data suggests that circRNAs play a crucial role in heart diseases, but their expression profiles and role in MI patients are not yet completely defined. Methods: RNAs from peripheral blood samples were recruited for high-throughput RNA-seq analysis; The junction site part of circRNAs was confirmed by PCR with divergent primers; ROC curves were created to estimate the diagnostic value of circRNAs for MI; The expression level of circRNAs in clinical and cell samples were detected by RT-qPCR; MiRNA target site in exons of circRNA loci was verified by using miRanda, The binding sites of miRNA and mRNA were predicted by RNAhybrid; The regulatory roles of circSLC8A1 in cardiomyocytes were detected by TUNEL and Immunoblotting.Results: A total of 3862 circRNAs were detected to be remarkably changed, including 2738 circRNAs being upregulated, 1124 circRNAs being downregulated. circTMEM165, circUBAC2, circZNF609, circANKRD12, and circSLC8A1 were upregulated in MI and had closed loop structure. The results of ROC curves revealed that these five circRNAs had good sensitivity and specificity for MI diagnosis. Moreover, we created networks among circRNAs, miRNAs and mRNAs to predict the regulatory roles of circRNAs in MI. Besides that, we also demonstrated that circSLC8A1 contributed to cardiomyocytes apoptosis. Conclusions: In this study, we depicted the expression profiles of circular RNA in peripheral blood of myocardial infarction patients, revealed the five circRNAs may be very beneficial for MI diagnosis and regarded as possible biomarkers. Moreover, we also revealed that circSLC8A1 played a critical role in cardiomyocytes apoptosis.

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Circular RNA Ttc3 regulates cardiac function after myocardial infarction by sponging miR-15b

Cited by 115 publications

References 33 publications

Non-coding RNAs Shaping Muscle

Non-coding RNAs Shaping Muscle

Circular RNA circ‐Ttc3 protects HaCaT cells from hypoxic injury by downregulation of miR‐449a

The Expression Profiles and Role of Circular RNA in Peripheral Blood of Myocardial Infarction Patients

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