Circular RNA expression profiles in umbilical cord blood exosomes from normal and gestational diabetes mellitus patients

Cao, Mingtao; Zhang, Le; Lin, Yongcheng; Li, Zhengying; Xu, Jianjuan; Shi, Zhonghua; Chen, Zhong; Ma, Jinqi; Wen, Juan

doi:10.1042/bsr20201946

Cited by 28 publications

(25 citation statements)

References 32 publications

(35 reference statements)

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“…Except for lncRNAs and miRNAs, circRNA was also found in EVs; these were discovered to carry signaling molecules for cellular communication and even organ crosstalk. A recent study has shown different expression patterns of exosomal circRNAs between GDM patients and healthy pregnant women ( 111 ). They identified 507 differentially expressed circRNAs in GDM patients compared with controls, and reported that several pathways were significantly enriched based on GO and KEGG analyses, including the pentose phosphate pathway, cholesterol metabolism, galactose metabolism, as well as other pathways ( 111 ).…”

Section: Role Of Non-coding Rnas and Evs In Gdmmentioning

confidence: 99%

“…A recent study has shown different expression patterns of exosomal circRNAs between GDM patients and healthy pregnant women ( 111 ). They identified 507 differentially expressed circRNAs in GDM patients compared with controls, and reported that several pathways were significantly enriched based on GO and KEGG analyses, including the pentose phosphate pathway, cholesterol metabolism, galactose metabolism, as well as other pathways ( 111 ). Therefore, the roles of circRNAs in EVs are still limited and needs to be further verified by animal and clinical studies.…”

Section: Role Of Non-coding Rnas and Evs In Gdmmentioning

confidence: 99%

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Non-Coding RNAs and Extracellular Vehicles: Their Role in the Pathogenesis of Gestational Diabetes Mellitus

et al. 2021

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Gestational diabetes mellitus (GDM) is defined as glucose intolerance with onset or first recognition in the second or third trimester of pregnancy. GDM has a considerable impact on health outcomes of the mother and offspring during pregnancy, delivery, and beyond. Although the exact mechanism regarding GDM remains unclear, numerous studies have suggested that non-coding RNAs, including long non-coding (lnc)RNAs, microRNAs, and circular RNAs, were involved in the pathogenesis of GDM in which they played vital regulatory roles. Additionally, several studies have revealed that extracellular vehicles also participated in the pathogenesis of GDM, highlighting their important role in this disease. Considering the lack of effective biomarkers for the early identification of and specific treatment for GDM, non-coding RNAs and extracellular vehicles may be promising biomarkers and even targets for GDM therapies. This review provides an update on our understanding of the role of non-coding RNAs and extracellular vehicles in GDM. As our understanding of the function of lncRNAs and extracellular vehicles improves, the future appears promising for their use as potential biomarkers and treatment targets for GDM in clinical practice.

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Section: Role Of Non-coding Rnas and Evs In Gdmmentioning

confidence: 99%

Section: Role Of Non-coding Rnas and Evs In Gdmmentioning

confidence: 99%

Non-Coding RNAs and Extracellular Vehicles: Their Role in the Pathogenesis of Gestational Diabetes Mellitus

et al. 2021

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“…Although the molecular mechanisms involved in its pathophysiology are not fully elucidated, ncRNAs appear to have an important role in the pathogenic mechanisms of GDM (reviewed by [ 31 ]). Indeed, in the last few months, the impressive number of studies reporting an association between GDM and different classes of ncRNAs, including miRNAs [ 85 , 86 , 87 ]; circRNAs [ 52 , 88 , 89 ]; and long ncRNAs [ 90 ], support the possible role of ncRNAs as an early predictor of the disease. Nevertheless, a few studies have investigated the ncRNA pattern according to the fetal sex ( Table 3 ).…”

