Circular RNA expression profile in human fibroblast premature senescence after repeated ultraviolet B irradiations revealed by microarray

Abstract

Circular RNA (circRNA) is a class of noncoding RNA that regulates the activity of microRNAs and gene expression. Altered circRNA expression is associated with human diseases. The present study profiled differentially expressed circRNAs in the ultraviolet B stress‐induced human fibroblast premature senescence (UVB‐SIPS) model, and assessed the role of circRNA_100797 in UVB‐SIPS. The UVB‐SIPS model was confirmed by ß‐galactosidase staining, cell viability CCK‐8 assay, and flow cytometric cell cycle distribution assay, and subjected to circRNA gene chip profiling. These differentially expressed circRNAs were analyzed using the clusterProfiler R package for Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) database pathways. The selected circRNAs were confirmed using quantitative reverse transcription polymerase chain reaction (qRT‐PCR), the relationship of circRNA_100797 with miR‐23a‐5p was assessed using luciferase reporter assay, and their functions were determined by qRT‐PCR and western blot analysis. A total of 472 differentially expressed circRNAs occurred in the UVB‐SIPS. qRT‐PCR confirmed five of eight differentially expressed circRNAs. The GO and KEGG analyses revealed that these differently expressed circRNAs function in biology process, cell component, and molecular function. Furthermore, it was found that circRNA_100797 had a low expression in UVB‐SIPS. However, when circRNA_100797 was overexpressed, the acceleration of cell proliferation and alleviation of cell cycle arrest were observed. Moreover, circRNA_100797 could target miR‐23a‐5p, their expression levels were inversely associated in fibroblasts, and the miR‐23a‐5p overexpression blocked the effect of the overexpression of circRNA_100797 in UVB‐SIPS. The present study demonstrated that circRNA_100797 acts as a sponge of miR‐23a‐5p, and has a photoprotection role in UVB‐irradiated fibroblasts.

The Role of microRNAs in Organismal and Skin Aging

“…Besides, in the model of the UVB stress-induced premature senescence (UVB-SIPS) of human fibroblasts, it was found that the overexpression of miR-23a-5p reduced cellular proliferation and accelerated a cell cycle arrest. At the same time, in UVB-SIPS fibroblasts it had an opposite effect on the photoprotective circulatory RNA_100797, which acted as a sponge of miR-23a-5p [ 207 ].…”

CircASH2L Promotes Ovarian Cancer Tumorigenesis, Angiogenesis, and Lymphangiogenesis by Regulating the miR-665/VEGFA Axis as a Competing Endogenous RNA

“…Circular RNA (circRNA) is a type of endogenous RNA that forms a covalently closed continuous loop structure without a 5 -cap or a 3 -poly A tail (Wang et al, 2019c;Wei et al, 2020). Owing to its structure, circRNA is more stable than linear RNA and can be resistant to exonucleolytic RNA decay (Si et al, 2019;Liu T. et al, 2020). CircRNA is involved in the regulation of many diseases by interacting with disease-associated microRNA (miRNA) as a competing endogenous RNA (ceRNA) (Wang et al, 2018;Zhang Y. et al, 2019;Li H. et al, 2020).…”

Dental Pulp Stem Cell-Derived Extracellular Vesicles Mitigate Haematopoietic Damage after Radiation

“…Some roles of these microRNA have been revealed, such as the miR-125a-5p can affect cell migration and modulate sensitivity to ionizing radiation [32], the miR-143-3p increases in exosome-like EVs from irradiated whole blood samples and contributes to early stages of radiation-induced genomic instability [33,34], the miR-199a-5p works as a novel and unique regulator of radiation induced-autophagy [35], and the miR-455-3p regulates cell growth, metastasis, and glycolysis in hepatocellular carcinoma [36]. The miR-23a-5p blocks the effect of the acceleration of cell proliferation and alleviation of cell cycle arrest [37], and the miR-1894-3p works as an important antimetastatic miRNA in lung metastasis [38].…”