Circular RNA circNASP modulates the malignant behaviors in osteosarcoma via miR-1253/FOXF1 pathway

Huang, Lipeng; Chen, Mangmang; Pan, Jun; Yu, Weiyang

doi:10.1016/j.bbrc.2018.04.131

Cited by 56 publications

(41 citation statements)

References 27 publications

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“…cyclic RNAs have been found to be involved in tumor progression in various cancer types. circular RNAs have been confirmed to exert various effects, including functioning as biomarkers to improve the diagnostic efficiency and serving as a regulatory factor in OS progression (12,(31)(32)(33). In the study by Liu et al, it was reported that circNT52 functioned as an oncogene to regulate OS cell proliferation and metastasis by sponging miR-448 (34).…”

Section: Discussionmentioning

confidence: 99%

“…Studies have also verified that circular RNAs, as competitive endogenous RNAs, bind to miRNAs and regulate the expression of downstream target genes (9)(10)(11). For example, circular RNA circNASP has been shown to regulate OS malignant behavior via the miR-1253/FOXF1 axis (12). circSAMd4A, a novel circular RNA, was derived from the SAMd4A gene and is located in chr14:55168799-55169298.…”

Section: Introductionmentioning

confidence: 98%

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CircSAMD4A regulates cell progression and epithelial‑mesenchymal transition by sponging miR‑342‑3p via the regulation of FZD7 expression in osteosarcoma

Xie

Chen

et al. 2020

Int J Mol Med

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Osteosarcoma (OS) is a primary malignant tumor with a complex etiology. Therefore, research into the pathogenesis of osteosarcoma is considered a priority. circular RNAs play important roles in cell metabolism and in the immune response and are closely associated with cancer treatment. However, research into the association of circular RNAs with osteosarcoma is limited. In the present study, circSAMd4A was validated by RT-qPcR and agarose gel electrophoresis. circSAMd4A and miR-342-3p expression was detected by RT-qPcR. The relative protein expression levels were measured by western blot analysis. MTT assay and flow cytometry were used to detect cell cytotoxicity and apoptosis, respectively. Transwell assay was applied to assess cell migration and invasion. dual-luciferase reporter assay was used to determine the association among circSAMd4A, Frizzled-7 (FZd7) and miR-342-3p. In vivo, subcutaneous tumor formation assay was performed in an experiment with nude mice. The results revealed that the expression levels of circSAMd4A and FZd7 were upregulated, while those of miR-342-3p were downregulated in OS tissues and cells. The inhibition of circSAMd4A suppressed cell progression and epithelial-mesenchymal transition (EMT), and promoted cell apoptosis in OS. The reduction of miR-342-3p reversed the effects of circSAMd4A downregulation on cell cytotoxicity, migration, invasion, apoptosis and EMT in OS, while FZd7 overexpression blocked the effect of miR-342-3p upregulation on OS progression. The suppressive effect of sh-circSAMd4A on tumor growth was thus verified in OS. Overall, the present study demonstrated that circSAMd4A affected cell cytotox-icity, invasion, apoptosis, migration and EMT by regulating the miR-342-3p/FdZ7 axis in OS, thereby providing a novel regulatory mechanism and a potential therapeutic target for OS.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

CircSAMD4A regulates cell progression and epithelial‑mesenchymal transition by sponging miR‑342‑3p via the regulation of FZD7 expression in osteosarcoma

Xie

Chen

et al. 2020

Int J Mol Med

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“…OS observed in patients who usually develop secondary to Paget's disease, radiation, or dedifferentiated chondrosarcomas. 2 Etiology of OS is unknown. The molecular level study on OS is still in its infancy, and the concrete mechanism remains to be further studied.…”

Section: Introductionmentioning

confidence: 99%

Transcription factor CEBPB inhibits the proliferation of osteosarcoma by regulating downstream target gene CLEC5A

Jin

Chen

Líu

et al. 2019

Clinical Laboratory Analysis

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ObjectiveTo screen the gene related to osteosarcoma (OS) metastasis and the molecular mechanism.MethodsGEO database and R2 chip analysis platform were used to screen genes related to OS metastasis. UCSC gene browser was used to find the transcription factor (TF) of CLEC5A. The mRNA level and protein expression of CLEC5A in OS tissues and normal tissues were determined by RT‐PCR and Western blotting, respectively. OS cell lines MG‐63 were transfected with CEBPB recombinant plasmid. After transfection, the expression of CLEC5A in MG‐63 cells was determined and the cell proliferation situation was determined by clone formation assay.ResultsThree genes CLEC5A, ALOX5AP, and RNASE3 were obtained, and CLEC5A has the highest correlation with OS. CLEC5A has screened the gene related to OS metastasis, and CEBPB can be taken as TF regulating downstream gene CLEC5A. CEBPB can regulate the downstream CLEC5A as transcription factor. The relative mRNA level and protein expression of CLEC5A in OS tissues were significantly higher than those in normal tissues. CLEC5A can prevent OS metastasis. Transfection of CEBPB increased the expression of CLEC5A in MG‐63 cells and also inhibited the proliferation of OS.ConclusionCEBPB can inhibit the proliferation of OS cells via regulating the expression of CLEC5A.

