Circular RNA SMARCA5 suppressed non-small cell lung cancer progression by regulating miR-670-5p/RBM24 axis

Abstract

Circular RNAs (circRNAs) have good stability and long half-life in blood and other body fluid, and possess regulatory effects on various biological processes as miRNA/RNA-binding protein sponges, or by competing endogenous RNA, indicating their great potential as biomarkers or targets of cancer therapy. In this study, we mainly explored the role and mechanism of circular RNA SMARCA5 (circsSMARCA5) in non-small cell lung cancer (NSCLC). Quantitative RT-PCR was applied to measure the expression levels of genes, and then, the relationships among circsSMARCA5, microRNA-670-5p (miR-670-5p), and RBM24 were further analyzed. Animal and cell experiments were performed to explore the functions of circsSMARCA5 in NSCLC cells. The results showed that circsSMARCA5 was expressed at low level in NSCLC tissues and cells, while miR-670-5p had high level in NSCLC tissues. Dual luciferase reporter assay verified that miR-670-5p was the target of circsSMARCA5, and RBM24 has the binding site of miR-670-5p. Further analysis showed that circsSMARCA5 could negatively regulate miR-670-5p and had positive relationship with RBM24. Moreover, circsSMARCA5 obviously inhibited tumor growth in vivo, reduced cell proliferation and increased cell apoptosis in vitro, while miR-670-5p mimic or RBM24 knockdown could reverse these effects. Thus, circsSMARCA5 may serve as an NSCLC suppressor by regulating the miR-670-5p/RBM24 axis, and it may have the potential to be a biomarker or therapeutic target for NSCLC.

Tumor suppressor RBM24 inhibits nuclear translocation of CTNNB1 and TP63 expression in liver cancer cells

“…In the present study, we first validated that RBM24 exhibited strong tumor-suppressive potential in liver cancer. These results consistent with the tumor-suppressive role of RBM24 in other cancer types, including lung, prostate, and nasopharyngeal carcinomas (4,5,10). In fact, we observed that HepG2 cells did not show the suppression of sphere formation by RBM24 expression, which may indicate the cell type-specific regulation of RBM24.…”

“…Moreover, several oncogenes and tumor suppressor genes control RBPs in various cancer cell lines ( 9 ). RBM24, an RBP with a single conserved RNA recognition motif domain, is involved in post-transcriptional regulation and has been shown to influence the proliferation and motility of various cancer cells ( 4 , 5 , 10 ). Previous studies have demonstrated that RBM24 may regulate the stability of p21 and p63 mRNA transcripts in different human cancer cell lines ( 11 , 12 ).…”

Role and Mechanism of circ_0058063/miR-635 Axis in the Malignant Phenotype of Multiple Myeloma RPMI8226 Cells

“…Circular RNA (circRNA) and microRNA (miRNA) are two types of noncoding RNAs. As competitive endogenous RNA, circRNA together with miRNA regulate miRNA target genes expression and then affect cell proliferation, apoptosis, and migration, which has close relationship with the occurrence and development of tumor [ 2 , 3 ]. Research has shown that circ_0058063 expression is upregulated in esophageal squamous cell carcinoma, knocking down its expression can reduce the esophageal squamous cell carcinoma proliferation rate through inhibiting GLUT1 expression [ 4 ]; circ_0058063 is overexpressed in bladder cancer tissues.…”

miR-20a-5p/RBM24 Axis Alleviated Hypertensive Intracerebral Hemorrhage via Regulating HIF1α/VEGFA Signaling Pathway

“…In the present study, we found that RBM24 is a direct target of miR-20a-5p. It has been demonstrated that RBM24 played important roles in many progresses of various diseases, such as cell alternative splicing, lung cancer progression and skeletal muscle regeneration [27,28]. In addition, it also proved that RBM24 could regulated by miRNAs.…”