Circular RNA circ_0124644 exacerbates the ox-LDL-induced endothelial injury in human vascular endothelial cells through regulating PAPP-A by acting as a sponge of miR-149-5p

Wang, Gang; Yu, Li; Liu, Zhenbin; Ma, Xiuna; Li, Menghu; Qu, Lu; Li, Yanfang; Lu, Zengzhen; Niu, Li Na; Fan, Zeyu; Lei, Zhangcheng

doi:10.1007/s11010-020-03764-0

Cited by 45 publications

(35 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…disease (Zhao et al, 2017) and was confirmed to promote atherosclerotic progression (Wang G. et al, 2020). In this study, a significant high circ_0124644 expression was found in the serum of AMI patients and hypoxia-induced cardiomyocytes.…”

Section: Discussionsupporting

confidence: 58%

“…In past studies, circ_0124644 [derived from roundabout guidance receptor 2 (ROBO2) gene] had been reported to be highly expressed in the serum of coronary artery disease patients and was positively correlated with disease degree (Zhao et al, 2017). In addition, high expression of circ_0124644 had been proven to promote ox-LDL-induced human vascular endothelial cell injury (Wang G. et al, 2020). However, whether circ_0124644 participates in the development of AMI has not yet been studied.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Circ_0124644 Serves as a ceRNA for miR-590-3p to Promote Hypoxia-Induced Cardiomyocytes Injury via Regulating SOX4

et al. 2021

View full text Add to dashboard Cite

Circular RNA (circRNA) is an important factor for regulating the progression of many cardiovascular diseases, including acute myocardial infarction (AMI). However, the role of circ_0124644 in AMI progression remains unclear. Hypoxia was used to induce cardiomyocytes injury. The expression of circ_0124644, microRNA (miR)-590-3p, and SRY-box transcription factor 4 (SOX4) mRNA was measured by qRT-PCR. Cell counting kit 8 (CCK8) assay and flow cytometry were utilized to detect cell viability, cell cycle progression, and apoptosis. The protein levels of apoptosis markers and SOX4 were determined by western blot (WB) analysis, and the levels of oxidative stress markers were assessed using commercial Assay Kits. Dual-luciferase reporter assay, RIP assay, and RNA pull-down assay were employed to confirm the interaction between miR-590-3p and circ_0124644 or SOX4. Circ_0124644 was upregulated in AMI patients and hypoxia-induced cardiomyocytes. Hypoxia could inhibit cardiomyocytes viability, cell cycle process, and promote apoptosis and oxidative stress, while silencing circ_0124644 could alleviate hypoxia-induced cardiomyocytes injury. In terms of mechanism, circ_0124644 could target miR-590-3p. MiR-590-3p overexpression could relieve hypoxia-induced cardiomyocytes injury. Also, the suppressive effect of circ_0124644 knockdown on hypoxia-induced cardiomyocytes injury could be reversed by miR-590-3p inhibitor. Moreover, SOX4 was found to be a target of miR-590-3p, and its overexpression also could reverse the regulation of miR-590-3p on hypoxia-induced cardiomyocytes injury. Circ_0124644 silencing could alleviate hypoxia-induced cardiomyocytes injury by regulating the miR-590-3p/SOX4 axis, suggesting that it might be a target for alleviating AMI.

show abstract

Section: Discussionsupporting

confidence: 58%

Section: Introductionmentioning

confidence: 99%

Circ_0124644 Serves as a ceRNA for miR-590-3p to Promote Hypoxia-Induced Cardiomyocytes Injury via Regulating SOX4

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Besides, hsa_circ_0003204 deletion evidently downregulates the expression of E-cadherin and upregulates the expression of vimentin and N-cadherin in oxLDL-challenged HUVECs [ 122 ]. Wang et al have noticed that circ_0124644 alleviates the endothelial injuries in ox-LDL-challenged HUVECs through the miR-149-5p/PAPP-A axis [ 123 ]. It is also shown that circ_0003645 deletion mitigates the apoptosis and inflammation in oxLDL-challenged ECs [ 124 ].…”

Section: Potential Circrnas In Aaasmentioning

confidence: 99%

Circular RNA Expression: Its Potential Regulation and Function in Abdominal Aortic Aneurysms