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“…Circular RNAs (circRNAs), a category of endogenous RNAs united by a structure of closed‐loop without a 5ʹ‐3ʹ polarity, are a promising class of non‐coding RNAs capable of regulating transcriptional or posttranscriptional gene expression via functioning as microRNAs (miRNAs) sponges . Several individual circRNAs have been revealed to play essential roles in osteosarcoma pathogenesis, such as regulating cancer cell cycle, cell proliferation, and chemoresistance, and serving as prognostic biomarker in osteosarcoma patients . CircRNA La‐related RNA‐binding protein 4 (circ‐LARP4) is a novel circRNA derived from the LARP4 gene, a gene encoding La‐related RNA‐binding protein that is capable of regulating cancer cell migration and invasion .…”

Section: Introductionmentioning

confidence: 99%

“…[4][5][6][7] Several individual circRNAs have been revealed to play essential roles in osteosarcoma pathogenesis, such as regulating cancer cell cycle, cell proliferation, and chemoresistance, and serving as prognostic biomarker in osteosarcoma patients. [8][9][10] CircRNA La-related RNA-binding protein 4 (circ-LARP4) is a novel circRNA derived from the LARP4 gene, a gene encoding Larelated RNA-binding protein that is capable of regulating cancer cell migration and invasion. 11 Furthermore, it is reported that circ-LARP4 could inhibit cancer cell proliferation and invasion in other cancers apart from osteosarcoma.…”

Section: Introductionmentioning

confidence: 99%

Circular RNA LARP4 correlates with decreased Enneking stage, better histological response, and prolonged survival profiles, and it elevates chemosensitivity to cisplatin and doxorubicin via sponging microRNA‐424 in osteosarcoma

Shen

et al. 2019

Clinical Laboratory Analysis

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Background This study aimed to evaluate the association of circular RNA La‐related RNA‐binding protein 4 (circ‐LARP4) with clinical features and prognosis in osteosarcoma patients, and further explore its effect on chemosensitivity in osteosarcoma cells. Methods Seventy‐two osteosarcoma patients with Enneking stage IIA‐IIB who underwent resection were consecutively enrolled, and then, tumor tissues and non‐tumor tissues were obtained. Circ‐LARP4 in tumor tissue/non‐tumor tissue was detected by quantitative polymerase chain reaction. After circ‐LARP4 overexpression and negative control overexpression plasmid transfection, relative cell viability (%) was evaluated by Cell Counting Kit‐8 in MG63 cells treated by different concentrations of cisplatin, methotrexate, and doxorubicin, and IC50 was calculated. Results Circ‐LARP4 was downregulated in tumor tissue compared with non‐tumor tissue and had a good value in distinguishing tumor tissue from non‐tumor tissue with an area under curve of 0.829 (95% CI: 0.762‐0.859). Meanwhile, tumor circ‐LARP4 was negatively correlated with the Enneking stage. After resection, circ‐LARP4 high expression patients showed an increased tumor cell necrosis rate to adjuvant chemotherapy compared to circ‐LARP4 low expression patients, and circ‐LARP4 high expression correlated with prolonged disease‐free survival and overall survival. In vitro experiments revealed that circ‐LARP4 overexpression elevated the chemosensitivity of MG63 cells to cisplatin and doxorubicin but not methotrexate, with decreased cisplatin IC50 and doxorubicin IC50 concentrations than negative control. Besides, miR‐424 overexpression attenuated the chemosensitivity in circ‐LARP4 overexpression‐treated MG63 cells. Conclusion Circ‐LARP4 high expression correlates with decreased Enneking stage and prolonged survival profiles, and it elevates chemosensitivity to cisplatin and doxorubicin via sponging miR‐424 in osteosarcoma.

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Circular RNA circNASP modulates the malignant behaviors in osteosarcoma via miR-1253/FOXF1 pathway

Cited by 56 publications

References 27 publications

CircSAMD4A regulates cell progression and epithelial‑mesenchymal transition by sponging miR‑342‑3p via the regulation of FZD7 expression in osteosarcoma

CircSAMD4A regulates cell progression and epithelial‑mesenchymal transition by sponging miR‑342‑3p via the regulation of FZD7 expression in osteosarcoma

Transcription factor CEBPB inhibits the proliferation of osteosarcoma by regulating downstream target gene CLEC5A

Circular RNA LARP4 correlates with decreased Enneking stage, better histological response, and prolonged survival profiles, and it elevates chemosensitivity to cisplatin and doxorubicin via sponging microRNA‐424 in osteosarcoma

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