Circular RNA ciRS-7 promotes the proliferation and metastasis of pancreatic cancer by regulating miR-7-mediated EGFR/STAT3 signaling pathway

Liu, Lei; Liu, Fubao; Huang, Mei; Xie, Kun; Xie, Qing; Liu, Chenhai; Shen, Minjing; Huang, Qiang

doi:10.1016/j.hbpd.2019.03.003

Cited by 110 publications

(86 citation statements)

References 29 publications

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“…Emerging evidence suggests that dysregulation of circRNAs plays a pivotal role in the development of malignancies . Our results showed that up‐regulation circPRMT5 was a commonly oncogenic event in CRC, contributing to the cell proliferation by regulating cell cycle‐associated proteins cyclin D1 and CDK2.…”

Section: Discussionmentioning

confidence: 58%

“…This study demonstrated that circPRMT5 was an important up-regulation circRNA in CRC, and higher expression of circPRMT5 showed poorer survival of CRC patients. CircPRTM5 exerts critical oncogenic role in CRC by sponging miR-377 to induce E2F3 expression and promoting cell cycle-associated proteins cyclinD1 and CDK2 expression.Emerging evidence suggests that dysregulation of circRNAs plays a pivotal role in the development of malignancies [17][18][19][20]. Our results showed that up-regulation circPRMT5 was a commonly oncogenic event in CRC, contributing to the cell proliferation by regulating cell cycle-associated proteins cyclinD1 and CDK2.…”

mentioning

confidence: 52%

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CircPRMT5 circular RNA promotes proliferation of colorectal cancer through sponging miR‐377 to induce E2F3 expression

Yang

et al. 2020

J Cellular Molecular Medi

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CircPRTM5 is associated with cell proliferation and migration in many kinds of malignancies. However, the functions and mechanisms of CircPRTM5 in CRC progression remain unclear. We explored the role and the mechanisms of CircPRTM5 in the development of CRC. Tissues of CRC patients and matched adjacent non‐tumour tissues were collected to evaluate the expression of CircPRTM5. The expression of CircPRTM5 in CRC tissues was significantly higher than that in adjacent tissues. The biological functions of CircPRTM5 in CRC were determined by overexpression and down‐regulation of CircPRTM5 in CRC cells in vitro and in vivo. The results indicate that knockdown of CircPRTM5 can significantly inhibit the proliferation of CRC cells. The potential mechanisms of CircPRTM5 in CRC development were identified by RT‐qPCR, Western blotting analysis and luciferase reporter assay. CircPRTM5 competitively regulates the expression of E2F3 by capillary adsorption of miR‐377. CircPRMT5 regulates CRC proliferation by regulating the expression of E2F3, which affects the expression of the cell cycle‐associated proteins cyclinD1 and CDK2. CircPRTM5 exerts critical regulatory role in CRC progression by sponging miR‐377 to induce E2F3 expression.

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Section: Discussionmentioning

confidence: 58%

mentioning

confidence: 52%

CircPRMT5 circular RNA promotes proliferation of colorectal cancer through sponging miR‐377 to induce E2F3 expression

Yang

et al. 2020

J Cellular Molecular Medi

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“…The circRNA ciRS7 is expressed in PDAC tissues compared to para-cancerous tissues and can negatively regulate miR-7, a cancer suppressor. It can also affect the proliferation and invasion of PDAC cells through epidermal growth factor receptor (EGFR), as well as signal transducer and activator of transcription 3 (STAT3) signaling pathways (77). In addition, overexpression of ciRS7 promotes lymph node metastasis and venous invasion in PDAC cases.…”

Section: Circrnas Regulate Pdac Progressionmentioning

confidence: 99%

Non-coding RNAs in Pancreatic Ductal Adenocarcinoma

Gong

Jiang

2020

Front. Oncol.

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“…circITCH absorbed miR-17 and miR-224 to promote p21 and PTEN expression in bladder cancer 26 . ciRS-7 targeted miR-7 to promote EGFR/STAT3 signaling, while circZMYM2 sponged miR335-5p and in turn favored the expression of JMJD2C in PDAC 27,28 . Here, we performed bioinformatics analysis, luciferase assay and miRNA pull down assay to identify circFOXK2-interacting miRNAs in PDAC cells.…”

Section: Discussionmentioning

confidence: 99%

CircFOXK2 Promotes Tumor Growth and Metastasis of Pancreatic Ductal Adenocarcinoma via Complexing with RNA Binding Proteins and Sponging MiR-942

Wong

Lou

et al. 2019

Preprint

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Objective: Circular RNA (circRNA) is a novel class of non-coding RNAs that regulate gene expression. However, the role of circRNAs in pancreatic ductal adenocarcinoma (PDAC) is largely unknown. Design:We performed circRNA sequencing of non-tumor HPDE and PDAC cells. We investigated the functions of circFOXK2 in PDAC by gain-of-function and loss-of-function assays. Bioinformatics analysis, luciferase assay and microRNA pulldown assays were performed to identify circFOXK2 interacting-miRNAs. To further investigate the mechanism, we performed circRNA-pulldown and mass spectrometry to identify circFOXK2-interacting proteins in PDAC. Results:We identified 169 differentially expressed circRNAs in PDAC cells. We validated that one of the circRNAs circFOXK2 was significantly up-regulated in PDAC cells and in 63 % of primary tumor (53 out of 84). Gain-of-function and loss-of-function assays demonstrated that circFOXK2 promoted PDAC cell growth, migration and invasion. CircFOXK2 was also involved in cell cycle progression and apoptosis. circFOXK2 functioned as sponge for miR-942, and in turn promoted the expression of miR-942 targets ANK1, GDNF and PAX6. Furthermore, circFOXK2 interacted with 94 proteins, which were involved in cell adhesion and mRNA splicing. Among these circFOXK2-interacting proteins, YBX1 and hnRNPK were validated by RNA immunoprecipitation. Importantly, circFOKX2 interacted with YBX1 and hnRNPK targets NUF2 and PDXK in PDAC cells. Knockdown of circFOXK2 reduced the binding of YBX1 and hnRNPK to NUF2 and PDXK, and in turn decreased their expressions in PDAC cells. Conclusion:We identified that circFOXK2 promoted PDAC cells growth and metastasis. Also, circFOXK2 complexed with YBX1 and hnRNPK to promote the expressions of oncogenic proteins.• circFOXK2 upregulation in PDAC may function as a novel biomarker for diagnosis.• circFOXK2 may be a novel therapeutic target in treating PDAC.

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Circular RNA ciRS-7 promotes the proliferation and metastasis of pancreatic cancer by regulating miR-7-mediated EGFR/STAT3 signaling pathway

Cited by 110 publications

References 29 publications

CircPRMT5 circular RNA promotes proliferation of colorectal cancer through sponging miR‐377 to induce E2F3 expression

CircPRMT5 circular RNA promotes proliferation of colorectal cancer through sponging miR‐377 to induce E2F3 expression

Non-coding RNAs in Pancreatic Ductal Adenocarcinoma

CircFOXK2 Promotes Tumor Growth and Metastasis of Pancreatic Ductal Adenocarcinoma via Complexing with RNA Binding Proteins and Sponging MiR-942

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