Section: Ncrnas In Pregnancy Complications: Role Of Fetal Sexmentioning

confidence: 99%

Significance of Sex Differences in ncRNAs Expression and Function in Pregnancy and Related Complications

et al. 2021

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In the era of personalized medicine, fetal sex-specific research is of utmost importance for comprehending the mechanisms governing pregnancy and pregnancy-related complications. In recent times, noncoding RNAs (ncRNAs) have gained increasing attention as critical players in gene regulation and disease pathogenesis, and as candidate biomarkers in human diseases as well. Different types of ncRNAs, including microRNAs (miRNAs), piwi-interacting RNAs (piRNAs), long noncoding RNAs (lncRNAs), and circular RNAs (circRNAs), participate in every step of pregnancy progression, although studies taking into consideration fetal sex as a central variable are still limited. To date, most of the available data have been obtained investigating sex-specific placental miRNA expression. Several studies revealed that miRNAs regulate the (patho)-physiological processes in a sexually dimorphic manner, ensuring normal fetal development, successful pregnancy, and susceptibility to diseases. Moreover, the observation that ncRNA profiles differ according to cells, tissues, and developmental stages of pregnancy, along with the complex interactions among different types of ncRNAs in regulating gene expression, strongly indicates that more studies are needed to understand the role of sex-specific ncRNA in pregnancy and associated disorders.

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“…Exosomal circRNAs have been supported to be the promising non-invasive diagnostic biomarkers for various diseases, such as cancers and metabolic diseases [9]. A recent microarray study has revealed that there are differential exosomal circRNA expression pro les in unbilical cord blood between GDM patients and controls, implying the signi cant value of circRNAs in GDM development and fetal growth [10]. Hsa_circRNA_0054633 has been found to be highly expressed in the blood samples of GDM patients and exhibited a potential diagnostic value in the middle and advanced stage of pregnancy [11].…”

Section: Introductionmentioning

confidence: 99%

Circulating Exosomal hsa_circRNA_0039480 is Highly Expressed in Gestational Diabetes Mellitus and May Be Served As A Biomarker for Early Diagnosis of GDM

Jiang

Zhang

Sun

et al. 2021

Preprint

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Background: Gestational diabetes mellitus (GDM) seriously affects the health of mothers and babies, and there are still no effective early diagnostic markers. Therefore, it is necessary to find diagnostic biomarkers for screening GDM in early pregnancy. Circular RNA (circRNA) is more stable than linear RNA, and can be encapsulated in exosomes and participate in the pathological process of various diseases, which makes it a better candidate biomarker for various diseases. In this study, we attempted to identify the exosomal circRNA biomarkers for detecting early GDM.Methods: We performed microarray analysis to compare the plasma exosomal circRNA expression profiles of three GDM patients 48 hours before and 48 hours after delivery. The repeatability of the expression of circRNAs were randomly validated by RT-PCR analysis. Pearson correlation analysis was applied to evaluate the correlation between circRNAs and circRNA-OGTT level. ROC curve was established to assess the diagnostic value of circRNAs for GDM at different stages.Results: Plasma exosomal hsa_circRNA_0039480 and hsa_circRNA_0026497 were highly expressed in GDM patients before delivery(P<0.05). The hsa_circRNA_0039480 expression was higher for GDM group than NGT group at different stages, and was also positively correlated with OGTT during the second trimester (P<0.05). The expression of hsa_circRNA_0026497 was higher for GDM group during the third, and second trimesters. And there was a strong correlation between two circRNAs in GDM patients during the first-trimester (r =0.496, P=0.014). Hsa_circRNA_0039480 showed significant diagnostic value in the first, second, and third trimesters of pregnancy (AUC = 0.704, P=0.005; AUC = 0.898, P < 0.001 and AUC = 0.698, P= 0.001, respectively). Notably, the combination of hsa_circRNA_0039480 and hsa_circRNA_0026497 exhibited promising discriminative effect on GDM in the first trimesters (AUC=0.754, P < 0.001).Conclusion: Plasma exosomal hsa_cirRNA_0039480 is highly expressed in GDM patients and may be served as a candidate biomarker for early detection of GDM.

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Circular RNA expression profiles in umbilical cord blood exosomes from normal and gestational diabetes mellitus patients

Cited by 28 publications

References 32 publications

Non-Coding RNAs and Extracellular Vehicles: Their Role in the Pathogenesis of Gestational Diabetes Mellitus

Non-Coding RNAs and Extracellular Vehicles: Their Role in the Pathogenesis of Gestational Diabetes Mellitus

Significance of Sex Differences in ncRNAs Expression and Function in Pregnancy and Related Complications

Circulating Exosomal hsa_circRNA_0039480 is Highly Expressed in Gestational Diabetes Mellitus and May Be Served As A Biomarker for Early Diagnosis of GDM

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