Han

Zhang

Bian

et al. 2021

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

Abdominal aortic aneurysms (AAAs) have posed a great threat to human life, and the necessity of its monitoring and treatment is decided by symptomatology and/or the aneurysm size. Accumulating evidence suggests that circular RNAs (circRNAs) contribute a part to the pathogenesis of AAAs. circRNAs are novel single-stranded RNAs with a closed loop structure and high stability, having become the candidate biomarkers for numerous kinds of human disorders. Besides, circRNAs act as molecular “sponge” in organisms, capable of regulating the transcription level. Here, we characterize that the molecular mechanisms underlying the role of circRNAs in AAA development were further elucidated. In the present work, studies on the biosynthesis, bibliometrics, and mechanisms of action of circRNAs were aims comprehensively reviewed, the role of circRNAs in the AAA pathogenic mechanism was illustrated, and their potential in diagnosing AAAs was examined. Moreover, the current evidence about the effects of circRNAs on AAA development through modulating endothelial cells (ECs), macrophages, and vascular smooth muscle cells (VSMCs) was summarized. Through thorough investigation, the molecular mechanisms underlying the role of circRNAs in AAA development were further elucidated. The results demonstrated that circRNAs had the application potential in the diagnosis and prevention of AAAs in clinical practice. The study of circRNA regulatory pathways would be of great assistance to the etiologic research of AAAs.

show abstract

“…For example, Qin et al ( 7 ) demonstrated that knockdown of hsa_circ_0003645 expression alleviates inflammation and apoptosis in oxidized low-density lipoprotein (oxLDL)-induced HUVECs. Moreover, Wang et al ( 8 ) indicated that circ_0124644 exacerbates oxLDL-induced injury in HUVECs via sponging miR-149-5p in atherosclerosis. In addition, hsa_circ_0001445 expression is notably downregulated in atherosclerosis ( 9 ).…”

Section: Introductionmentioning

confidence: 99%

Overexpression of hsa_circ_0001445 reverses oxLDL‑induced inhibition of HUVEC proliferation via SRSF1

et al. 2021

View full text Add to dashboard Cite

Atherosclerosis is a primary cause of multiple types of cardiovascular disease, including myocardial infarction. In addition, injury of human umbilical vein endothelial cells (HUVECs) can lead to the development of atherosclerosis. Circular (circ)RNAs participate in atherosclerosis. It has previously been shown that circRNA cSMARCA5 (hsa_ circ_0001445) expression is downregulated in atherosclerosis. However, the effects of hsa_circ_0001445 on the proliferation of HUVECs remain unclear. In order to mimic atherosclerosis in vitro, HUVECs were treated with oxidized low-density lipoprotein (oxLDL). The expression levels of specific genes and proteins were detected in HUVECs by reverse transcription-quantitative PCR and western blot analysis, respectively. Cell proliferation was assessed by Cell Counting Kit-8 and 5-Ethynyl-2'-deoxyuridine staining. Cell apoptosis and 5,5' ,6,6'-Tetrachloro-1,1' ,3,3'-tetraethyl-imidacarbocyanine staining were examined by flow cytometry. In addition, the association between hsa_circ_0001445 and serine/arginine-rich splicing factor 1 (SRSF1) was investigated by RNA pull-down assay. hsa_circ_0001445 expression was downregulated in oxLDL-treated HUVECs. Moreover, oxLDL-induced inhibition of HUVEC proliferation was significantly reversed by overexpression of hsa_circ_0001445. oxLDL notably inhibited tube formation and mitochondrial membrane potential in HUVECs, while these effects were markedly reversed by hsa_circ_0001445 overexpression. Furthermore, overexpression of hsa_circ_0001445 reversed oxLDL-induced activation of β-catenin by binding to SRSF1. Collectively, these data demonstrated that overexpression of hsa_circ_0001445 reversed oxLDL-induced inhibition of HUVEC proliferation via activation of the SRSF1/β-catenin axis. These findings may provide novel targets for the treatment of atherosclerosis.

show abstract

Circular RNA circ_0124644 exacerbates the ox-LDL-induced endothelial injury in human vascular endothelial cells through regulating PAPP-A by acting as a sponge of miR-149-5p

Cited by 45 publications

References 29 publications

Circ_0124644 Serves as a ceRNA for miR-590-3p to Promote Hypoxia-Induced Cardiomyocytes Injury via Regulating SOX4

Circ_0124644 Serves as a ceRNA for miR-590-3p to Promote Hypoxia-Induced Cardiomyocytes Injury via Regulating SOX4

Circular RNA Expression: Its Potential Regulation and Function in Abdominal Aortic Aneurysms

Overexpression of hsa_circ_0001445 reverses oxLDL‑induced inhibition of HUVEC proliferation via SRSF1

Contact Info

Product

Resources

About