Circular RNA circZNF652 is overexpressed in osteoarthritis and positively regulates LPS-induced apoptosis of chondrocytes by upregulating PTEN

Abstract

BackgroundCircular RNA circZNF652 promotes LPS-induced in ammation, which contributes to the development of osteoarthritis (OA), indicating the potential involvement of CRNDE in OA. This study was carried to explore the involvement of circZNF652 in OA. Methods RT-qPCR was performed to analyze the expression of circZNF652 and PTEN mRNA in synovial uid samples from 60 OA patients and 60 healthy controls. Correlations between circZNF652 and PTEN mRNA were analyzed by Pearson's correlation coe cient. Overexpression and siRNA silencing of circZNF652 were achieved in chondrocytes, followed by performing RT-qPCR and Western blot to analyze the expression of PTEN. The role of circZNF652 and PTEN in regulating the apoptosis of chondrocytes induced by LPS was analyzed by cell apoptosis assay. Results We found that circZNF652 was overexpressed in OA and positively correlated with PTEN mRNA. In chondrocytes, circZNF652 overexpression increased the expression of PTEN, and circZNF652 siRNA silencing decreased the expression of PTEN. Moreover, circZNF652 and PTEN positively regulated the apoptosis of chondrocytes induced by LPS. PTEN overexpression reversed the inhibitory effects of circZNF652 siRNA silencing on cell apoptosis. ConclusionCircZNF652E is overexpressed in OA and positively regulates LPS-induced apoptosis of chondrocytes by upregulating PTEN.

Extracellular Vesicles from Hypoxic Pretreated Urine-Derived Stem Cells Enhance the Proliferation and Migration of Chondrocytes by Delivering miR-26a-5p

“… 66 , 67 However, little is reported about its role in OA. Yuan et al 35 found that PTEN is overexpressed in OA and positively regulates the apoptosis of chondrocytes induced by lipopolysaccharide (LPS). Another study reported that PTEN negatively regulated Col2a1 and Aggrecan in chondrocytes.…”

“… 33 , 34 Recent studies showed that PTEN overexpression induced the apoptosis of chondrocytes and inhibited chondrocytes growth. 35 , 36 This study attempted to investigate the mechanism of USC-EVs in OA. We also explored whether hypoxic preconditioning could enhance the beneficial effects of USC-EVs on chondrocytes.…”

The Role of Regulated Programmed Cell Death in Osteoarthritis: From Pathogenesis to Therapy

“…Many non-coding RNAs, including long-noncoding RNAs and circular RNAs, can regulate chondrocyte apoptosis through different mechanisms. Some of the mechanisms that can promote chondrocyte apoptosis involve Circ_0136474 [ 60 , 61 ], long non-coding RNA plasmacytoma variant translocation 1 [ 62 ], LINC00707 [ 63 ], and CircZNF652E [ 64 ]; the mechanisms that can prevent chondrocyte apoptosis include circANKRD36 [ 65 ], long noncoding RNA NAV2-AS5 [ 66 ], and circ_0020014 [ 67 ]. Circular RNAs act as sponges for miRNAs to suppress their activity and, thereby, regulate the expression of their downstream target genes [ 68 ].…”

The Role of Non-Coding RNAs in the Pathogenesis of Parkinson’s Disease: Recent Advancement

“…The relationship between circRNAs and inflammation is now gaining recognition, and some studies have revealed that circRNAs participate in diabetes mellitus [ 241 ], atherosclerosis [ 242 ], osteoarthritis [ 243 ], and spinal cord injury [ 244 ], all of which are associated with immunoinflammatory effects. However, circRNA profiling and the regulatory mechanisms of neuroinflammation in PD remain unclear.…”